GE AKTAprime plus Operating Instructions Manual page 36

4 Operation
4.3 Preparations before start
2 Press OK. The switch valve turns to the inlet B port.
3 Draw buffer with the purge syringe until liquid enters the syringe.
4 Replace the purge tubing with the stop plug.
5 Stop the pump by pressing end and then OK.
Flushing the pump with 100% ethanol:
1 Put inlet tubing A1 in deionized water.
2 Run the pump at 40 ml/min for 1 min and press pause/cont.
3 Move inlet tubing A1 to 100% ethanol
4 Press pause/cont, run the pump for 10–20 s and press pause/cont.
5 Set the flow rate to 5 ml/min using the arrow buttons.
6 Press pause/cont, run the pump for at least 30 s and press pause/cont.
7 Move inlet tubing A1 to deionized water.
8 Press pause/cont and run the pump for 1 min.
9 Finish by pressing end and then OK.
Preparing the tubing and column
1 Put inlet tubing A1 in the binding buffer.
2 Put inlet tubing B in the elution buffer.
3 Put the three waste capillaries (brown color) from port 4 and 5 on the injection valve
4 Connect a column, for example a HisTrap™ HP 1 ml column, between port 1 on the
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and port NO on the fraction collector valve in waste.
injection valve and the upper port of the UV flow cell. Use a suitable length of PEEK
tubing and 1/16" male connectors.
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ÄKTAprime plus Operating Instructions 28-9597-89 AB