Thermo Scientific NanoDrop Lite Plus User Manual page 33

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NanoDrop Lite Plus User Guide
Frequently Asked Questions
Q: What sort of reproducibility and dynamic range for nucleic acids should I expect
with the NanoDrop Lite Plus?
A: The dynamic range depends on the assay type. Refer to Table 3 for more details.
Q: What biomolecules can be analyzed with the NanoDrop Lite Plus?
A: The NanoDrop Lite Plus is designed for determining concentrations of purified
dsDNA, RNA, ssDNA, and proteins, that absorb at 260 nm or 280 nm.
Q: Do nucleic acids require purification prior to measurement?
A: Yes. Absorbance measurements are not specific for a particular nucleic acid. Any
biomolecule that absorbs at 260 nm (DNA, RNA or free nucleotides) will contribute to
the total absorbance of the sample.
Q: What happens to sample data if it does not get transferred to a USB device at the
time the measurement is made?
A: Data from each measurement is automatically saved in the instrument memory
and can be transferred to a memory device at a later time. Samples are identified by
the exact time the measurement was made. Data from up to 1000 measurements is
stored in the NanoDrop Lite Plus and can be transferred to a PC using the USB
device provided with the instrument or another compatible USB device. Once 1000
measurements have been stored in memory, measurement #1001 will be deleted
and will be replaced by #1.
Q: Can I quantify proteins using the NanoDrop Lite Plus?
A: Yes. The Protein A280 application can be used for purified proteins. The
NanoDrop Lite Plus does not support colorimetric assays.
Q. How do I view previous measurements stored in the instrument?
A. Use Sample History to view or print previous measurements. See "Printing
Labels" on page 25.
Q: What is the dynamic (concentration) range and reproducibility for proteins on the
NanoDrop Lite Plus?
A: The dynamic range depends on the assay type selected (1 Abs= 1 mg/mL, IgG,
BSA, or Other Protein). See Table 4 for more details.
Q: I am using a colorimetric method (e.g., Bradford, BCA, etc.) to determine the
protein concentration of my cell extracts. Can I measure the samples using the A280
method on the NanoDrop Lite Plus?
Thermo Scientific NanoDrop Lite Plus User Guide 33