Sample Pump Tests - GE AKTApure User Manual

6 Performance tests

6.8 Sample pump tests

6.8 Sample pump tests

Sample pumps tests
The following Sample pump tests are available, for ÄKTA pure 25 and ÄKTA pure 150, respectively:
• Sample pump S9 test
• Sample pump S9H test
Method description
The Sample Pump test checks the functionality of the sample pump, labeled S9 or S9H.
The method run takes approximately 12 minutes.
Required configuration
The core components of ÄKTA pure and a correctly installed Sample pump are required to run the test.
Required material
The following materials are required:
• Distilled water
• 1% Acetone and 1.00 M NaCl in distilled water
• Syringe, 25-30 ml
• Ref 1 tubing, see Reference capillary, on page 426.
Prepare the test
Follow the instructions below to prepare the system before method start.
Step
1
2
3
4
5
242
Action
Connect a tubing between Injection valve port column and the UV inlet, preferably the
Ref 1 tubing included with the system at delivery.
Immerse waste tubing W, W1 and W2 in a waste container.
Immerse inlet tubing A1 in distilled water.
Immerse sample inlet tubing Buffer in 1% Acetone and 1.00 M NaCl in distilled water.
Prime inlet tubing A1 and sample inlet Buffer and purge the pumps. See Section 5.4.2
Sample pump, on page 168 and. No air shall be left in the pumps or tubings.
ÄKTA pure User Manual 29119969 AB