Perform Column Cip - GE AKTApure User Manual

7 Maintenance
7.6 Maintenance when required

7.6.3 Perform Column CIP

7.6.3

Perform Column CIP

Maintenance interval
Perform a Column cleaning in place (Column CIP) when required, for example between runs where
different samples are used.
Required material
The following materials are required:
• Appropriate cleaning solutions. Please refer to the instructions for use of the column.
Introduction
The Column CIP method is used to clean the column after purification runs, to remove non-specifically
bound proteins and to minimize the risk for carry-over between different purification runs.
Tip:
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WARNING
Hazardous biological agents during run. When using hazardous biological agents,
run System CIP and Column CIP to flush the entire system tubing with bacteriostatic
solution (e.g., NaOH) followed by a neutral buffer and finally distilled water, before
service and maintenance.
CAUTION
Hazardous substances. When using hazardous chemical and biological agents,
take all suitable protective measures, such as wearing protective glasses and gloves
resistant to the substances used. Follow local and/or national regulations for safe
operation, maintenance and decommissioning of the equipment.
If hazardous chemicals are used for system or column cleaning, wash the system or columns
with a neutral solution in the last phase or step.
ÄKTA pure User Manual 29119969 AB