Sample Application Using Direct Injection Onto The Column - GE AKTApure User Manual

5.7.1

Sample application using direct injection onto the column

Introduction
There are two ways to load sample directly onto a column:
• a fixed volume is loaded, or
• all the sample is loaded.
To inject all the sample, one of the following configurations is required:
• system pump, external air sensor, inlet valve A and the mixer valve, or
• sample pump and sample inlet valve.
Minimize sample loss
To minimize sample loss during direct injection of sample onto the column, sample remaining in the
flow path will be pushed onto the column with buffer from the inlet valve. This step is called Finalize
sample injection in the text instructions of the sample application phase of the method to be used.
Refer to Section 5.9 Create a method and perform a run, on page 211 for more information on methods
and phases.
When preparing to inject...
a fixed volume of sample
ÄKTA pure User Manual 29119969 AB
5.7.1 Sample application using direct injection onto the column
Then...
• manually prime the sample inlet tubing with sample, see Prime
sample inlets, on page 168.
• in the Method editor, make the following selections for the
Sample Application phase of the method to be run:
- select Inject sample directly onto column,
- select Inject fixed sample volume and set the volume to be
injected.
• make sure that the flow path from the sample inlet valve up to
the injection valve will be filled with an appropriate buffer:
- make sure that the buffer inlet tubing of the inlet valve is im-
mersed in buffer, and
- enable the function Wash sample flow path with buffer in
the Sample application phase.
5 Operation
5.7 Sample application
181