Fraction Collector F9-C Test - GE AKTApure User Manual

6.6 Fraction collector F9-C test

Method description
The Fraction Collector Test checks the functionality of Fraction collector F9-C.
The method run takes approximately 6 minutes.
Required configuration
The core components of ÄKTA pure, an outlet valve and a correctly installed Fraction collector F9-C
are required to run the test.
Required material
The following materials are required:
• Distilled water
• Syringe, 25-30 ml
• Two deep well cassettes
• Two 96 deep well plates for collecting the fractions
Prepare the test
Follow the instructions below to prepare the system before method start.
Step
1
2
3
4
5
6
ÄKTA pure User Manual 29119969 AB
Action
Immerse waste tubing W in a waste container
Immerse inlet tubing A1 in distilled water.
Prime inlet tubing A1 and purge System pump A. See Section 5.4.1 System pumps, on
page 162. No air shall be left in the pump or tubing.
Place two 96 deep well plates in positions 1 and 6 in the fraction collector. Make sure
that no other cassettes are present in the fraction collector during the run.
In the System Control module, select System:Settings.
Result: The System Settings dialog opens.
In the System Settings dialog:
• Select Fraction collection:Fractionation settings.
• In the Fraction mode field, select Automatic.
• In the Fractionation order field, select Row-Automaticby-Row.
• Click OK.
6 Performance tests

6.6 Fraction collector F9-C test

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