Column Valve C Tests - GE AKTApure User Manual

6.5 Column valve C tests

Column valve C tests

The following Column valve C tests are available, for ÄKTA pure 25 and ÄKTA pure 150, respectively:
• Column valve V9-C test
• Column valve V9H-C test
Method description
The Column valve C test checks the functionality of Column valve C, labeled V9-C or V9H-C, and of the
integrated pressure sensors that measure pre-column and post-column pressure.
The method run takes approximately 1 minute.
Required configuration
A correctly installed Column valve C is required to run the test.
Required material
The following materials are required:
• Syringe, 25-30 ml
• Distilled water
• Ref 1 tubing, see Reference capillary, on page 426.
Prepare the test
Follow the instructions below to prepare the system before method start.
Step
1
2
3
4
5
ÄKTA pure User Manual 29119969 AB
Action
Connect the Ref 1 tubing between Column valve C ports 1A and 1B.
Make sure the Flow restrictor is inline.
Direct outlet tubing W to a waste container.
Place inlet tubing A1 into distilled water.
Prime the inlet A1 and purge the pump. See Section 5.4.1 System pumps, on page 162.
6 Performance tests
6.5 Column valve C tests
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