Sample Application - GE AKTApure User Manual

5 Operation

5.7 Sample application

5.7 Sample application

Introduction
This section describes the different sample application techniques that can be used with ÄKTA pure.
The table below shows the alternatives for sample application available in the Sample application
phase of a method.
Sample application
Inject sample directly
onto column
Inject sample from
loop
Note:
When using a pump for sample application, it is important to prime inlets and purge the pump before
using the pump to load the sample:
• Sample pump, see further instructions in Section 5.4.2 Sample pump, on page 168
• System pump A, see further instructions in Section 5.4.1 System pumps, on page 162
When loading sample using System pump A and an external air sensor, the sensor should be installed
according to Adapter for air sensor, on page 84.
Note:
180
Via
• Sample pump
• System pump A
• System pump A and Air sensor
• Syringe
• Sample pump
• Sample pump and Air sensor
• System pump A
• System pump A and Air sensor
In order to avoid sample carry-over when switching techniques for loading samples, wash the
valve with buffer between the loading of two different samples. For example, when switching
from loading sample in the loop to loading sample directly onto the column with the valve in
Direct inject position.
When sample is loaded at high flow rate and the external air sensor is placed before the pump
that is used for loading the sample, it is necessary to use longer tubing to ensure that no air
reaches the pump. Use tubing with the minimum lengths given below between the valve located
before the pump and the external air sensor. The length applies for maximum flow rate, but
shorter tubing can be used at lower flow rates.
• Sample pump S9: 40 cm.
• Sample pump S9H: 20 cm.
Compatible loops
• Not applicable
• Sample loop
• Superloop, 10 ml
• Superloop, 50 ml
• Superloop, 150 ml
ÄKTA pure User Manual 29119969 AB