Microscopy In A Few Steps - Zeiss Axioskop 2 plus Operating Manual

Axioskop 2 plus
Axioskop 2 mot plus
Microscopy in a few steps using the example of "Setting of transmitted-light brightfield
according to KÖHLER"
☞
Please read the instructions regarding instrument safety and the Description (Chapter 1) and
Start Up (Chapter 2) chapters before using the Axioskop 2 plus and/or Axioskop 2 mot plus
microscopes for the first time.
(1) As described in chapter 2, the microscope is ready for operation and switched on according to
section 3.3.
(2) Place a high-contrast specimen on the mechanical stage (cover slip pointing upwards).
(3) Swing in 10x objective on the nosepiece and focus on the specimen; if possible, always move
the specimen away from the objective!
(4) Move condenser - e.g. universal condenser 0.9 - (in brightfield position, front lens switched in)
to the upper stop position via the condenser drive. The height adjustment must be set in such
a way that the specimen is not touched by the condenser.
(5) Close luminous-field diaphragm until it is visible in the field of view, even if not in focus
(Fig. 0-1/A).
(6) Lower universal condenser 0.9 until the edge of the luminous-field diaphragm appears in focus
(Fig. 0-1/B).
(7) Center luminous-field diaphragm using centering screws on the condenser carrier (Fig. 0-1/C)
and open it until its edge just disappears behind the field of view (Fig. 0-1/D).
(8) To set the aperture diaphragm (contrast), remove one eyepiece from the binocular tube and
set the aperture diaphragm to approx. 2/3 of the diameter of the objective exit pupil
(Fig. 0-1/E). Optimum contrast setting is dependent on the respective specimen.
(9) Insert eyepiece again and, if required, refocus on the specimen via fine drive.
(10) After each objective change, field size and objective aperture change, which makes it
necessary to repeat steps (5) to (9).
(11) For objective magnifications between 1.25x and 5x, the front lens 0.9 of the universal
condenser must be swung out and the aperture diaphragm opened entirely.
Fig. 0-1 Diaphragm settings in transmitted-
light brightfield according to
KÖHLER
B 40-075 e 10/03
INTRODUCTION

Microscopy in a few steps...

Carl Zeiss
0-7