Zeiss Axioskop 2 plus Operating Manual page 103

Axioskop 2 plus
Axioskop 2 mot plus
3.3.4.3 Circular polarization contrast
(1) Application
Unlike polarization contrast, circular polarization contrast does not feature any dark positions which
depend on the turning angle (azimuth) of the specimen in relation to the polarizer or analyzer. All
transparent specimens feature their specific interference colors in optical anisotropy.
(2) Axioskop 2 plus and Axioskop 2 mot plus configuration
− Objectives suitable for polarization
− Rotary mechanical stage 75x50/240° R
− Circular polarizer D (polarizers must not be integrated in the condenser), including the relevant λ/4-
plate
− Analyzer module D in reflector turret
(3) Setting of Axioskop 2 plus and
Axioskop 2 mot plus
• Set the microscope as for transmitted-light
brightfield according to KÖHLER (see section
3.3.1 (3)).
• Center the rotary stage (3-25/3) (see section
2.2.9 (2)) and then remove specimen from the
stage.
• Swing circular polarizer (3-25/2) in the beam
path.
• Swing analyzer module D on reflector turret
(3-25/4). The field of view now appears dark on
account of the crossed polarizers.
• Push the upper λ/4-plate (3-25/1) into the
compartment for compensators above the
nosepiece until stop. The previously dark field of
view becomes bright again.
• Turn the λ/4-plate of the circular polarizer
(3-25/2) until optimum darkness of the field of
view is achieved. Close the aperture diaphragm
by approx. 2/3 of its diameter to increase the
dark position.
• Position the specimen to be examined on the
mechanical stage again.
B 40-075 e 02/01
OPERATION
Illumination and contrasting techniques
2
Fig. 3-25 Components for transmitted-light
polarization on the Axioskop 2 plus
Carl Zeiss
4 0
9 0
3-35