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3.4.1 Run Control Buttons
You clicked the Play button to start the run. During the run,
other run control buttons are active.
• Pause — The Pause button holds the %B at the current
value while the system continues to operate (sometimes
called an isocratic hold). Note that pausing the run
extends the run length. While in the Paused state, you
can resume the run by clicking the Play button, or stop
the run by clicking the Stop button. If you resume the
run, the system continues with the gradient curve at the
%B when the system was paused.
• Stop — This button suspends the entire run. Unlike the
Paused state, the pump, peak detection, and fraction
collection will not operate. While stopped, you can save
the run data by selecting the File>Save Run as PDF or
Save Run as Text from the menu, end the run by clicking
the Rewind button, or resume the run by clicking the
Play button.
• Next Tube — This button advances the fraction
collector to the next tube position. This allows you to
conveniently collect elute of interest in a new tube.
Note
When you click the Next Tube button, the system immediately
marks the tube advance on the chromatogram. However, the
system delays the movement of the drop former over the tubes.
This delay accounts for the volume held in the tubing between the
detector and the drop former, sometimes called the "delay
volume." It is important to remember that the chromatogram
displays activity in real time while the fraction collector activity lags
behind. The duration of this delay will vary with the flow rate.
• Rewind — This button is active when the system has
completed the run, or if the run was stopped by clicking
the Stop button. The Rewind button returns you to the
Main window with the current method.
If you have stopped the run before its programmed run
length has elapsed, clicking the Rewind button changes the
current method to the new, shorter run length. This modi-
fied method is ready for the next run, or can be saved for
future runs.
Section 3 Operation
3-7
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