Zeiss Axioskop 2 plus Operating Manual page 94

Routine microscope
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Carl Zeiss
Fig. 3-18
Setting the height stop on the
condenser carrier
(5)
Transmitted-light brightfield configuration of the Axioskop 2 mot plus
Each Axioskop 2 mot plus configuration permits the performance of the transmitted-light brightfield
technique.
(6)
Setting the Axioskop 2 mot plus for transmitted-light brightfield according to KÖHLER
− The Axioskop 2 mot plus has been set up as described in chapter 2.
− Programming of the stage movement in Z, which covers the matching of focus positions and focus
speeds of the used objectives, has been finished.
− The instrument is switched on and is in the automatic mode.
Illumination intensity, condenser settings (position of the front lens, position of the turret disk and the
opening of the aperture diaphragm) and the status "transmitted light switched on or off" are stored by
pressing the Set key.
• Setting of KÖHLER illumination to be performed in the same way as described in section 3.3.1 (3) for
the manual Axioskop 2 plus. Unlike this procedure, however, the luminous-field diaphragm must be
fully opened after the correct setting of the condenser. Shortly press the Set key on the instrument
rear after the last setting step to store the settings. A short beep is heard when the Set key is released
to indicate that storage has been performed.
• The above diaphragm settings must be repeated for all objectives, since field size and objective
aperture have different values.
For < 10x objectives, the front lens of the universal condenser must be folded out and the
aperture diaphragm fully opened.
3-26
OPERATION
Illumination and contrasting techniques
(4)
• Loosen fixation screw of height stop (3-18/1)
• Use focusing drive to focus on the specimen.
• Close luminous-field diaphragm and image it in
• Carefully lift the condenser slightly without
• Tighten fixation screw (3-18/1) of the height
Setting
the
height
condenser carrier
using SW 3 ball-headed screwdriver.
focus via the height adjustment control (3-18/2)
of the universal condenser.
touching the specimen.
adjustment.
Axioskop 2 plus
Axioskop 2 mot plus
stop
on
the
B 40-075 e
02/01

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