Calibrate The Pressure Monitors - GE AKTApure User Manual

7.7.2

Calibrate the pressure monitors

Maintenance interval
ÄKTA pure can have up to four pressure monitors: System pump pressure monitor, Sample pump
pressure monitor, Pre-column pressure monitor and Post-column pressure monitor. Check the pressure
monitors every week, or when the the ambient temperature has changed by more than ± 5°C. Calibrate
the monitor if the zero pressure reading is outside the range ± 0.02 MPa.
Check the monitors
Follow the instructions below to check the pressure monitors. The procedure is the same for each
monitor.
Step
1
2
3
4
Calibrate the monitors
Follow the instructions below to calibrate any of the pressure monitors.
Step
1
2
3
4
ÄKTA pure User Manual 29119969 AB
Action
Disconnect the relevant tubing from the pressure monitor to obtain zero-pressure, see
table Tubing and pressures, on page 306.
Click the Customize icon to open the Customize dialog. In the Customize dialog, under
the Run Data Groups tab, select the relevant pressure to display, see table Tubing and
pressures, on page 306. Click OK to close the Customize dialog.
In the Run Data pane in the System Control module, check what pressure is displayed.
If the zero pressure reading is outside the range ± 0.02 MPa, calibrate the pressure
monitor according to the instruction below.
Action
Disconnect the relevant tubing from the pressure monitor, see table Tubing and pressures,
on page 306.
In the Calibration dialog, select the pressure monitor to calibrate from the Monitor to
calibrate drop-down list.
Click the Reset pressure button.
Result: The atmospheric pressure is defined as zero. The date and time of the most recent
calibration, and the current pressure value are displayed.
Reconnect the tubing to the pressure monitor.
7 Maintenance
7.7 Calibration procedures
7.7.2 Calibrate the pressure monitors
305