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Summary of Contents for Leica DMIRE2
- Page 1 Cell Imaging Unit – UIC LEICA DMIRE2 MICROSCOPE MANUAL...
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Page 2: Table Of Contents
TABLE OF CONTENTS ANATOMY OF THE LEICA DMIRE2 INVERTED MICROSCOPE INITIALISATION PROCEDURE LEFT SIDE OF MICROSCOPE (DETAILED VIEW) DMIRE2 FRONT PANEL 1. LCD DISPLAY OF MICROSCOPE STATUS 2. LCD DISPLAY CONTROL BUTTONS 3. L.E.D. PANEL SHOWING ACTIVE FLUORESCENCE CUBE 4. FILTER CUBE SELECTION SWITCH 5. -
Page 3: Anatomy Of The Leica Dmire2 Inverted Microscope
ANATOMY OF THE LEICA DMIRE2 INVERTED MICROSCOPE 1. Binocular phototube 16. Filters 2. Eyepiece adapter tube 17. Aperture diaphragm adjustment 3. Eyepieces 18. Lamp housing mount 4. Tube mount 19. Lamp housing 5. Tube port for photo/TV connect 20. Stage plate 6. -
Page 4: Initialisation Procedure
INITIALISATION PROCEDURE 1. Ensure that both the DMIRE2 control box (CTRMIC) and the mercury arc lamp power supply have been switched on (shown below). After switching on the CTR6000 control box the microscope will take a few seconds to initialize 2. -
Page 5: Left Side Of Microscope (Detailed View)
LEFT SIDE OF MICROSCOPE (DETAILED VIEW) Halogen lamp intensity adjustment wheel Coaxial coarse & fine focus Beam splitter (not functional) Epifluorescence illumination diaphragm (controls brightness of illumination) Epifluorescence field diaphragm (controls area of illumination) Objective prism turret Filter cube turret... -
Page 6: Dmire2 Front Panel
DMIRE2 FRONT PANEL 1. LCD DISPLAY OF MICROSCOPE STATUS (ALPHA-NUMERIC). Displays the following information: • Position of objective lens relative to upper limit (e.g. -145µm) • Step size setting for focussing (S0-3) • Upper & lower limits for stage position (↑↓) •... -
Page 7: Lcd Display Control Buttons
2. LCD DISPLAY CONTROL BUTTONS Puts microscope in learn mode (DO NOT USE). If you press LEARN this button by accident, you will see the word “EXIT” flashing. Press the LEARN button again to exit the learn mode. Toggles between voltage readout and objective readout. CHANGE Set lower limit of travel for objective (DO NOT USE) Set upper limit of travel for objective (DO NOT USE) -
Page 8: Port Selection Switch
6. PORT SELECTION SWITCH (TWO WAY) Light directed to binocular eyepieces Light directed to scanner SIDE BOTTOM Disabled The active port will be illuminated (red) in the vertical LED panel. -
Page 9: Contrast Methods
CONTRAST METHODS BRIGHTFIELD Use the wheel on the front left side of the microscope stand near the base to turn on and adjust the halogen lamp brightness (shown below). The lamp voltage will be displayed automatically on the microscope control panel when the intensity is adjusted. -
Page 10: Magnification
MAGNIFICATION The following objective lenses are fitted on the DMIRE2: DIC prisms Pixel size Objective type N.A. Immersion Cover-glass (upper S1) prisms prisms (1xc- (upper (lower) mount) S23) (um/pixl) N PLAN 2,5x 0.07 With/without 2.58 0.30 With /without Not avail. (K11) 0.64... -
Page 11: Focussing
The upper key will focus up whereas the lower key will focus down. If an upper limit is set for the objective (See DMIRE2 Front Panel – LCD Display Control Buttons) then the objective will not move above that limit when using the focussing buttons. -
Page 12: Scanning Mode
SCANNING MODE 1. If you didn’t already does it start the Image-Pro Plus software available both as a Desktop shortcut and the Windows start menu. 2. On Image-Pro Plus press the small camera in the toolbar for acquisition. 3. On the Acquire dialog: Press “Preview”...
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