Appendix A Specifications
A.4
Reader Specifications
Photometric Range
Spectral Range
Precision
Accuracy
Linearity
Read Time
A.5
Washer Specifications
Manifold Configuration
Programmable Volumes
Wash Containers
Clean Container
Waste Container
Residual Wash Volume
Dispense Precision
A.6
Incubator Specifications
Number of Incubators
Temperature Range
Temperature Accuracy
Temperature Uniformity across plate
Shaking
146
0.000 to 3.500 OD
405 to 690 nm
± 0.010 OD at 0.000 – 0.500 OD
< 1% CV between 0.501 OD and 2.0 OD
< 1.5% CV between 2.01 and 3.0 OD
± 0.010 OD or 2.5% (whichever is greater)
between 0 and 3.0 OD
± 1% from 0.000 to 2.500 OD
≤ 1.5% at >2.500 OD
<30 seconds, single wavelength
<50 seconds, dual wavelength
8-way wash head
50-999 µL
4 wash bottles at 3.0 L, with quantitative level
sensing
1 wash bottle at 3.0 L, with quantitative level
sensing
10 L, with quantitative level sensing
< 3µL per well with dual-axis sweep in a flat
bottom well
≤ 5% CV (with 300 µL in a 96 well plate)
Up to 12 (Ambient) Up to 6 (Elevated)
o
o
RT+4
C to 45
C (Elevated Incubator)
o
RT+4
C (Ambient Incubator)
±1
o
C
±1
o
C
14 Hz periodic or continuous
Agility System Operator's Manual