Zeiss Axio Scope.A1 Operating Manual
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Zeiss Axio Scope.A1 Operating Manual

Microscope for routine and entry-level research
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  • Page 2 Brands and names indicated in this manual may be trademarks of other companies. These brands and names are mentioned only for information and do not express any approval or recommendation by us. Carl Zeiss MicroImaging GmbH cannot assume any liability for the performance or the use of these brands.
  • Page 3 Axio Scope.A1 Content / Figures Carl Zeiss CONTENT Page INTRODUCTION ....................Safety Guidelines ......................Warranty........................Stand Variants....................... Interface Diagram ......................Summary of the Controls and Functional Elements........... TECHNICAL MANUAL ..................Intended Use ......................... Systems Overview ......................Technical Data ....................... STARTUP ......................
  • Page 4 Carl Zeiss Content / Figures Axio Scope.A1 Switching the Microscope and its Separate Illumination Fixtures on and off ..Mounting Optional Components ................3.4.1 Changing the Upper Stand Part and Inserting a Centerpiece for Larger Specimens ... 3.4.2 Mounting the Intermediate Plate for Analyzer Slider............
  • Page 5 Axio Scope.A1 Content / Figures Carl Zeiss 4.2.9 Upper Stand Part HAL 100/HBO DIC ................1 01 4.2.10 Sideport ISCP T60N Left ....................1 02 4.2.11 Reflector Slider 2-fold ....................1 03 4.2.12 Reflector Turret 4-fold or 6-fold ..................1 03 MAINTENANCE, CHANGING OF FUSES AND SERVICE ........

  • Page 6: Table Of Contents

    Carl Zeiss Content / Figures Axio Scope.A1 FIGURES F ig. 1-1 Caution sign on lower stand part for HAL 100- and HAL 50-illumination......1 8 8 H 1 8 8 H F ig. 1-2 Caution sign on upper stand part FL-LED................

  • Page 7: F Ig

    Axio Scope.A1 Content / Figures Carl Zeiss F ig. 3-41 Mounting the magnification changer .................. 4 4 H 4 4 H 2 3 2 H 2 3 2 H F ig. 3-42 Changing the filters in the reflector module FL P&C.............
  • Page 8 Carl Zeiss Content / Figures Axio Scope.A1 F ig. 4-32 Binocular photo tube ......................1 06 9 0 H 9 0 H 2 7 8 H 2 7 8 H F ig. 4-33 Adjusting the viewing height on the binocular tube ............

  • Page 9: Introduction

    INTRODUCTION Safety Guidelines The microscopes Axio Scope.A1 were engineered, manufactured and tested according to DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101, safety regulations for metering, control and laboratory equipment. The microscopes fulfill the requirements as stated in regulation 98/79/EG (In-Vitro-Diagnostika) and carry the label The present manual gives information and safety warnings which must be followed by the operator.
  • Page 10 Carl Zeiss Safety Guidelines Axio Scope.A1 The microscope Axio Scope.A1 and its genuine accessories may only be used for microscopy methods as indicated in this manual. The following guidelines are especially important: The manufacturer admits no liability if the microscope, its components or single parts, are used in any different manner.
  • Page 11 Only authorized personnel should be allowed to operate the instruments. The personnel must be aware of the possible danger which might occur while using the microscope. The Axio Scope.A1 is a precision tool which can easily be damaged or even destroyed when handled improperly.
  • Page 12 INTRODUCTION Carl Zeiss Safety Guidelines Axio Scope.A1 Never look into the light beam of the illumination equipment - with or without optical instruments. Not even if you just want to look at a specimen. Irreparable eye damage may occur! Do not hold any flammable or easily combustible materials around the light beam.

  • Page 13: Fig

    INTRODUCTION Axio Scope.A1 Safety Guidelines Carl Zeiss Caution signs on lower stand part for HAL 100- and HAL 50-illumination and on upper stand part FL-LED Fig. 1-1 Caution sign on lower stand part for HAL 100- and HAL 50-illumination Fig. 1-2...

  • Page 14: Warranty 1

    Only Carl Zeiss service personnel or personnel authorized especially by Carl Zeiss may perform repair work on the microscopes. If your instrument happens to malfunction please contact the Carl Zeiss Microscopy Service Department, Germany, (see p. 114) or the Carl Zeiss agency assigned to your country.

  • Page 15: Stand Variants 1

    INTRODUCTION Axio Scope.A1 Stand Variants Carl Zeiss Stand Variants The shipment includes five different upper stand parts, three lower stand parts and two stand columns which can be combined according to the following chart. Upper stand part Upper stand part...
  • Page 16 INTRODUCTION Carl Zeiss Stand Variants Axio Scope.A1 Upper stand Upper stand part Upper stand part Upper stand part Upper stand part Upper stand part Backlight FL/HBO FL-LED HAL 100/HBO HAL 100/HBO part with nosepiece with nosepiece with nosepiece with nosepiece 6x...
  • Page 17 INTRODUCTION Axio Scope.A1 Interface Diagram Carl Zeiss Interface Diagram The following figure shows a diagram of the interfaces of the microscope stand. The combination upper stand part FL/HBO and lower stand part with HAL 50 illumination serves as demonstration. The interfaces vary according to which upper or lower stand part has been chosen.
  • Page 18 INTRODUCTION Carl Zeiss Interface Diagram Axio Scope.A1 Fig. 1-3 Interface diagram (upper stand part FL/HBO and lower stand part with HAL 50 illumination) M60-2-0007 e 05/08...

  • Page 19: F Ig. 1-4 Summary Of The Controls And Functional Elements

    INTRODUCTION Axio Scope.A1 Summary of the Controls and Functional Elements Carl Zeiss Summary of the Controls and Functional Elements Legend of F ig. 1-4: 2 8 5 H 2 8 5 H Eyepieces Binocular tube part Field diaphragm (non removable or as slider)

  • Page 20: Fig

    INTRODUCTION Carl Zeiss Summary of the Controls and Functional Elements Axio Scope.A1 Fig. 1-4 Summary of the controls and functional elements M60-2-0007 e 05/08...

  • Page 21: Technical Manual 2

    Depending on the configuration of the microscope stand, they may be used with overhead or transmitted light only or with a combination of both. The Axio Scope.A1 may be used in typical biomedical application fields such as: − Medical exams in laboratories (research), hospitals, doctors‘ offices, −...

  • Page 22: Systems Overview 2

    TECHNICAL MANUAL Carl Zeiss Systems Overview Axio Scope.A1 Systems Overview M60-2-0007 e 05/08...
  • Page 23 TECHNICAL MANUAL Axio Scope.A1 Systems Overview Carl Zeiss M60-2-0007 e 05/08...
  • Page 24 TECHNICAL MANUAL Carl Zeiss Systems Overview Axio Scope.A1 M60-2-0007 e 05/08...
  • Page 25 TECHNICAL MANUAL Axio Scope.A1 Systems Overview Carl Zeiss M60-2-0007 e 05/08...
  • Page 26 TECHNICAL MANUAL Carl Zeiss Systems Overview Axio Scope.A1 M60-2-0007 e 05/08...

  • Page 27: Technical Data 2

    Technical Data Dimensions (width x depth x height) Microscope stand Axio Scope.A1 (no DL illumination) ..... approx. 240 mm x 340 mm x 365 mm Stand column Vario 380 mm ............approx. 460 mm x 390 mm x 465 mm Stand column Vario 560 mm ............

  • Page 28: Axio Scope.a1

    Microscope stand Axio Scope.A1 for LED illumination in transmitted light ... 2x T 3.15 A/H, 5x20 mm Microscope stand Axio Scope.A1 for HAL 50 illumination in transmitted light ..2x T 3.15 A/H, 5x20 mm Transformer mbq52ac-z for HBO 50...............100 V, 127 V: 2x T 4 A ..........................220 V - 240 V: 2x T 2.5 A...

  • Page 29: Setting Up The Microscope

    Carl Zeiss STARTUP The customer has the choice to either set up or mount the Axio Scope.A1 himself before putting it into operation, or have the Zeiss Customer Service do so for a fee. Please read the Safety Guidelines carefully before assembling and operating the microscope.

  • Page 30: Fig

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.2 Mounting the Upper Stand Part on the Stand Column If you are going to use a microscope stand consisting of an upper stand part and a stand column, please begin by assembling the upper stand part.

  • Page 31: F Ig. 3-3 Mounting The Binocular Tube

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.3 Mounting the Binocular Tube / Photo Tube All binocular tubes listed in the systems overview (chapter 2 .2) can be mounted on the microscope 2 9 4 H 2 9 4 H stand.

  • Page 32: Inserting An Eyepiece Graticule

    F ig. 3-5/4) and can thus be 3 0 5 H 3 0 5 H changed easily. Complete lens parts with glued-in graticules can be ordered from Zeiss. How to change a lens part: • Unscrew the lens part ( F ig.

  • Page 33: Mounting Objectives

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.5 Mounting Objectives • Lower the mechanical stage with the stage carrier to the bottom stop or lower the stage carrier with the dovetail. • Remove the dust protection caps ( F ig.

  • Page 34: Changing The Reflector Module In The Reflector Insert

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.6 Installing and Uninstalling "Push&Click" Modules in the Reflector Insert To install or uninstall the modules, the reflector insert (reflector turret 4- or 6-fold, reflector slider 2-fold) must be removed from the upper stand part.

  • Page 35: Mounting The Reflector Insert

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.7 Mounting the Reflector Insert • First remove the cover cap ( F ig. 3-9/1) by 3 2 2 H 3 2 2 H inserting the ball head screwdriver (SW 3 mm) into the slit ( F ig.

  • Page 36: Changing The Stable Mechanical Stage

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.8 Mounting the Mechanical Stage Depending on the type, the mechanical stages can either be stable or they can be rotated and centered. The shift range is 75 mm in X-direction and 50 mm in Y-direction.

  • Page 37: F Ig

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss b) How to mount the stage • Loosen the screw caps ( F ig. 3-11/2) of the spring box with approx. 3 turns. 3 3 8 H 3 3 8 H •...

  • Page 38: Adjusting Ergonometric Drive

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 (4) Removing and mounting the additional sleeves Both gear knobs for the stages with the order no. 432035-9030-000, 432035-9040-000 432035-9050-000 are equipped with additional sleeves for an even more sensitive adjustment of the object position.

  • Page 39: F Ig. 3-14 Adjusting The Frictional Momentum

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.9 Mechanical Stages with Friction Adjustment Drive length and friction for the X- and Y-adjustment can be adjusted individually on the standard mechanical stage (432035-9000-000) and the mechanical stage 432035-9070-000. (1) Adjusting the drive length on the stage...

  • Page 40: Adjusting The Connecting Rod Position For The Ergo Drive

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 (3) Adjusting the connecting rod position of the ergo drive The connecting rod position on the ergo drive of the mechanical stage 75x50 R (432035-9010) can be adjusted individually. • Remove the stage with the stage carrier from the stand (see chapter 3 .1.13) and put it aside...

  • Page 41: Mounting The Led Illuminator On The Condenser

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.10 Mounting the LED Illuminator for Transmitted Light BE CAREFUL, LED radiation! LED class 2M, do not look into the beam! The LED illuminator can only be inserted on the lower stand part for LED illumination.

  • Page 42: Mounting The Condenser Carrier

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 Guidelines for the use of the LED illuminator accessories: The slider with diffusion disk 10° emits more light for examining objects with the eyepieces. The slider with diffusion disk 80° emits a more homogeneous light for examining objects with the camera.

  • Page 43: Mounting The Condenser

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.12 Mounting the Condenser • Slide the stage carrier with the focusing drive to the upper stop. • Open the frontal optic (if switchable) on the condenser with the lever ( F ig.

  • Page 44: Mounting The Stage Carrier

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.13 Mounting the Stage Carrier • Screw the shoulder screw ( F ig. 3-20/3) into the 4 0 5 H 4 0 5 H appropriate opening: − Opening with mark 0: no adapter is mounted for specimen holder expansion F ig.

  • Page 45: Removing A Halogen Lamp Hal 50

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.14 Inserting the Halogen Lamp 12 V, 50 W • Switch off the microscope and pull its plug. Wait at least 15 min for the halogen lamp to cool down. • Remove the halogen lamp HAL 50 ( F ig.

  • Page 46: F Ig

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.15 Halogen Lamp HAL 100 The halogen lamp HAL 100 may serve as light source for transmitted light and reflected light procedures (except fluorescence light) as long as either the lower stand part or the upper stand part are equipped with the appropriate connection piece.

  • Page 47: Adjusting A Halogen Lamp Hal 100

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.15.2 Adjusting halogen lamp HAL 100 Rough setting • After loosening the clamping screw ( F ig. 3-23/1 4 2 8 H 4 2 8 H F ig. 3-23/7), remove the ready-to-operate...

  • Page 48: Changing A Halogen Lamp

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.15.3 Changing the halogen lamp 12 V, 100 W CAUTION Hot surface! DANGER OF BURNS! Always let the lamp case cool off for at least 15 min.! It is not necessary to remove the lamp case from the stand when replacing the halogen lamp.

  • Page 49: F Ig

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.16 Inserting the Adjustment Aid into the Upper Stand Part FL/HBO • Remove the cover ( F ig. 3-26/1) from the 4 4 7 H 4 4 7 H mounting aperture of the adjustment aid.

  • Page 50: F Ig

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 • Press the spring lever ( F ig. 3-28/5) on the 4 5 4 H 4 5 4 H heatsink up and pull the heatsink ( F ig. 3-28/1) 4 5 5 H 4 5 5 H off the inserted dummy or the used burner ( F ig.

  • Page 51: Mounting The Hbo

    Fig. 3-29 Mounting the HBO 50 3.1.17.3 Adjusting the HBO 50 illuminator If the Axio Scope.A1 is equipped with an adjustment aid (to be ordered separately), the HBO 50 can remain on the fluorescence reflected light illuminator when adjusting the burner.

  • Page 52: Mounting The Hbo 50

    STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 3.1.18 HBO 100 Illuminator 3.1.18.1 Inserting the mercury vapor short arc lamp HBO 103 W/2 For safety reasons, the illuminator HBO 100 and the mercury vapor short arc lamp HBO 103 W/2 are packed separately.

  • Page 53: Fig

    STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss 3.1.18.3 Adjusting the HBO 100 illuminator The HBO 100 illuminator is available in two variants (manual and automatic adjustment). The self-adjusting HBO 100 (423011-9901-000) adjusts automatically when the transformer is turned on.

  • Page 54: Hal 50 Illumination

    STARTUP Carl Zeiss Connection of the Power Supply Axio Scope.A1 Connection of the Power Supply 3.2.1 Lower Stand Part for LED and HAL 50 Illumination • Connect microscope’s power supply F ig. 3-35/2) with its power cord to an electrical 4 8 6 H 4 8 6 H outlet.

  • Page 55: Lower Stand Part For Hal 100 Illumination

    STARTUP Axio Scope.A1 Switching the Microscope and its Separate Illumination Fixtures on and off Carl Zeiss 3.2.3 Upper Stand Part for HAL 100/HBO, FL/HBO, FL-LED • Mount the halogen lamp HAL 100 to the stand (see chapter 3 .1.15.1). 4 9 2 H 4 9 2 H •...

  • Page 56: F Ig

    STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 Mounting Optional Components Make sure to unplug the stand and the external power supply units before you begin any mounting work. After finishing working the appropriate components must be put back into functional condition (see chapters 3 .1 to...

  • Page 57: Mounting The Intermediate Plate For Analyzer Slider 5

    STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss 3.4.2 Mounting the Intermediate Plate for Analyzer Slider • Loosen the hexagon socket screw ( F ig. 3-40/5) 5 1 2 H 5 1 2 H with the ball head screwdriver SW 3 and lift off the tube ( F ig.

  • Page 58: Mounting The Magnification Changer

    STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 3.4.4 Mounting Magnification Changer • If necessary, remove camera with adapter (or dust protection cover) from the camera port of the photo tube ( F ig. 3-41/2). 5 2 1 H 5 2 1 H •...

  • Page 59: F Ig

    – already low in Zeiss filter sets – even further. When mounting a filter without any orientation marks (arrow), we advise to proceed like this: Filters with reflecting, di-electrical coating are mounted so that the reflecting coating ( F ig.

  • Page 60: F Ig

    STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 3.4.6 Changing the Color Splitter in the Reflector Module FL P&C Be extremely careful when mounting the filters and the color splitter in order to avoid damaging and soiling the optical components.

  • Page 61: Labeling The Color Splitter

    STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss The reflecting (coated) side ( F ig. 3-46/3) 5 5 0 H 5 5 0 H of the color splitter has a tapered edge F ig. 3-46/1) or corner ( F ig. 3-46/2).

  • Page 62: Mounting The Overview Fixture

    STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 3.4.8 Mounting Centering Overview Fixture • If necessary, remove polarizer or filter holder from the condenser carrier. • Hold the overview fixture ( F ig. 3-48/4) parallel 5 6 7 H 5 6 7 H to the bottom of the condenser carrier F ig.

  • Page 63: Changing The Led Module

    STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss 3.4.9 Changing the LED Module in the Upper Stand Part FL-LED • Lift the covering caps ( F ig. 3-49/1) off the upper 5 7 6 H 5 7 6 H stand part.

  • Page 64: Inserting A Modulator Disk In The Condenser 0.9/1.25 H 6

    STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 3.4.10 Inserting a Modulator Disk in the Condenser 0.9/1.25 H • Remove the condenser ( F ig. 3-50/1) from the 5 8 0 H 5 8 0 H condenser carrier (see chapter 3 .1.12).

  • Page 65: Mounting The Slit-Diaphragm

    STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss 3.4.11 Changing the Diaphragm PlasDIC How to mount the slit-diaphragm (426715-9000- 000): • Turn the Ph-diaphragm which needs to be changed into free aperture modulator disk. • Screw the modulator disk’s centering screws F ig.

  • Page 66: Changing The Filter In The Filter Wheel Transmitted Light

    STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 3.4.13 Changing the Filter in the Filter Wheel Transmitted Light • Remove the stage carrier (see chapter 3 .1.11). 5 9 9 H 5 9 9 H • Unscrew three screws ( F ig.

  • Page 67: Default Setting Of The Microscope 6

    STARTUP Axio Scope.A1 Default Setting of the Microscope Carl Zeiss Default Setting of the Microscope 3.5.1 Adjusting the Eyepiece Distance (Distance of the Pupils) on the Binocular Tube • Adjust the eyepiece distance (distance of the pupils) according to the individual distance of the operator’s eyes (...

  • Page 68: Correction Of Vision Defects With The Eyepiece Graticules 6

    STARTUP Carl Zeiss Default Setting of the Microscope Axio Scope.A1 3.5.3 Correction of Vision Defects with the Eyepiece Graticules If you are going to use an eyepiece graticule you will need two adjustable graticules, e.g. PL 10x/23 Br. foc., in order to correct different vision defects of different operators.

  • Page 69: Operation 6

    All available condensers (except special condensers like dark-field condensers) can be used for the transmitted light/bright-field method. (3) Adjusting the transmitted light/bright-field according to KÖHLER − The Axio Scope.A1 has been put into operation appropriately (chapter 3 ). 6 0 9 H 6 0 9 H −...

  • Page 70: Fig

    OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 • Center the field diaphragm image with both centering screws ( F ig. 4-1/4) on the condenser 6 2 0 H 6 2 0 H carrier ( F ig. 4-1/C). Open the field diaphragm...

  • Page 71: Fig

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss (4) Adjusting vertical stop condenser carrier • Loosen the setscrew ( F ig. 4-2/1) on the vertical 6 2 5 H 6 2 5 H stop with the ball-head screwdriver SW 3.

  • Page 72: Adjusting The Transmitted Light/Dark-Field According To Köhler 7

    OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 4.1.2 Adjusting the Transmitted Light/Dark-Field According to KÖHLER (1) General principle of operation Uncolored biological samples, like bacteria or living cell cultures, are sometimes hard to see in the transmitted light/bright-field due to their translucence. Yet it is completely different when you examine these samples with the transmitted light/dark-field method.

  • Page 73: Fig

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss • Replace the eyepiece into the tube. • Lighter areas which might still be visible in the field of view are reduced when the dark-field condenser is adjusted correctly and delicately.

  • Page 74: Adjusting The Transmitted Light/Phase-Contrast 7

    OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 4.1.3 Adjusting the Transmitted Light/Phase-Contrast (1) General principle of operation The phase-contrast method is ideal for examining thin uncolored samples, e.g. culture cells. Generally the human eye cannot detect phase differences (variations in refractive index or thickness) within the different cell components.

  • Page 75: Fig

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss • If the overlap is not exact ( F ig. 4-5/A), the 6 3 2 H 6 3 2 H lighter annular diaphragm must be recentered with the aid of two hexagon screwdrivers SW 1.5 (...

  • Page 76: Adjusting The Transmitted Light/Differential Interference Contrast (Dic) 7

    OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 4.1.4 Adjusting the Transmitted Light/Differential Interference Contrast (DIC) (1) General principle of operation The transmitted light/DIC method allows for a high-contrast vivid display of transparent sample details. The light is linearly polarized by a polarizer and is separated into two beams in a birefringent prism. The two beams penetrate two parts of the sample which are closely separated.

  • Page 77: Fig

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss DIC slider Knurled screw Objective on the nosepiece Condenser with DIC prism Compensator λ Analyzer module Reflector turret Fig. 4-6 Components for the transmitted light/DIC method M60-2-0007 e 05/08...

  • Page 78: Adjusting Plasdic-Contrast In Transmitted Light 7

    OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 4.1.5 Adjusting PlasDIC-Contrast in Transmitted Light (1) General principle of operation PlasDIC can be used independently from the material of the specimen holder. The contrast method gives a relief-like image and is especially well suited for thicker objects. The contrast is adjustable.

  • Page 79: Components For Transmitted Light Polarization

    Because of the crossed polarizers the field of view now appears dark. If an analyzer slider is going to be used for working with the Axio Scope.A1, either the reflector module H P&C or an empty position must be swung in on the reflector turret/slider.

  • Page 80: F Ig. 4-8 Determination Of The Polarization Direction N

    Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 • Bring the object to be examined into the field of view and turn it with the rotary stage. Normally, birefringent (anisotropic) objects will now show the same color and intensity variations as described above during rotation between crossed polarizers.

  • Page 81: Diagram Of The Color Tables According To Michel-Lévy

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss (3) Adjusting the microscope • Adjust the microscope as described in chapter 4 .1.1 (3) for transmitted light/bright-field. Make sure 6 5 0 H 6 5 0 H the eye distance is adjusted correctly on the binocular tube (see chapter 3 .5.1).
  • Page 82 OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 The phase difference results from the interference of the polarization of the sample and the polarization of the compensator λ. The larger phase difference occurs when the polarization direction of the sample or the absolute or relative largest index of refraction (n γ...

  • Page 83: F Ig

    (2) Instrumentation − Axio Scope.A1 with adjusted halogen lamp HAL 100 mounted on the reflected light barrel − Reflector module bright-field ACR P&C for reflected light in the reflector turret /slider − Upper stand part HAL 100/HBO 6x HD, DIC with pre-installed aperture and field diaphragm or...
  • Page 84 OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 • Adjust the knurled knob on the field diaphragm ( F ig. 4-11/4) so that the field diaphragm becomes 6 6 3 H 6 6 3 H visible in the field of view ( F ig.

  • Page 85: Adjusting The Microscope In The Reflected Light/Bright-Field

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss Centering screw of the aperture diaphragm Knurled button of the aperture diaphragm Centering screw of the field diaphragm Knurled button of the field diaphragm Focusing drive Nosepiece Fig. 4-11 Adjusting the microscope in the reflected light/bright-field...

  • Page 86: Adjusting The Reflected Light/Dark-Field 8

    (2) Instrumentation − Axio Scope.A1 with adjusted halogen lamp HAL 100 mounted on the reflected light barrel − Objectives Epiplan-Neofluar, EC Epiplan-Neofluar, Epiplan with the additional label "HD" − Reflector module dark-field ACR P&C for reflected light in the reflector turret/slider (3) Adjusting the reflected light/dark-field •...

  • Page 87: Adjusting Reflected Light Dic And Reflected Light C-Dic 8

    (2) Instrumentation − Axio Scope.A1 with adjusted halogen lamp HAL 100 mounted on the reflected light barrel − Objectives EC Epiplan-Neofluar, Epiplan with the additional label "DIC" or "Pol" − DIC slider, fitted to the objective engraved on the top surface of the slider with amplification and aperture or C-DIC slider 6x20 (in connection with the reflector module C DIC P&C, compensator...

  • Page 88: Tic Slider 6X20

    (2) Instrumentation − Axio Scope.A1 with adjusted halogen lamp HAL 100 mounted on the reflected light barrel − Objectives EC Epiplan-Neofluar, Epiplan with additional label "DIC"...
  • Page 89 OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss The values a and b (see F ig. 4-14) are determined 6 8 4 H 6 8 4 H with the aid of the eyepiece graticule micrometer or with the micrometer eyepiece.
  • Page 90 OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 Attention: • If the step and its surrounding are made from different materials, the phase jumps characteristic for the material have to be considered. For all non-conducting materials the phase jump is 180°, and for all semi-conductors only slightly different from 180°.
  • Page 91 (2) Instrumentation − Axio Scope.A1 with mounted and adjusted halogen lamp HAL 100 − Rotary stage Pol − Objectives Epiplan-Neofluar Pol, EC Epiplan-Neofluar Pol, Epiplan Pol −...

  • Page 92: Components For Reflected Light Polarization

    OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 A sample is bireflective when the sample details show differences in brightness and color which change when the stage rotates. For samples with low bireflexion we advise using the analyzer with Lambda plate, rotatable.

  • Page 93: Adjusting Reflected Light Fluorescence 9

    OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss 4.1.12 Adjusting Reflected Light Fluorescence (1) General principle of operation The reflected light fluorescence method is used to show fluorescent substances in typical fluorescence colors in high contrast. The light originating from a high-performance illuminator in a reflected light fluorescence microscope passes through a heat protection filter onto an excitation filter (bandpass).
  • Page 94 OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 • Switch on the lamp HBO 100 or HBO 50 F ig. 4-16/1) and let it warm up to operational 6 9 1 H 6 9 1 H temperature for about 15 min.

  • Page 95: F Ig

    OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss Operating and Functional Elements of Optional Components 4.2.1 Lower Stand Part for LED Transmitted Light Illumination LED illumination (mounted on condenser carrier) Light intensity control (both sides) Cord guide to the connector socket for...

  • Page 96: F Ig

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 4.2.2 Lower Stand Part with HAL 50 Illumination Field diaphragm Power input with fuse compartment Filter wheel 6-fold (both sides) Halogen lamp HAL 50 Focusing drive – fine adjustment (both sides) Release tractor for vertical stop of focusing drive Focusing drive –...

  • Page 97: F Ig

    OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss 4.2.3 Lower Stand Part for HAL 100 Illumination Field diaphragm Connecting nozzle for halogen lamp HAL 100 Filter wheel 6-fold (both sides) Release tractor for vertical stop of focusing drive Focusing drive –...

  • Page 98: F Ig

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 4.2.4 Stand Column Vario 380 mm or 560 mm with Gear Box Hand wheel for vertical adjustment of gear box of stand column, Gear box Vario ready for upper stand part Focusing drive –...

  • Page 99: F Ig. 4-21 Upper Stand Part Transmitted Light

    OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss 4.2.5 Upper Stand Part Transmitted Light Nosepiece 6x bright-field Reflector insert (exchangeable) Fig. 4-21 Upper stand part transmitted light 4.2.6 Upper Stand Part FL/HBO Field diaphragm (centerable) Port for adjustment aid...

  • Page 100: F Ig. 4-23 Upper Stand Part Fl-Led

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 4.2.7 Upper Stand Part FL-LED Reflector turret 4-fold (fixed) Control wheel for illumination intensity and on/off switch of LED illumination Pilot lamp LED illumination on/off Nosepiece 3x bright-field, 3x DIC The upper stand part FL-LED comes equipped with an integrated LED illumination.

  • Page 101: F Ig. 4-24 Upper Stand Part Hal 100/Hbo

    OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss 4.2.8 Upper Stand Part HAL 100/HBO Field diaphragm (centerable) Aperture diaphragm (centerable) Compartment for filter slider A Connecting piece for illumination unit Diffusing panel for reflected light Nosepiece 6x bright- and dark-field Reflector insert (exchangeable) Fig.

  • Page 102: F Ig. 4-26 Sideport Iscp T60N Left

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 4.2.10 Sideport ISCP T60N Left The sideport ISCP T60N left is equipped with two switch positions which can be activated alternately with the slider. Push the slider to the right hand position and activate the eyepiece observation (vis).

  • Page 103: F Ig. 4-27 Reflector Slider 2-Fold

    OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss 4.2.11 Reflector Slider 2-fold The reflector slider 2-fold is equipped with two individually loadable reflector positions P&C which you can slide into the beam path. Slider Fig. 4-27 Reflector slider 2-fold 4.2.12...

  • Page 104: Condenser 0.9/1.25 H With Modulator Disk

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 Condenser 0.9/1.25 H condenser 0.9/1.25 H F ig. 4-29/1) with 7 0 0 H 7 0 0 H aperture diaphragm ( F ig. 4-29/2) is equipped to 7 0 1 H 7 0 1 H your choice with a modulator disk ( F ig.
  • Page 105 100 % to the photo port. The camera port may be loaded with a maximum weight of 2.5 kg (camera plus cable). For a fee, the Carl Zeiss service personnel will equip your instrument with a higher maximum load, if needed.

  • Page 106: Binocular Photo Tube

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 Binocular photo tube (100/100) The photo port ( F ig. 4-32/1) of the binocular tube 7 1 0 H 7 1 0 H may serve for mounting a reflex camera, a microscope camera or a video camera with the aid of appropriate adapters.

  • Page 107: Mechanical Stage With Object Holder

    OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss Mechanical stage 75x50 − Mechanical stage ( F ig. 4-34/2) with object 7 1 7 H 7 1 7 H holder ( F ig. 4-34/1) for holding, positioning 7 1 8 H 7 1 8 H and fixating the samples.

  • Page 108: F Ig. 4-36 Diaphragm Slider 14X40

    OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 Diaphragm slider 14x40 mm for aperture and field diaphragm − You will need diaphragm slider 14x40 mm each ( F ig. 4-36/4, inserted in the 7 2 3 H 7 2 3 H...

  • Page 109: Maintenance, Changing Of Fuses And Service 1

    Carl Zeiss MAINTENANCE, CHANGING OF FUSES AND SERVICE Maintenance Maintenance of the Axio Scope.A1 is limited to the following activities: • Turn the instrument off after each use and cover it with the appropriate protection cover (dust and humidity protection).

  • Page 110: Fig

    MAINTENANCE, CHANGING OF FUSES AND SERVICE Carl Zeiss Technical Service Axio Scope.A1 Technical Service 5.2.1 Check-Up • Be sure to adhere to the required voltage (e.g. on the transformer HBO 100 W). • Check the power cable and the power plug for damages.

  • Page 111: Trouble Shooting 1

    MAINTENANCE, CHANGING OF FUSES AND SERVICE Axio Scope.A1 Trouble Shooting Carl Zeiss Trouble Shooting Problem Cause Fault clearance Shading or brightness irregularities Push rod/gear knob vis/fot on the Bring the push rod/gear knob vis/fot in the field of view of the...

  • Page 112: Service 1

    MAINTENANCE, CHANGING OF FUSES AND SERVICE Carl Zeiss Trouble Shooting Axio Scope.A1 Problem Cause Fault clearance Asymmetric image sharpness, e.g. Condenser is not adjusted properly. Re-adjust the condenser, one side is sharp, one is side see p. 6 9 ff.
  • Page 113 MAINTENANCE, CHANGING OF FUSES AND SERVICE Axio Scope.A1 Trouble Shooting Carl Zeiss Problem Cause Fault clearance Halogen lamp 12 V, 50 W does Power plug is not plugged into the Insert the plug into the outlet. Make not glow although the switch in outlet.

  • Page 114: Carl Zeiss

    In order to guarantee that your microscope remains in optimal and faultless working order for the longest possible time, we recommend that you conclude a service / maintenance contract with Carl Zeiss. If you want to place an additional order or if a service incident occurs, please contact your Carl Zeiss representative.

  • Page 115: Annex

    ANNEX Axio Scope.A1 List of Abbreviations Carl Zeiss ANNEX List of Abbreviations Alternating Current Automatic Component Recognition A-Plan Achromatic objectives with improved flatness of field (ICS optical products) Suitable for spectacle wearers Charge-Coupled Device Canadian Standards Association C-DIC Differential Interference Contrast in circular polarized light...
  • Page 116 ANNEX Carl Zeiss List of Abbreviations Axio Scope.A1 Right-hand (gear knob on the right-hand side of the mechanical stage) Single Lens Reflex Wrench Size slow (type of fuse) Total Interference Contrast in circular polarized light Underwriter Laboratories ultra-violet visual W-PL...

  • Page 117: Subject Index 1

    ANNEX Axio Scope.A1 Subject Index Carl Zeiss Subject Index page Achromatic illumination adapter ......................17 Adapter ..............................17 Adjustment aid ..........................17, 19 Analyzer ......................57, 76, 77, 78, 79, 80, 81, 92 Analyzer module............................79 Analyzer slider ..........................57, 79 Aperture diaphragm ......................19, 70, 94, 104 Binocular photo tube ...........................106...
  • Page 118 ANNEX Carl Zeiss Subject Index Axio Scope.A1 Field diaphragm.......................19, 69, 75, 94, 96, 97 Filter..............................58, 66 Filter slider.............................93 Filter slider reflected light.........................17, 19 Filter slider transmitted light......................17, 19 Filter wheel..........................19, 66, 96, 97 Filter wheel transmitted light .........................17 Fine adjustment......................19, 95, 96, 97, 98 Fluorescence............................93...

  • Page 119: Property Rights 1

    ANNEX Axio Scope.A1 Subject Index Carl Zeiss Nosepiece........................19, 33, 69, 83, 104 Object holder............................107 Objectives............................33, 37 On/off switch..........................19, 95, 96 Operating and functional elements ......................95 Operation ..............................69 Overview fixture ..........................62, 107 Phase-contrast ............................74 PlasDIC-contrast ............................78 Polarization............................79, 91 Polarization direction ..........................80 Polarizer ............................61, 79...
  • Page 120 ANNEX Carl Zeiss Subject Index Axio Scope.A1 Transmitted light .....................69, 72, 74, 76, 78, 79 Transmitted light lamp...........................19 Trouble shooting ..........................111 Tube..............................17, 31 Tube lens turret .............................57 Universal condenser..........................69 Unpacking.............................29 Upper stand part ..........................17, 56 Vertical adjustment of gear box ......................98 Vertical stop on focusing drive .......................19...
  • Page 121 ANNEX Axio Scope.A1 Property Rights Carl Zeiss Property Rights The instruments, their components and the methods described in this manual are protected by the following patents: − US 6154282 − US 6392796 − US 5015082 − US 6595661 − DE 4322015 −...
  • Page 123 Click below to find more Mipaper at www.lcis.com.tw Mipaper at www.lcis.com.tw...

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