GE AKTAprime plus Operating Instructions Manual page 35

6 Choose to wash the A2–A8 inlet tubing that is used by pressing OK at those cursor
7 Scroll to OK and press the OK button.
8 Press OK to start the method.
9 When the method is finished, replace the first collection tube. It will contain a small
Purging the pump and inlet tubing
If there are large amounts of air in the tubing or if you suspect air in the pump, use the
Purge kit to purge the flow path. Air bubbles that still are trapped in the pump (causing
increased pulsation) can be removed by flushing 100% ethanol through the pump. These
two procedures are described in the following two sections.
Purging the flow path using the Purge kit:
1 Remove the stop plug from the pump.
2 Connect the Purge kit to the pump.
3 Put the used inlet tubing in the appropriate buffers.
4 Run the pump at 0.1 ml/min.
Filling inlet tubing A1–A8:
1 Go to Set Buffer Valve using the arrow buttons.
2 Set the chosen A inlet and press OK. The valve switches to the selected port.
3 Draw buffer with the purge syringe until liquid enters the syringe.
4 Repeat step 1–3 until all chosen A inlet tubing is filled.
Filling inlet tubing B:
1 Go to Set Concentration %B and set the concentration to 100%.
ÄKTAprime plus Operating Instructions 28-9597-89 AB
positions. A1 and B will always be washed.
Note:
At delivery, only A1 and B are installed.
amount of water after the system wash.
4 Operation
4.3 Preparations before start
35