Uv Protection Screen; Dichroic Mirrors; Illumination - Leica FluoCombi III User Manual

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2.10 UV protection screen

Never work without UV protection
screen!
Fasten the holder with UV protection screen
(14.1) at the mount (14.2) using the clamping
screw (14.3).
Loosen the clamping screw (14.4).
Tilt the UV protection screen (14.1) so that the
operator cannot look directly onto the light spot.
Tighten the clamping screw (14.4).
2
3
4
5
1
Fig. 14 UV protection screen
1 UV protection screen with holder
2 Mount for UV protection screen
3 Clamping screw for fastening the
UV protection screen
4 Clamping screw for locking the
UV protection screen in the desired tilt
5 Slot with dichroic mirror
14

2.11 Dichroic mirrors

Use dichroic mirrors (2-color-coated) in the Fluo-
Combi III™ analogous to the filter sets used in the
filter changer of the stereomicroscope. The mount
of the dichroic mirror is magnetically fixed in the
mount to ensure a correct alignment.
Insert the mount with the dichroic mirror (Fig. 6) in
the slot for the filter slide (14.5) so that the guide
pin points down and the marking can be read on
the outside.
Dichroic mirror
GFP
GFP Plus
GFP Plant
UV
Blue
Green
Texas Red
CFP
YFP
DsRED

2.12 Illumination

according to the M2-160-0 operating instructions
so that you can achieve a homogenous light spot
and good fluorescence even in micro mode.
Leica FluoCombi III™ – Assembly
Please adjust the Hg lamp precisely
marking
GFP1
GFP2
GFP3
UV
B
G
TXR
CFP
YFP
DSR

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