Thermo Scientific NanoDrop Lite Plus User Manual
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Thermo Scientific NanoDrop Lite Plus User Manual

Thermo Scientific NanoDrop Lite Plus User Manual

Micro-uv spectrophotometer
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NanoDrop Micro-UV Spectrophotometer
NanoDrop Lite Plus
User Guide
S120 NanoDrop Lite Plus UG
Revision Date
March 2022

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Summary of Contents for Thermo Scientific NanoDrop Lite Plus

  • Page 1 NanoDrop Micro-UV Spectrophotometer NanoDrop Lite Plus User Guide S120 NanoDrop Lite Plus UG Revision Date March 2022...
  • Page 2 © 2022 Thermo Fisher Scientific Inc. All rights reserved. For U.S. Technical Support, please contact: For International Support, please contact: http://www.thermofisher.com/ Thermo Fisher Scientific NanoDropSupport 3411 Silverside Road Tatnall Building, Suite 100 Contact your local distributor. For contact Wilmington, DE 19810 U.S.A. information go to: Telephone: 302 479 7707 http://www.thermofisher.com/...

  • Page 3: Table Of Contents

    Contents Chapter 1 NanoDrop Lite Plus User Guide ......5 General Safety..........6 NanoDrop Lite Plus Basic Operation .
  • Page 4 Warranty ..........36 NanoDrop Lite Plus User Guide...

  • Page 5: Chapter 1 Nanodrop Lite Plus User Guide

    The NanoDrop Lite Plus comes with preloaded software and is designed to be used without a computer. The NanoDrop Lite Plus can also be connected to an accessory printer for printing measurement information on labels.

  • Page 6: General Safety

    Removal of the cover may also void the warranty. Note The NanoDrop Lite Plus is supplied with a 12 V power supply. Use only the power supply provided with the instrument. The unit also comes with a grounded power cord.
  • Page 7 NanoDrop Lite Plus User Guide NanoDrop Lite Plus Basic Operation USB for data transfer USB* DC power On/Off switch Printer dock connector * For factory calibration only Thermo Scientific NanoDrop Lite Plus User Guide...

  • Page 8: Menu Selection

    NanoDrop Lite Plus User Guide NanoDrop Lite Plus Basic Operation Menu Selection Connected hardware Applications Back Home History Settings Maintenance NanoDrop Lite Plus Home screen Table 1.Navigation menu Menu option Function Back Return to the previous screen Home Return to the Home screen...

  • Page 9: Measurement Basics

    Settings screen Maintenance screen Measurement Basics The NanoDrop Lite Plus spectrophotometer uses surface tension to hold a small volume of sample between two pedestals. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions.

  • Page 10: Nucleic Acid Measurements

    Nucleic Acid Measurements Nucleic acid samples can be easily checked for concentration and purity using the NanoDrop Lite Plus spectrophotometer. To measure nucleic acid samples (dsDNA, ssDNA and RNA) from the Home screen, select Nucleic Acids and select the appropriate Nucleic Acid application.

  • Page 11: Nucleic Acid Measurement Screen

    Sample measurement screen Nucleic Acid Calculations The NanoDrop Lite Plus should be used to measure the concentration of purified nucleic acid samples. Direct nucleic acid concentration measurements assume a purified sample as all absorbance at 260 nm is included in the calculation of the nucleic acid concentration.
  • Page 12 NanoDrop Lite Plus User Guide Nucleic Acid Measurements = (A *CF)/b where, c = the nucleic acid concentration in ng/μL A = the sample absorbance CF = the conversion factor in ng-cm/μL b = the pathlength in cm The generally accepted conversion factors for nucleic acids are: •...

  • Page 13: Protein Measurements

    NanoDrop Lite Plus User Guide Protein Measurements Protein Measurements Purified protein samples can be quantified on the NanoDrop Lite Plus spectrophotometer. To measure the concentration of a protein sample, select the Protein application from the Home screen and then select the appropriate assay type (1 Abs= 1 mg/mL, IgG, Lysozyme, BSA, or Other Protein).

  • Page 14: Protein Measurement Screen

    (mg/mL) using the selected extinction coefficient. The Protein A280 application does not require generation of a standard curve. The NanoDrop Lite Plus should be used to measure the concentration of purified protein samples. Direct protein concentration measurements assume a highly purified sample as all absorbance at 280 nm is included in the calculation of the protein concentration.
  • Page 15 NanoDrop Lite Plus User Guide Protein Measurements Protein concentration is calculated using the Beer-Lambert equation: = A/e*b where, c = the protein concentration A = the sample absorbance e = the protein specific mass extinction coefficient b = the pathlength in cm...

  • Page 16: Transferring Data From Instrument To Computer

    The NanoDrop Lite Plus automatically stores the last 1000 measurements in the internal memory. A USB memory device can be used to transfer these measurements from the NanoDrop Lite Plus to a computer for archival or further analysis. The internal software of the NanoDrop Lite Plus does not support connecting the NanoDrop Lite Plus to an external hard drive.

  • Page 17: Helpful Information For Measuring Samples

    NanoDrop Lite Plus User Guide Helpful Information for Measuring Samples Helpful Information for Measuring Samples Sample Volume Requirements Sample volume is important because it is essential that a proper liquid column is formed and that the path between the upper and lower pedestals is bridged with liquid sample which is held in place by surface tension.

  • Page 18: Sample Homogeneity

    • Heat high molecular weight DNA samples to 63 °C and gently vortex before measurement. Due to the small volumes required by the NanoDrop Lite Plus, it is important to ensure that the sample being measured is homogeneous. Field experience has shown that samples containing large molecules such as genomic or lambda DNA are particularly susceptible to heterogeneity.

  • Page 19: A260/A280 Ratio

    0.2-0.3. When comparing the NanoDrop Lite Plus to other spectrophotometers, it is important to ensure that the pH of an undiluted sample measured on the NanoDrop Lite Plus is at the same pH as the diluted sample measured on the second spectrophotometer.

  • Page 20: Run A Blanking Cycle

    NanoDrop Lite Plus User Guide Helpful Information for Measuring Samples Run a Blanking Cycle Run a blanking cycle to verify the following: • instrument is operating normally (with flat baseline) • pedestals are clean (i.e., no dried-down sample material on pedestals) •...

  • Page 21: Effect Of Evaporation And Solvent Usage

    DMSO can be used successfully. Using NanoDrop Lite Plus With Optional Printer To connect NanoDrop Lite Plus to printer 1. Ensure power supply is disconnected from the NanoDrop Lite Plus. Thermo Scientific NanoDrop Lite Plus User Guide...
  • Page 22 NanoDrop Lite Plus User Guide Using NanoDrop Lite Plus With Optional Printer 2. Remove the back cover from the NanoDrop Lite Plus. 3. Firmly push the printer onto the instrument. The printer will snap into place when properly docked. Connecting the NanoDrop Lite Plus to the printer To load paper into printer 4.
  • Page 23 The printer assembly will not work until it is properly docked to the NanoDrop Lite Plus. 5. Plug in the power supply and allow NanoDrop Lite Plus to initialize. 6. Open the printer cover by pushing the two release buttons at the rear while simultaneously swinging the panel back.
  • Page 24 NanoDrop Lite Plus User Guide Using NanoDrop Lite Plus With Optional Printer 9. Insert the paper roll (with the spool) into the paper roll holder. 10. Tap Feed from the Printer Settings to advance the paper. Tip Helpful tips for installing the paper •...

  • Page 25: Troubleshooting Paper Feed

    NanoDrop Lite Plus User Guide Using NanoDrop Lite Plus With Optional Printer Troubleshooting Paper Feed If the paper does not feed properly (no paper comes out from the top of the printer), remove the paper from the printer and repeat steps through 9.

  • Page 26: Printer Output

    Maintenance Calibration All NanoDrop Lite Plus instruments are calibrated at the time of manufacture and include a Factory Test Report. Should recalibration ever become necessary, this procedure must be performed by Thermo Fisher Scientific or the authorized service providers at our dealers.

  • Page 27: Performance Verification

    Maintenance Performance Verification All NanoDrop Lite Plus instruments are calibrated at the time of manufacture and include a Factory Test Report. It is recommended that a performance verification be performed every six months to verify the instrument is performing within specifications.
  • Page 28 NanoDrop Lite Plus User Guide Maintenance 12. Repeat Steps until 10 measurements have been collected. Tip To ensure accurate results, be sure to wipe the top and bottom of the pedestal with a dry, lint-free laboratory wipe between measurements. Use a fresh aliquot of PV-1 and a fresh pipette tip for each measurement.

  • Page 29: Cleaning

    Contact technical support. Outside of the US and Canada, please contact your local NanoDrop distributor. Cleaning The primary maintenance for the NanoDrop Lite Plus is to keep the pedestal surfaces clean. 1. Pipette 3 μL of deionized water (dH O) onto the bottom pedestal.

  • Page 30: Reconditioning

    NanoDrop Lite Plus User Guide Maintenance Reconditioning The pedestal surface may become unconditioned over time, especially when measuring proteins or samples that contain surfactants or detergents. If the surface properties have been compromised, samples may not “bead up” on the pedestal or the liquid column may break during measurement.

  • Page 31: Solvent Compatibility

    This will “re-condition” the surfaces allowing the liquid sample column to form. Solvent Compatibility The NanoDrop Lite Plus spectrophotometer pedestals are compatible with most solvents typically used in life science laboratories. These include: • acetone •...
  • Page 32 HCl. Frequently Asked Questions Q: What are the sample size requirements for the NanoDrop Lite Plus? A: As little as 1 μL is usually sufficient for most applications. Generally all additives including protein, DNA, RNA, buffer salts, and detergent-like molecules reduce the surface tension by interfering with the hydrogen bonding between water molecules.
  • Page 33 Data from up to 1000 measurements is stored in the NanoDrop Lite Plus and can be transferred to a PC using the USB device provided with the instrument or another compatible USB device. Once 1000 measurements have been stored in memory, measurement #1001 will be deleted and will be replaced by #1.
  • Page 34 NanoDrop Lite Plus User Guide Frequently Asked Questions A: No. The Protein A280 application in the NanoDrop Lite Plus is only applicable to purified proteins. Colorimetric assays such as BCA, Pierce 660 nm, Bradford, and Lowry are generally used for complex protein samples such as cell lysates and require a standard curve.
  • Page 35 Data can be saved to a memory device and transferred to a computer. Q: How long before I need to replace the light source in the NanoDrop Lite Plus? A: The xenon flash lamp is expected to last for the lifetime of the instrument.
  • Page 36 NanoDrop Lite Plus User Guide Warranty A: The NanoDrop Lite Plus measurement pedestals are compatible with most solvents typically used in a life science laboratory including dilute acids as long as they are immediately wiped from the pedestals when the measurement is complete.

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