Differential Interference Contrast Microscopy - Nikon Eclipse Ni-E Instruction Manual

Differential Interference Contrast Microscopy

1.3
Problem
The universal condenser's turret is at the
intermediate position.
The DIC slider is at the intermediate
position for an oil condenser.
The DIC slider for the objective is at the
Lack of visibility around
intermediate position.
periphery of field of view
Incomplete attachment of nosepiece.
The polarizer and an analyzer are at the
intermediate position.
The lambda plate is at the intermediate
position.
The polarizer is out of the optical path.
The analyzer is out of the optical path.
A correct DIC module on the condenser
has not been selected.
No contrast
The DIC slider for the objective is out of
the optical path.
Incorrect combination of the objective with
the DIC slider for the objective.
The immersion oil is not sufficient in the oil
condenser, and air bubbles are between
the slide and the condenser.
Chapter 2
Troubleshooting
Cause
43
Measure
Rotate it to the click stop position.
(→See Chapter 3 "9.3 Using the Universal
Condenser (Dry) NI-CUD".)
Push in the slider until it reaches the limit.
(→See Chapter 3 "9.4 Using the D-CUO DIC
Condenser (Oil)".)
Attach it correctly.
(→See Chapter 3 "17.2 Using Optical Elements".)
Attach it correctly.
(→See Chapter 1 "3 Assembly Method - 19 Attach
the nosepiece" in the "Assembly/Maintenance".)
Switch over correctly.
(→See Chapter 3 "17.2 Using Optical Elements".)
Make sure that it is attached to the limit.
(→See Chapter 3 "17.2 Using Optical Elements".)
Bring the polarizer into the optical path.
(→See Chapter 2 "5 Differential Interference
Contrast Microscopy Procedure - 14 Adjust the
orientation (vibration direction) of the polarizer and
analyzer" in the "Operation".)
Bring the analyzer into the optical path.
(→See Chapter 2 "5 Differential Interference
Contrast Microscopy Procedure - 14 Adjust the
orientation (vibration direction) of the polarizer and
analyzer" in the "Operation".)
Bring the DIC module suitable for the objective
to use into the optical path.
(→See Chapter 1 "3 Assembly Method - 18 Attach
the condenser - ■ Attach the optical module to the
universal condenser - ■ Attaching the DIC module
to DIC condenser" in the "Assembly/Maintenance".)
Bring it into the optical path.
(→See Chapter 2 "5 Differential Interference
Contrast Microscopy - 16 Attach the DIC slider (on
the objective) to the nosepiece" in the "Operation".)
Use a DIC slider suitable for the objective.
(→See Chapter 1 "3 Assembly Method - 18 Attach
the condenser - ■ Attaching the optical module to
the universal condenser, ■ Attaching the DIC
module to DIC condenser - ■ Attaching the DIC
module to DIC condenser" in the
"Assembly/Maintenance".)
Apply the immersion oil again.
(→See Chapter 3 "14 Oil/Water Immersion" in the
"Operation".)