Epi-Fluorescence Microscopy - Nikon Eclipse Ni-E Instruction Manual

Epi-fluorescence Microscopy

1.2
Problem
Lack of visibility around
periphery of field of view.
Illumination is uneven
The filter cube is misaligned.
across the field of view.
Field of view is not
visible.
The shutter is closed.
A fluorescent image is
not visible (when the
lamp is ON).
The selection of the filter cube is incorrect.
The ND filters of the epi-fluorescence
attachment are in the optical path.
The fluorescent image is
very dark (when the
A halogen light source is used for a dark
lamp is ON).
specimen.
A designated objective is not used at UV or
V excitation.
The room is bright.
The dia-illumination lamp is on.
The filter cube being used is not suitable
for the specimen.
The fluorescent image
quality is poor.
The objective or cover glass is dirty.
The immersion oil is fluorescent.
The contrast of the
fluorescent image is
poor.
The slide glass is fluorescent.
Stray light is entering from the condenser.
Chapter 2
Troubleshooting
Cause
42
Measure
Push the cube in to the limit.
(→See Chapter 1 "3 Assembly Method , 10 Attach
the epi-fluorescence cube turret and
epi-fluorescence attachment - ■ Attaching the filter
cube" in the "Assembly/Maintenance".)
Open the shutter.
(→See Chapter 3 "16.3 Protecting the Sample and
Preventing It from Decoloration (Using the Shutter)"
in the "Operation".)
Use a correct filter cube.
(→See Chapter 3 "16.2 Selecting Filters" in the
"Operation".)
Remove the ND filters from the optical path as
necessary.
(→See Chapter 3 Section 16.4 "Adjusting the
Brightness of the Fluorescent Image (Using ND
Filters and the Aperture Diaphragm)" in the
"Operation".)
Change the light source to a mercury lamp.
Use a designated objective.
Make it darker.
Turn off the dia-illumination lamp.
Use a filter cube suitable for the specimen.
(→See Chapter 3 "16.2 Selecting Filters" in the
"Operation".)
Clean it as appropriate.
(→See Chapter 3 "2.1 Cleaning Lenses" in the
"Assembly/Maintenance".)
Use the non-fluorescent immersion oil
designated by Nikon.
(→See Chapter 3 "16.9 Other Notes on
Epi-fluorescence Microscopy" in the "Operation".)
Use a non-fluorescent slide glass.
(→See Chapter 3 "16.9 Other Notes on
Epi-fluorescence Microscopy" in the "Operation".)
Lower the condenser, or remove the
condenser and attach a shielding tube.