Perform System Cip - GE AKTApure User Manual

7 Maintenance
7.6 Maintenance when required

7.6.2 Perform System CIP

7.6.2

Perform System CIP

Maintenance interval
Perform a System cleaning in place (System CIP) when required, for example between runs where dif-
ferent samples and buffers are used. This is important to prevent cross-contamination and bacterial
growth in the instrument.
Required material
The following materials are required:
• Appropriate cleaning solutions (e.g., NaOH, buffer solution or distilled water).
• Syringe, 25-30 ml
Introduction
The System CIP method is used to fill the instrument and the selected inlets and outlets with cleaning
solution.
Tip:
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WARNING
Hazardous biological agents during run. When using hazardous biological agents,
run System CIP and Column CIP to flush the entire system tubing with bacteriostatic
solution (e.g., NaOH) followed by a neutral buffer and finally distilled water, before
service and maintenance.
CAUTION
Hazardous substances. When using hazardous chemical and biological agents,
take all suitable protective measures, such as wearing protective glasses and gloves
resistant to the substances used. Follow local and/or national regulations for safe
operation, maintenance and decommissioning of the equipment.
CAUTION
Explosion hazard if flammable liquid leaks during cleaning of the flow path. When
cleaning the flow path of Fraction collector F9-C with a flammable liquid like ethanol,
carefully inspect the flow path, including the waste tubing, to make sure there will
be no leakage.
If hazardous chemicals are used for system or column cleaning, wash the system or columns
with a neutral solution in the last phase or step.
ÄKTA pure User Manual 29119969 AB