Phase Contrast; Darkfield - Leica DM2000 Operating Manual

9.1.2 Phase Contrast

• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
Objectives that are suitable for phase contrast
are engraved with PH.
• Bring the image into focus using the focus dial
and set the brightness.
• For an optimal field diaphragm setting, check
the Köhler illumination (→ p. 31).
• Open the aperture diaphragm completely (po-
sition PH).
• Condensers UCL/UCLP and UCA/P:
Set the light ring corresponding to the objec-
tive on the condenser disk.
Example: Light ring 1 belongs to the objective
with the engraving PH 1.
Condensers CL/PH,
ACHR.0.9 (P):
Use the light ring slide.
Note:
Condensers UCL/UCLP and UCA/P: Light rings
must be centered (→ p. 33).
CLP/PH and APL.

9.1.3 Darkfield

• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Bring the image into focus using the focus dial
and set the brightness.
• Condenser UCA/P and UCL:
Click the condenser disk into the BF position.
Condensers CL/PH,
ACHR.0.9 (P):
Pull out the DF light ring slide as far as the
stop.
Check the Köhler illumination (→ p. 31).
• Open the aperture diaphragm completely (po-
sition PH).
• Condenser UCA/P and UCL:
Click the condenser disk into the DF position.
Condensers CL/PH,
ACHR.0.9 (P):
Insert the DF light ring slide as far as the stop.
Note:
Condensers UCL/UCLP and UCA/P: The DF light
ring must be centered (→ p. 33).
9. Contrast Methods
CLP/PH und APL.
CLP/PH und APL.
51