Oblique Illumination; Polarization - Leica DM2000 Operating Manual

9. Contrast Methods
Special darkfield condensers are available for
the DM2000 and DM2500 (Fig. 61).
The application potential of the DF condensers
depends on the aperture of the objective in use.
For objectives with a built-in iris diaphragm, the
aperture can be adapted.
DF Condenser
D 0.80 - 0.95
D 1.20 - 1.44 OIL

9.1.4 Oblique Illumination

• First adjust transmitted light darkfield.
• To obtain a relief-like contrast:
Condenser UCA/P:
Rotate condenser disk slightly out of the DF
position.
Condensers CL/PH,
ACHR.0.9 (P):
Push DF slide in part way out of the DF posi-
tion.
Fig. 61 Darkfield condensers
1 Upper part (dry)
2 Lower part
3 Orientation pin
4 Upper part (oil immersion)
1
2
3
52
Max. Objective Aperture
0.75
1.10
CLP/PH und
4

9.1.5 Polarization

• Swing the lambda plate of the lambda plate
compensator out if necessary.
• Insert a specimen and rotate an appropriate
objective into place.
• Bring the image into focus and set the Köhler
illumination (→ p. 31).
• Insert the analyzer as far as the clickstop into
the left side of the stand (fig. 64). The engrav-
ing λ must be on the underside of the stand.
When using the Pol intermediate tube*:
Switch on the analyzer.
• Push the polarizer with the labelled side fac-
ing upward into the lower opening.
APL.
Fig. 62 Analyzer/polarizer
1 Analyzer slot
2 Polarizer mount
a b
1
2