Nikon Eclipse Ni-E Instruction Manual page 47

Problem
The field diaphragm image is not focused
on the specimen surface.
(Condenser adjustment is incorrect.)
No immersion oil is applied to the tip of an
oil-immersion objective.
Image quality is poor.
The designated immersion oil is not used.
Contrast is poor.
Resolution is poor.
The immersion oil contains air bubbles.
The immersion oil adheres to the tip of the
dry-type objective.
The ND filters are out of the optical path.
Field of view is too
bright.
The lamp voltage is too high.
The lamp voltage is too low.
The condenser aperture diaphragm is
stopped down too far.
Field of view is too dark.
The field diaphragm image is not focused
on the specimen surface.
The optical path is not switched to
binocular 100%.
The NCB11 filter is not used.
Image is yellowish or
very bluish.
The lamp voltage is too low or too high.
Visually observed image
color does not match White balance of the camera is not set
correctly.
color of image on
monitor.
Filter cube is in the optical path even
The entire field of view is
though epi-fluorescence observation is not
bluish or yellowish.
being performed.
Chapter 2
Troubleshooting
Cause
39
Measure
Make sure the condenser is focused and
centered.
(See Chapter 3 "7 Focusing and Centering the
Condenser" in the "Operation".)
Apply our designated non-fluorescent
immersion oil.
(→See Chapter 3 "14 Oil/Water Immersion" in the
"Operation".)
Remove the air bubbles.
(→See Chapter 3 "14 Oil/Water Immersion" in the
"Operation".)
Clean it as appropriate.
(→See Chapter 3 "14 Oil/Water Immersion" in the
"Operation".)
Place the ND filters in the optical path.
(→See Chapter 3 "3.2 Adjustment with ND Filters"
in the "Operation".)
Turn the brightness control knob to the
mark position and adjust the brightness with
the ND filters.
(→See Chapter 3 "3.2 Adjustment with ND Filters"
in the "Operation".)
Turn the brightness control knob to the
mark position and adjust the brightness with
the ND filters.
(→See Chapter 3 "3.2 Adjustment with ND Filters"
in the "Operation".)
This should normally be adjusted to 70 to 80%
of numerical aperture of the objective.
(→See Chapter 3 "8 Adjusting the Aperture
Diaphragm" in the "Operation".)
Make sure the condenser is focused and
centered.
(See Chapter 3 "7 Focusing and Centering the
Condenser" in the "Operation".)
Set to binocular 100%.
(→See Chapter 3 Section 12 "Switching the Optical
Path of the Tube", "Operation".)
Use the NCB11 filter.
(→See Chapter 3 "3.2 Adjustment with ND Filters"
in the "Operation".)
Turn the brightness control knob to the
mark position and adjust the brightness with
ND filters.
(→See Chapter 3 "3.2 Adjustment with ND Filters"
in the "Operation".)
Set the white balance in accordance with the
camera's instruction manual.
Remove the filter cube from the optical path.