Thermo Scientific Dionex Aquion RFIC Operator's Manual page 107

Thermo Scientific
4. Draw the syringe back to begin pulling eluent through the flow path. (It may take several
syringe draws to remove all air or previous eluent from the tubing.)
Note If the vacuum degas assembly is installed (see
(Optional)" on page
5. After priming the lines thoroughly, close the priming valve. Do not overtighten the valve.
To prime the pump heads

Before priming the pump heads, the eluent lines must be primed (see
1. Verify that the priming valve on the primary pump head is closed (see
Figure 45. Priming the pump heads
Secondary
Pump Head
Waste Valve
(open)
Priming Valve
2. Open the waste valve on the secondary pump head by turning the knob one-quarter to
one-half turn counterclockwise (see
path to waste and eliminates backpressure.)
3. On the Chromeleon ePanel Set, click the Pump_ECD tab.
4. Under Pump_ECD, click the Prime button.
5. When Chromeleon displays a message reminding you that the waste valve is open, click
OK. The pump will begin pumping at about 3 mL/min.
6. Monitor the pump waste line for air bubbles. When no air bubbles are visible in the waste
line, close the waste valve. Do not overtighten the waste valve.
19), draw out an additional 20 mL of eluent.
Primary
Pump Head
(close)
Figure 45). (Opening the valve directs the eluent flow
Priming the Pump (Standard Procedure)
"Vacuum Degas Assembly
page 90).
Figure 45).
Dionex Aquion RFIC Operator's Manual
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Service
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