Cleaning - Thermo Scientific NanoDrop Lite Plus User Manual

Troubleshooting

Cleaning

Thermo Scientific
2. Select Performance Verification.
3. Select View Previous.
4. To Export to USB, select Export.
• If the instrument does not pass the calibration check using 1 μL aliquots of PV-1,
immediately repeat the procedure again using 2 μL aliquots of PV-1.
• If the procedure fails with 1 μL volumes but passes with 2 μL volumes, it is an
indication that the pathlengths are within specifications but the pedestals may
not be properly conditioned. Clean the pedestals with PR-1 and repeat the
calibration check.
• If the instrument still does not pass the performance verification using 2 μL
volumes, recalibration is required. Contact technical support. Outside of the US
and Canada, please contact your local NanoDrop distributor.
The primary maintenance for the NanoDrop Lite Plus is to keep the pedestal
surfaces clean.
1. Pipette 3 μL of deionized water (dH
2. Do not use a squirt bottle to apply dH
instrument.
3. Lower the arm to form a liquid column; let it sit for approximately 2-3 minutes.
Wipe away the water from both the upper and lower pedestals with a dry, lint-free
lab wipe.
Tip Between measurements: Wipe the sample from both the upper and lower
pedestals with a clean, dry, lint-free lab wipe, to prevent sample carryover and
avoid residue buildup.
Tip Between users: A final cleaning of both pedestals with dH
recommended after the last sample measurement is collected.
Additional cleaning: When a more rigorous cleaning protocol is required (i.e. dried
proteins) substitute 0.5M HCl for the dH
3-5 μL of dH O.
2
1
NanoDrop Lite Plus User Guide
O) onto the bottom pedestal.
2
O or any other liquid to the surface of the
2
O in the procedure above. Follow with
2
NanoDrop Lite Plus User Guide
Maintenance
O is
2
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