Thermo Scientific NanoDrop Lite Plus User Manual page 35

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NanoDrop Lite Plus User Guide
Frequently Asked Questions
A: Many protein reagents and buffers contain surfactants that may interfere with the
hydrophobic nature of the pedestals, causing samples to spread. Use the NanoDrop
PR-1 reconditioning compound as a rapid means of reconditioning the pedestals
when the surface properties have been compromised and liquid columns break
during measurement. PR-1 kits are available through Thermo Fisher Scientific or
your local distributor.
Q: Where is the spectrum from my measurement?
A: The NanoDrop Lite Plus does not collect spectral data. It measures absorbance
at four wavelengths: 230 nm, 260 nm, 280 nm, and 340 nm for the reference
wavelength.
Q. How do I clean the pedestals?
A: See "Cleaning" on page 29. Do not use detergents or isopropanol as cleaning
agents as their use may result in the pedestals becoming unconditioned. When the
pedestal becomes unconditioned sample droplets will not properly bead on the
bottom pedestal.
Q: How often do I need to run Performance Verification?
A: We recommend confirming that the instrument is working within specifications
about every 6 months. The Thermo Scientific Performance Verification (PV-1) is
required to run the Performance Verification check procedure. PV-1 is available from
Thermo Fisher Scientific or one of its authorized distributors.
Q: Does the NanoDrop Lite Plus require a computer to operate?
A: No, the NanoDrop Lite Plus instrument is a standalone unit with local control.
Data can be saved to a memory device and transferred to a computer.
Q: How long before I need to replace the light source in the NanoDrop Lite Plus?
A: The xenon flash lamp is expected to last for the lifetime of the instrument.
Q: Is the xenon flash lamp continuously on, or on only when performing a
measurement?
A: The xenon flash lamp is only on during measurements.
Q: Can I connect my computer to the NanoDrop Lite Plus?
A: No. The NanoDrop Lite Plus cannot be connected to a PC. The NanoDrop Lite
Plus is a local control instrument running stand-alone software.
Q: Are there solvents that will damage the pedestal measurement surfaces?
Thermo Scientific NanoDrop Lite Plus User Guide 35