Table of Contents
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Table of Contents
Related Manuals for GE Monitor UV-900
Summary of Contents for GE Monitor UV-900
- Page 1 GE Healthcare Monitor UV-900 User Manual ÄKTA ™...
- Page 3 I m p o r t a n t u s e r i n f o r m a t i o n A l l u s e r s m u s t r e a d t h i s e n t i r e m a n u a l t o f u l l y u n d e r - s t a n d t h e s a f e u s e o f M o n i t o r U V - 9 0 0 .
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Page 5: Table Of Contents
Contents Introduction General ....................7 Safety ....................8 Installation Unpacking ..................9 General precautions ..............9 Installing the flow cell ..............10 2.3.1 Fixing the flow cell ..............10 2.3.2 Connecting the optical fibers ..........11 Connecting electrical signal cables ........12 2.4.1 Connecting to recorder (if used).......... - Page 6 Contents Cleaning-in-place .................25 Checking the instrument ............26 4.3.1 Lamp intensity ................26 4.3.2 Lamp on–time ................26 4.3.3 Flip time..................26 4.3.4 UV flow cell ...................26 Cleaning the flow cell and optical fiber connectors ..27 4.4.1 Cleaning the flow cell...............27 4.4.2 Cleaning the optical fiber connectors .......27 4.4.3 Instrument housing..............27 ..............Cleaning the system 27...
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Page 7: Introduction
Introduction Introduction General M o n i t o r U V - 9 0 0 i s a U V - a b s o r p t i o n m o n i t o r f o r u s e i n l i q u i d c h r o m a t o g r a p h y . M o n i t o r U V - 9 0 0 f e a t u r e s : •... -
Page 8: Safety
Introduction Safety M o n i t o r U V - 9 0 0 i s i n t e n d e d f o r l a b o r a t o r y u s e o n l y , n o t f o r I M P O R T A N T ! c l i n i c a l o r i n v i t r o u s e , o r f o r d i a g n o s t i c p u r p o s e s . -
Page 9: Installation
Installation Installation Unpacking U n p a c k t h e i n s t r u m e n t a n d c h e c k t h e i t e m s a g a i n s t t h e s u p p l i e d p a c k i n g l i s t . I n s p e c t t h e i t e m s f o r o b v i o u s d a m a g e w h i c h m a y h a v e o c c u r r e d d u r i n g t r a n s p o r t a t i o n •... -
Page 10: Installing The Flow Cell
Installation Installing the flow cell 2.3.1 Fixing the flow cell H a n d l e t h e o p t i c a l f i b e r s w i t h c a r e , d o n o t b e n d e x c e s s i v e l y . -
Page 11: Connecting The Optical Fibers
Installation T o i m p r o v e t h e a c c e s s t o t h e t u b i n g c o n n e c t i o n s , t h e f l o w c e l l c a n a l t e r n a t i v e l y b e p o s i t i o n e d w i t h t h e t u b i n g c o n n e c t i o n s . -
Page 12: Connecting Electrical Signal Cables
Installation Connecting electrical signal cables T h e s o c k e t s f o r e l e c t r i c a l s i g n a l s a r e l o c a t e d o n t h e b a c k p a n e l . Analogue out Remote UniNet 1 Mains... -
Page 13: Connecting To Supply Voltage
Installation C o n n e c t t w o U n i N e t c a b l e s t o t h e U n i N e t - 1 c o n n e c t o r s . T h e i n s t r u m e n t c a n b e c o n n e c t e d i n s e r i e s a n y w h e r e i n t h e c h a i n b e t w e e n t h e P C a n d t h e t e r m i n a t i o n p l u g . -
Page 14: Mounting The Cell Holder Cover
Installation Mounting the cell holder cover T h e c o v e r i s a s i m p l e p u s h f i t o n t o t h e c e l l h o l d e r . T w o s m a l l l u g s o n t h e c o v e r l o c a t e i n h o l e s a t t h e f r o n t a n d r e a r o f t h e c e l l h o l d e r T h e c o v e r i s t h e n l o w e r e d o v e r t h e c e l l h o l d e r . -
Page 15: Operation
Operation Operation On/off 1 S w i t c h o n t h e i n s t r u m e n t a t t h e m a i n s s w i t c h o n t h e r e a r p a n e l . Selftest Please wait... -
Page 16: Return To Main Menu
Operation I f a m e n u h a s s u b l e v e l s , t h e s u b m e n u i s d i s p l a y e d b y p r e s s i n g t h e O K b u t t o n . -
Page 17: Main Menu Overview
Operation Main menu overview M o d e c h a n g i n g m e n u ( m o d e m e n u ) . T h e m e n u i s a c c e s s e d f r o m a l l p o s i t i o n s b y Press OK to p r e s s i n g t h e b u t t o n r e p e a t e d l y . -
Page 18: Reading Absorbance Values
Operation Reading absorbance values T h e m a i n o p e r a t i n g m e n u s h o w s t h e a b s o r b a n c e v a l u e s f o r u p t o 3 a c t i v e λ1(215) 1.123 AU λ2(254) -
Page 19: Autozero
Operation Autozero T h e a u t o z e r o f u n c t i o n s e t s t h e d e t e c t e d a b s o r b a n c e t o z e r o w h e n t h e O K b u t t o n i s p r e s s e d . -
Page 20: Using An External Chart Recorder
Operation Using an external chart recorder 3.9.1 Setting range and zero T h e e x t e r n a l c h a r t r e c o r d e r o u t p u t f r o m t h e i n s t r u m e n t i s a l w a y s 0 – 1 V , b u t t h e a b s o r b a n c e v a l u e f o r f u l l s c a l e d e f l e c t i o n a n d t h e Z e r o A b s o r b a n c e l e v e l o n t h e r e c o r d e r c a n b e s e t . -
Page 21: Filtering Noise
Operation 3.10 Filtering noise T o f i l t e r t h e n o i s e i n t h e U V - s i g n a l , a m o v i n g a v e r a g e f i l t e r i s u s e d . T h e a v e r a g i n g t i m e i s t h e t i m e i n t e r v a l u s e d f o r c a l c u l a t i n g t h e m o v i n g a v e r a g e o f t h e a b s o r b a n c e s i g n a l . -
Page 22: Uv Cell Calibration
Operation 3.11 UV cell calibration T h e p a t h l e n g t h i n t h e U V f l o w c e l l m i g h t d i f f e r f r o m t h e n o m i n a l l e n g t h ( 1 , 2 , 5 r e s p e c t i v e 1 0 m m ) w h i c h i n t e r f e r e s t h e c a l c u l a t i o n o f t h e p r o t e i n c o n c e n t r a t i o n i n t h e e l u a t e . - Page 23 Operation 5 O p e n t h e U V - 9 0 0 C e l l C a l i b r . E x c e l - f i l e o n t h e c o m p u t e r . 6 T h e s o l u t i o n b o t t l e s a r e m a r k e d w i t h t h e c o n c e n t r a t i o n v a l u e a n d t h e r e f e r e n c e a b s o r b a n c e v a l u e f o r e a c h s o l u t i o n .
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Page 24: Changing Flow Cell
Operation 7 R e m o v e t h e s y r i n g e . 8 I n j e c t t h e o t h e r f o u r t e s t s o l u t i o n s i n i n c r e a s i n g c o n c e n t r a t i o n o r d e r . U s e a n e w s y r i n g e f o r e a c h s o l u t i o n . -
Page 25: Periodic Maintenance
Maintenance Maintenance A l w a y s d i s c o n n e c t t h e p o w e r s u p p l y b e f o r e a t t e m p t i n g t o W A R N I N G ! r e p l a c e a n y i t e m o n t h e s y s t e m d u r i n g m a i n t e n a n c e . -
Page 26: Checking The Instrument
Maintenance Checking the instrument 4.3.1 Lamp intensity Check Lamp Intensity 1 S e l e c t m e n u C h e c k , p r e s s O K . 210nm 85% 300nm 95% 2 S e l e c t m e n u C h e c k L a m p I n t e n s i t y . I f t h e l a m p i n t e n s i t y i s <... -
Page 27: Cleaning The Flow Cell And Optical Fiber Connectors
Maintenance Cleaning the flow cell and optical fiber connectors A c l e a n f l o w c e l l a n d o p t i c a l c o n n e c t o r s a r e e s s e n t i a l f o r e n s u r i n g t h e c o r r e c t o p e r a t i o n o f t h e U V - m o n i t o r . -
Page 28: Recycling
Maintenance Recycling T h i s s y m b o l i n d i c a t e s t h a t t h e w a s t e o f e l e c t r i c a l a n d e l e c t r o n i c e q u i p m e n t m u s t n o t b e d i s p o s e d a s u n s o r t e d m u n i c i p a l w a s t e a n d m u s t b e c o l l e c t e d s e p a r a t e l y . -
Page 29: Trouble-Shooting
W h e n c o n t a c t i n g G E H e a l t h c a r e f o r s u p p o r t , s t a t e t h e p r o g r a m v e r s i o n o f t h e Monitor UV-900 V1.06... -
Page 30: Error Messages
Trouble-shooting Error messages I f t h e s u g g e s t e d a c t i o n s d o n o t c o r r e c t t h e f a u l t , c a l l G E H e a l t h c a r e . M e s s a g e A c t i o n Sample fiber failure... -
Page 31: Reference Information
Reference information Reference information Description Instrument T h e M o n i t o r U V - 9 0 0 i s a U V - a b s o r p t i o n m o n i t o r w i t h v a r i a b l e w a v e l e n g t h . T h e i n s t r u m e n t c o n t a i n s n o i n t e r n a l u s e r r e p l a c e a b l e i t e m s . -
Page 32: F L O W C E L
Reference information Flow cell T h e o p t i c a l p a t h l e n g t h o f t h e f l o w c e l l i s 2 m m a n d 1 0 m m . T h e f l o w c e l l i s m a d e o f q u a r t z w i t h a t i t a n i u m h o u s i n g . - Page 33 Reference information Monochromator Detectors and electronics Flow cell Beam Block splitter filter Con- densor Flash lamp 100 Hz T h e l o n g p a t h l e n g t h a n d s m a l l v o l u m e o f t h e f l o w c e l l e n s u r e v e r y h i g h s e n s i t i v i t i e s a n d h i g h s i g n a l - t o - n o i s e r a t i o s .
- Page 34 Reference information Menus Check menu C h e c k i n g a u t o z e r o l e v e l T h e i n s t r u m e n t i n t e r n a l a b s o r b a n c e v a l u e f o r a u t o z e r o c a n b e c h e c k e d t o t e s t t h e c o n s i s t e n c y i n b u f f e r s .
- Page 35 6 T h e i n s t r u m e n t n a m e a n d s o f t w a r e v e r s i o n a r e d i s p l a y e d . T u r n t h e Monitor UV-900 V1.06...
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Page 36: S E T U P M E N
Reference information Setup menu S e t u p l a n g u a g e S e t s t h e l a n g u a g e u s e d i n t h e d i s p l a y . 1 S e l e c t m a i n m e n u , p r e s s S e t u p... -
Page 37: S E T T I N G A N D U S I N G T H E A L A R M T I M E
Reference information Setting and using the alarm timer Y o u c a n s e t t h e a l a r m f u n c t i o n t o e i t h e r a f i x e d a l a r m t i m e o r u s i n g a c o u n t - d o w n t i m e r . - Page 38 Reference information Menu overview Press OK to Set Wavelength λ1 Set Wavelength λ1 Set Wavelength λ1 λ1(280) 1.123 AU (215nm) (215nm) (215nm) λ2(254) 0.02345 AU Set Wavelength λ2 Set Wavelength λ2 Set Wavelength λ2 (215nm) (215nm) (254nm) λ3(215) Set Wavelength λ3 0.00000 AU Set Wavelength λ3 (215nm)
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Page 39: T E C H N I C A L S P E C I F I C A T I O N
Reference information Technical specifications O p e r a t i n g d a t a W a v e l e n g t h r a n g e 1 9 0 – 7 0 0 n m i n s t e p s o f 1 n m , 3 w a v e l e n g t h s s i m u l t a n e o u s l y B a n d w i d t h 4 n m... - Page 40 Reference information C h e m i c a l r e s i s t a n c e T h e w e t t e d p a r t s a r e r e s i s t a n t t o o r g a n i c s o l v e n t s a n d s a l t b u f f e r s c o m m o n l y u s e d i n c h r o m a t o g r a p h y o f b i o m o l e c u l e s , e x c e p t 1 0 0 % E t h y l a c e t a t e , 1 0 0 %...
- Page 41 Reference information T h i s d e v i c e m e e t s t h e r e q u i r e m e n t s o f t h e E M C s t a n d a r d s E M C D i r e c t i v e 8 9 / 3 3 6 / E E C t h r o u g h t h e f o l l o w i n g h a r m o n i z e d s t a n d a r d s : •...
- Page 42 Reference information Accessories and spare parts I t e m Q u a n t i t y p e r p a c k C o d e n o . M o n i t o r U V - 9 0 0 c o m p l e t e b u t w i t h o u t 1 8 - 1 1 0 8 - 3 5 f l o w c e l l s F l o w c e l l U V - 9 0 0 / 2 ( 2 m m )
- Page 43 Short instructions T h e f o l l o w i n g s h o r t i n s t r u c t i o n s a r e i n t e n d e d a s a g u i d e f o r u s e r s w h o a r e f u l l y f a m i l i a r w i t h t h e s a f e t y p r e c a u t i o n s a n d o p e r a t i n g i n s t r u c t i o n s d e s c r i b e d i n t h i s m a n u a l .
- Page 46 A l l g o o d s a n d s e r v i c e s a r e s o l d s u b j e c t t o t h e t e r m s a n d c o n d i t i o n s o f s a l e o f GE Healthcare Bio-Sciences AB t h e c o m p a n y w i t h i n G E H e a l t h c a r e w h i c h s u p p l i e s t h e m .