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• Cartridge w/ (with) pause – Select this option if
you intend place the sample into a solid sample load
cartridge, but have not yet prepared the sample.
When you click the run button, the system will
perform a column equilibration and then wait while
you prepare the cartridge. After you have placed the
cartridge on the system, click OK to continue with the
run.
• Cartridge w/o (without) pause – Select this option
if you have prepared the sample and placed it into a
solid sample load cartridge. The system will proceed
automatically until the end of the programmed run
length.
• Liquid Inject – Select this option if you have
prepared a liquid sample and plan to manually inject
it into the injection port after column equilibration.
• Preload on column – Select this option if you have
preloaded the sample on the column. The system will
skip column equilibration so that the sample will not
be flushed from the column before the run.
5. Review or update the Start Rack and Start Tube.
6. Review the Minimum Run Requirements. The system will
report the estimated solvent volumes, expected waste,
collection tube usage, etc. You can use this information to
verify that there will be enough solvent to complete the
run, the waste volume does not exceed the collection
container's capacity, and whether or not more tubes will be
required during the run.
Note
The system estimates the solvent volumes by monitoring the
solvent level in the container and the known usage rate. The
system continues to refine this estimate during operation.
Note
If the waste level sensing tube is inserted correctly into the waste
container, the system automatically will suspend operation before
an overflow condition might exist. To prevent the run from being
suspended before completing the run, ensure that the container
will hold the expected waste volume.
Section 3 Operation
3-5
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