Agilent Technologies 1260 Infinity II User Manual page 92

5
Preparing the Module
Setting up the Sampler
Table 9 Method parameter settings
92
Injection
Specify the injection volume (in µL) in the Injection
Volume field. The injection volume limits depend upon the
configuration settings of the autosampler. Note that this
setting is ignored when Pretreatment/Injector Program is
defined.
Needle Wash
Mark the Enable Needle Wash check box to specify an
injection with needle wash.
The following wash parameters can be specified:
• Mode: Select the wash location by clicking the down
arrow and selecting the needle wash mode from the
list. You can select from Flush Port (default location) or
Wash Vial.
• Time: This field is enabled only if you have selected
Flush Port. Specify a flush time in seconds between
1 and 100 in steps of 1.
• Location: enabled only if you have selected a Wash
Vial. Allows you to specify a wash location in the
format Vial X, where X is the vial number. Specify well
locations in the format P<Plate #>-<Row
Character><Column Number>, for example, P1-A1. The
values of <Row Character> and <Column Number>
depend on the wellplate type.
• Repeat: available only if you have selected Wash Vial.
You can specify up to 10 repeat washes.
Stoptime: enables you to set the time that the analysis
stops. Limits: 0.01 through 99999 min or As Pump/No
Limit.
Posttime: the Vialsampler remains in a post-run state
during the Posttime to delay the start of the next analysis.
A Posttime period can be used to allow the column to
equilibrate after changes in solvent composition (for
example after gradient elution). Limits: 0.01 through
99999 min or Off.
Agilent 1260 Infinity II Preparative Autosampler User Manual