Leica DMI3000B Instructions Manual
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Leica DMI3000B Instructions Manual

Leica DMI3000B Instructions Manual

Inverted, manual microscope
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See also: Operating Manual
Leica DMI3000 B,
DMI4000 B, DMI6000 B
Instructions
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Summary of Contents for Leica DMI3000B

  • Page 1 Leica DMI3000 B, DMI4000 B, DMI6000 B Instructions...
  • Page 2 6.16 Optional Accessories......... 57 6.17 Connection to the Electronics Box ..58 6.18 Connection to the Computer ..... 59 Overview of the Leica DMI Series ..14 6.19 Connection to the Power Supply ..... 59 Unpacking the Microscope ..... 27 Start-up ............

  • Page 3: Table Of Contents

    Contents Operation ............. 81 Troubleshooting .......... 105 8.1 Switching on..........81 8.2 Contrast Methods ........83 10. Care of the Microscope ......109 8.2.1 Bright Field (TL) ........ 83 10.1 Dust Cover ........... 109 8.2.2 Phase Contrast (TL) ....... 85 10.2 Cleaning ............
  • Page 4 4. Overview of the Instruments Fig. 1 Left side, Leica DMI4000 B and DMI6000 B Eyepiece 11 Variable function buttons Eyepiece tube 12 Left side port Top port 13 Booster lens Intermediate pupil interface (Leica DMI6000 B fl uorescence microscopes only) LeicaScreen 14 Lamp mount (fl...
  • Page 5 4. Overview of the Instruments Fig. 2 Right side Leica DMI4000 B and DMI6000 B E-Focus buttons (Leica DMI6000 B only) Centering window (fl uorescence microscopes only) Focus wheel (motorized Leica DMI6000 B, Field diaphragm centering manual (fi ne) Leica DMI4000 B) (fl...
  • Page 6 4. Overview of the Instruments Fig. 3 Front view Leica DMI4000 B and Leica DMI6000 B LeicaScreen Front control panel Port switching Top port Manual transmitted light fi lters Bertrand lens centering...
  • Page 7 Travel in y 100% light to all eyepieces Focus Port selection Variable function buttons Magnifi cation selection (pre assigned at factory) 1x tube lens Fig. 4 General view Leica DMI4000 B and Leica DMI6000 B with SmartMove remote control module...
  • Page 8 7. Start-up 7. Start-up 7.1 Functional Principle (Leica DMI4000 B and Leica DMI6000 B) Thanks to its intelligent automation, the Leica DMI4000 B and DMI6000 B can be controlled using a variety of control elements. 1 . Intelligent Automation • Switching between contrast methods at the touch of a button. Light rings, DIC prisms, etc.
  • Page 9 7. Start-up Function Fixed Variable SmartMove Software (DMI4000 B and DMI6000 B) Function Function Function Rotary buttons buttons buttons knobs STP6000 Stand Stand 4000 6000 4000 6000 4000 6000 4000 6000 4000/6000 Select contrast method Change transmitted light/incident light axis Change to objective Teach-in parfocality Change operating mode (dry/imm)
  • Page 10 7. Start-up Possible Assignments for Variable Function Buttons on Stand and SmartMove For Leica DMI4000 B and Leica DMI6000 B: Function button Function Bright fi eld transmitted light Phase contrast transmitted light Interference contrast, transmitted light Dark fi eld transmitted light...
  • Page 11 Off switch. The signal lamp is lit when the in- light, and phase rings have been pre-centered strument is ready. (For the Leica DMI3000 B at the factory. It may be necessary to correct the please continue at 7.4. Function Buttons on the...
  • Page 12 7. Start-up 7.3 The LeicaDisplay Pictograms (Leica DMI 4000 B and DMI 6000 B) The screen displays the microscope’s current Contrast method settings. The content of the display depends on the features of the individual microscope. Objective/ For information on the abbreviations used, please Magnifi...
  • Page 13 fi ne focusing; the right-hand focus wheel for fi ne focusing only (a version of the Leica DMI3000 B with mir- rored focus controls is also available) • Light intensity: the transmitted light intensity...
  • Page 14 For information on the abbreviations used, please refer to the list → p. 63. Note: The Leica Application Suite (LAS) software is re- quired for changing the button assignments. Possible functions*: CHANGE CUBE CW CHANGE CUBE CCW INT FLUO ↑...
  • Page 15 7. Start-up Function Buttons on the Front Panel (Fig. 87) CUBE The CUBE 1 to CUBE 6 (87.6) buttons permit the direct selection of indi- 100% of the light goes to the eye piece vidual fi lter cubes, provided the se- (87.1).
  • Page 16 • Place a specimen on the stage. X and Y directions. • Focus on the specimen using the focus wheels. The image is focused using the knob 89.3 (Leica DMI6000 B only). • Adjust the light intensity. The height of the knobs can be adjusted to a •...

  • Page 17: Operation

    All of the contrast methods of the Leica DMI4000 Leica DMI3000 B: B and Leica DMI6000 B can be selected and con- • If necessary, adjust the TL bright fi eld position trolled via the variable function buttons and the at the fi...
  • Page 18 8. Operation • Use the TL/IL function button to switch to • Insert a transmitted light specimen. transmitted light (TL). • Rotate an appropriate objective into place. • Select the BF (bright fi eld) contrast method by pressing the variable button BF. •...

  • Page 19: Phase Contrast (Tl)

    8. Operation 8.2.2 Phase Contrast (TL) Leica DMI4000 B and Leica DMI6000 B: (integrated phase contrast, see 8.2.6) • Use the TL/IL function button to switch to transmitted light (TL). Leica DMI3000 B: • If necessary, adjust the TL bright fi eld position •...
  • Page 20 8. Operation 8.3 Fluorescence Leica DMI4000 B and Leica DMI6000 B: • Use the TL/IL function button to switch to fl uo- Leica DMI3000 B: rescence FLUO. The fi lter slider (5a.9, S.24) is used to operate the fl uorescence module.
  • Page 21 8. Operation Options • The intensity of the fl uorescence can be in- creased by using the booster lens (Fig. 112) on the left rear side of the stand (Fig. 113). If bright fl uorescence is required in the center of the fi...

  • Page 22: Combination Methods

    8. Operation 8.4 Combination Methods (Leica DMI4000 B and DMI6000 B) Note: Up to two combination methods are possible de- The manual analyzer (Fig. 110) must be used for pending on the features of the individual micro- the FLUO/DIC method as described in Chapter scope: 8.2.5, p.

  • Page 23: Focusing

    Open fi lter drawer The display will show The focus position can also be set using the Leica Application Suite (LAS). Set the focus position for the dry objective at the highest magnifi cation. The focus positions will...
  • Page 24 The Fine value varies to suit the current objective. Suit- Leica Application Suite (LAS) Software able values have been predefi ned. The assignments can be changed with the Leica Application Suite (LAS). Summary of pictograms When selecting Coarse, the positioning speed is the same for all objectives.

  • Page 25: Tubes

    8. Operation 8.6 Tubes 8.7 Port selection Leica DMI 3000 B and Leica DMI4000 B: Manual shifter rod activates and deactivates the Note: left-hand photo port. LEFT Close any unused tube openings, as otherwise 100% stray light can interfere with observation.

  • Page 26: Eyepieces

    - the percentage of light going to the port (100%, Note: 80%, 50%). The eyepiece’s aperture protector must be re- Optional Leica DMI 6000 B: moved or folded back, during microscopy while The bottom port selection function can be as- wearing eyeglasses. We recommend removing...

  • Page 27: Objectives

    8.9 Objectives Changing objectives Note: Leica DMI3000 B and Leica DMI4000 B: We recommend running a teach-in via the Leica Select objectives manually with the objective tur- Application Suite (LAS) software when using ret. eyepieces not included in the scope of deliv- The objective turret of the DMI4000 B is coded ery.
  • Page 28 8. Operation • For immersion objectives use the appropriate immersion medium. Caution! OIL: only use optical immersion oil according to DIN/ISO standards. Follow safety instructions for immersion oil! Cleaning → p. 109. Water immersion. IMM: Universal objective for water, glycerol, oil immersion.
  • Page 29 It is possible to use objectives for both operating When replacing objectives, you must perform modes. a teach-in for the new objectives in the Leica The mode can be assigned in the Leica Applica- Application Suite (LAS). A parfocality teach-in tion Suite (LAS).

  • Page 30: Stages And Object Displacement

    Leica DMI3000 B and Leica DMI4000 B: A variety of stage positions can be stored tempo- The motorized stages are controlled via a sepa- rarily in the Leica Application Suite (LAS). The XY rate control unit. position is stored, not the Z position.
  • Page 31 Magnifi cation factor: 1.6x. The selected factor is displayed on the LeicaS- creen and in the relevant fi eld of the Leica Ap- A slider switches between 1x and the magnifi ca- plication Suite (LAS), and is taken into account tion factor.
  • Page 32 If you continue turning to the left at 100% or to the right after 10%, a 0% position (shutter posi- tion) is set. Leica DMI4000 B and Leica DMI6000 B: • Adjust the intensity with the function buttons (118.4). The INT function buttons are always assigned to the currently active transmitted light (TL) or incident light (IL) axis.
  • Page 33 SmartMove suitable for observations with CCD cameras. Leica Application Suite (LAS/LAS AF) Leica DMI4000 B and Leica DMI6000 B: Software Both diaphragms have been set to suitable val- ues for the current objective and contrast meth- od at the factory.
  • Page 34 9. Trouble Shooting 9. Trouble Shooting Problem Cause/Remedy Stand The microscope does not respond. Ensure that the AC outlet has power. Ensure that the electronics box is connected to an AC outlet. Check the cable connections. Inform Service and have the supply unit fuse checked.
  • Page 35 9. Trouble Shooting Problem Cause/Remedy Bright fi eld The specimen can not be brought into focus. Use the correct immersion medium. Place the specimen on the stage with the cov- erslip facing down. Make sure that the cover glass thickness is correct and that it suits the indication on the objective.
  • Page 36 9. Trouble Shooting Problem Cause/Remedy Phase contrast No phase contrast is possible. The specimen is too thick. The cover glass is not placed evenly. Check the centering of the light rings (→ p. 74). Polarization No polarization contrast is possible. Bring the polarizer and analyzer into cross position until they reach maximum darkness (without specimen).
  • Page 37 9. Trouble Shooting Problem Cause/Remedy Fluorescence The image is completely dark (no fl uorescence). Open the shutter (→ p. 68). Select the incident light axis (IL) (→ p. 66). Check your specimen, e.g. its antibody binding. Insert a new lamp (→ p. 45, p.49ff). The fl...
  • Page 38 13. Abbreviations and Pictograms 13. Abbreviations and Pictograms Contrasting method → Magnifi cation Illumination Ports/Eyepiece Focus Lower focus stop not set Lower focus stop set Focus position not set Focus position set Shutter open Shutter closed Transmitted light fi lter Field diaphragm, rectangular Field diaphragm, round Aperture diaphragm...
  • Page 39 13. Abbreviations and Pictograms Aperture diaphragm Bright fi eld COMBI Combination method CUBE Fluo cube Dark fi eld incident/transmitted light Differential Interference Contrast Field diaphragm FLUO Fluorescence axis (incident light) Interference contrast, incident light Interference contrast, transmitted light Incident light Intensity Integrated modulation contrast Integrated phase contrast...
  • Page 40 14. Index 14. Index Abbreviations 113 Contrasting method 14, 65, 83 Fixed stage 35 Active ports 65 Controls 18, 60 Fixed stage (normal) 33 Correction for vision problems 97 Fluorescence 91, 108 AFC Adaptive Focus Control 17, 62, 95 Corrective mount 100 Fluorescence fi...
  • Page 41 Transmitted light Lamp power cable 58 Port selection 96 interference contrast 107 Lamp replacement 44 Port switching 22, 26 Leica CTR6000 electronics box 10, 18 Power input 10 Transmitted light lamp housing 24 Transmitted-light 69 LeicaScreen Power supply 59 Transmitted-light fi lter 22, 26...

This manual is also suitable for:

Dmi4000bDmi6000b

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