GE AKTAprime plus Operating Instructions Manual page 52

6 Troubleshooting
6.2 Conductivity curve problems
Error symptom
Waves on the gradi-
ent
Ghost peaks appear
in the gradient profile
Unlinear gradients or
slow response to %B
changes
52
Possible cause
Incorrect pump func-
tion
Dirty mixing chamber
Insufficient mixing
chamber volume
Incorrect motor func-
tion
Air in the flow cell
Dirty tubing
Incorrect mixer vol-
ume
ÄKTAprime plus Operating Instructions 28-9597-89 AB
Corrective action
Check that the pump is operating
and is programmed correctly.
Check that the mixing chamber is
free from dirt or particles.
Change to a larger mixing cham-
ber volume if necessary.
Check the motor operation. Place
a hand on the mixer and start it by
starting the pump at a low flow
rate. You should both hear and feel
the mixer motor and stirrer when
they are spinning.
Check for loose tubing connec-
tions. Use the flow restrictor.
Wash the tubing and check pump
is operating properly.
Change to smaller mixer volume.