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CombiFlash® R
Installation Guide
f
remain bound to the media. Once dried, pour the
media/sample mixture into an empty cartridge.
To prepare an empty solid sample cartridge:
1. Ensure the empty cartridge has a bottom frit.
Missing frits may cause equipment damage, UV detection
problems, or increased maintenance. Frits prevent solids from
entering the fluid path.
2. Prepare the media and sample:
a. Dissolve your sample in a minimal amount of a suitable,
volatile solvent.
b. Place the media into the solvent. If using silica gel, a
particle size of 40–60 µm (240–400 mesh) is
recommended. The amount of silica required is about
four to five times the mass of your sample.
c. Agitate the solvent for a moment to allow the sample to
adsorb to the silica.
d. Remove the solvent with a suitable method, such as
rotary evaporation.
3. Load the media and sample mixture into the cartridge. Tap
the cartridge on the benchtop to settle the mixture.
4. Place a frit on the top of the cartridge. Force the frit down
against the mixture using the plastic plunger.
5. Wipe any residual powder inside the neck of the cartridge.
Empty solid sample cartridges also allow a variety of
adsorbents, such as diatomaceous earth, boiling chips, cotton
balls, or paper wipes. Even with alternative adsorbents,
always use top and bottom frits to prevent fluid path
problems.
After you have prepared the pre-filled or empty cartridge,
place the solid sample cartridge on the system:
1. Attach the desired adjustable cartridge cap to the system.
2. Align the cap and cartridge and push them together.
3. Rotate the cartridge
cartridge.
3-2
CAUTION
/
1
turn so that the cap will hold the
4
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