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When using methanol as solvent B with silica column media, do
not pump more than 50% solvent B. Higher percentages of
methanol might break down the silica structure, possibly causing
obstructions in the flow path.
5.4.2 Quick Cleaning when Recommended
When the lamp energy is lower than normal, the system will
recommend flow cell cleaning (Figure 5-4) before starting a
separation run.
Figure 5-4 Flow cell cleaning message
When the system displays this message you can:
• Cancel Run (recommended) – Click the Cancel Run
button so you can perform a quick cleaning described in
the following steps.
• Continue Collect All – Click this button to ignore the
message. Because the peak detection operation might be
impaired, the system automatically collects all fluids to
avoid diverting compounds of interest to waste.
• Help – Click this button to display the flow cell cleaning
on-line help topic.
To perform a quick cleaning:
1. After clicking the Cancel Run button, select the
Tools>Manual Control menu option.
2. From the Manual Control window (Figure 5-5), note the
Raw Lamp Energy level at 254 nm. The Raw Lamp Energy
gauge has three ranges: red, yellow, and green.
CAUTION
Section 5 Maintenance
5-5
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