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Related Manuals for Thermo Scientific NS-FANT12-CH
Summary of Contents for Thermo Scientific NS-FANT12-CH
- Page 1 Thermo Fisher Scientific RC B Instruction Manual 50125612-3 dicembre 2010...
- Page 2 IMPORTANT! Please reference this page for the most up-to-date information on the following* • web site addresses • contact information • copyright and trademark information *All subsequent pages in this manual may have incorrect web site addresses and contact information. ©2013 Thermo Fisher Scientific Inc.
- Page 4 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96 It is marked by the following symbol: Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device.
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Page 5: Table Of Contents
Table of Contents Preface ............................iii Scope of Supply ........................iii Intended Use ..........................iv Accident Prevention ........................ iv Precautions ..........................iv Chapter 1 Introduction and Description ....................1-1 Characteristics of the RC BIOS ................1-2 Technical Data ......................1-3 Directives, Standards and Guidelines ..................1-4 Functions and Features ......................1-4 Mains Supply ......................1-5 Rotor Selection ......................1-5... - Page 6 Contents Programmed Operation ................... 4-6 Using Advanced Features (Options) ................. 4-8 Temperature Control ..................... 4-13 Rotor Temperature Equilibration ................4-14 Chapter 5 Customer Control Inspection ....................5-1 Speed Controls ......................5-2 Timer Controls ......................5-2 Temperature Controls ..................... 5-3 Chapter 6 Maintenance and Care ......................
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Page 7: Preface
Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Thermo Fisher Scientific. It is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty. -
Page 8: Intended Use
Intended Use Intended Use • This centrifuge is a laboratory product designed to separate components by generation of Relative Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives. •... - Page 9 Precautions • Do not use rotors which show any signs of corrosion and/or cracks. • Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components. • Use only with rotors which have been properly installed. Follow the instructions in section “Rotor Installation”...
- Page 10 Precautions • If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate disinfection measures have to be taken (see “Disinfection” page 6-4). • Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes.
- Page 11 Introduction and Description Contents “Characteristics of the RC BIOS” page 1-2 • • “Technical Data” page 1-3 “Directives, Standards and Guidelines” page 1-4 • • “Functions and Features” page 1-4 “Mains Supply” page 1-5 • • “Rotor Selection” page 1-5 Thermo Scientific RC B...
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Page 12: Chapter 1 Introduction And Description
Introduction and Description Characteristics of the RC BIOS Characteristics of the RC BIOS The maintenance-free induction motor ensures quiet and low-vibration operation even at high speeds, and guarantees a very long lifetime. The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time, temperature, and running profile (acceleration and braking curves). -
Page 13: Technical Data
Introduction and Description Technical Data Technical Data The technical data of the RC BIOS is listed in the following table. Table 1-1. Technical Data RC B Feature Value Environmental conditions -Use in interior spaces -Altitudes of up to 2,000 m above sea level -max. -
Page 14: Directives, Standards And Guidelines
Introduction and Description Directives, Standards and Guidelines Directives, Standards and Guidelines Table 1-2. Directives, standards and guidelines Tension / Produced and inspected according to the following Frequency standards and guidelines 220V-230V 50Hz, 2006/95/EC Low Voltage Directive: EN 61010-1, 2 Edition 400V 50Hz 2006/42/EC Machine Directive: EN 61010-2-020, 2... -
Page 15: Mains Supply
Introduction and Description Mains Supply Mains Supply The following table contains an overview of the electrical connection data for the RC B . This data is to be taken into consideration when selecting the mains connection socket. Table 1-4. Electrical connection data of the RC BIOS Rated cur- Power con- Equipment... - Page 16 Before use Contents “Before Setting up” page 2-2 • “Location” page 2-2 • “Installation” page 2-2 • “Aligning the Centrifuge” page 2-5 • “Transporting the Centrifuge” page 2-5 • “Mains Connection” page 2-5 • • “Other connections” page 2-6 “Storage” page 2-6 •...
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Page 17: Chapter 2 Before Use
Before use Before Setting up Before Setting up 1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately if any damage is discovered. 2. Remove the packaging. 3. Check the order for completeness (see “Scope of Supply”... - Page 18 Before use Installation 4. Place the piece of wood under the rails. Rails 5. Place the rubber feet under the studs with the safety brackets and screw them entirely up. 6. Remove the four screws that fix the centrifuge t the pallet. The heads are upsiede down in the pallet.
- Page 19 Before use Installation 10. Check that the stearing casters point to the back. 11. Use several people and other means in order to roll the centrifuge off the pallet. WARNING Due to the centrifuges weight it must be controlled by several people and other means when rolling of the pallet.
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Page 20: Aligning The Centrifuge
Before use Aligning the Centrifuge Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location. The supporting structure must be suitable for horizontal setup of the centrifuge. Transporting the Centrifuge •... -
Page 21: Other Connections
Before use Other connections Other connections 9 Pol D-Sub Connector Barcode Reader D-Sub Computer Interface Connector Figure 2-1. Connetors to external devises Storage • Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. •... -
Page 22: Control Panel
Control Panel Contents “KEYS” page 3-2 • • “CONTROLL PANEL” page 3-2 “RUN DISPLAY” page 3-3 • • “PRIMARY FUNCTION KEYS” page 3-4 “SET DISPLAY” page 3-7 • • “KEYPAD LOCK” page 3-8 Thermo Scientific RC B... -
Page 23: Controll Panel
Control Panel CONTROLL PANEL CONTROLL PANEL The controll panel consists of the RUN DISPLAY, the SET DISPLAY, the MENU key with the OPTION INDICATORS, the PRIMARY FUNCTION KEYS, a NUMMERIC KEY PAD, and a KEYPAD LOCK. RUN DISPLAY OPTION INDICATORS KEYPAD LOCK MENU KEY NUMERIC KEYPAD... -
Page 24: Run Display
Control Panel RUN DISPLAY Press RECALL to view and select programmed parameters. Press MENU to enter advanced features. RUN DISPLAY The RUN DISPLAY shows the actual values during a run. SPEED field The SPEED (RPM/RCF) field indicates either: • the current measured rotor speed (in rpm) or, •... -
Page 25: Primary Function Keys
Control Panel PRIMARY FUNCTION KEYS PRIMARY FUNCTION KEYS The Primary Function keys are the keys below the SET DISPLAY. ROTOR allows selection of rotors to be used. SPEED allows changing desired speed (rpm) or RCF (g-force). TEMP allows changing desired calculated sample temperature. RECALL allows viewing and selecting programmed parameters. - Page 26 Control Panel PRIMARY FUNCTION KEYS Note When changing mode, rpm/RCF values may appear a digit or two off from the original set value. The centrifuge translates set RCF values to rpm whole numbers for speed control purposes, then calculates that rpm whole number to the closest RCF value. There is no cause for concern. Speed control accuracy is not compromised, and the slight value difference will not affect your run The run duration (from start to termination, when deceleration begins) setting or control method is changed by pressing one of these three PRIMARY FUNCTION KEYS:...
- Page 27 Control Panel PRIMARY FUNCTION KEYS • When START is pressed, the realized ACE value begins to accumulate in the RUN DISPLAY. • The run will terminate and deceleration will begin when the specified ACE value (expressed as ω value up to 9.99 x 10 [displayed as 9.99e30]) is reached.
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Page 28: Set Display
Control Panel SET DISPLAY TEMP Press the TEMP key to change the desired calculated sample temperature ( °C) that is controlled by the centrifuge during a run. The centrifuge automatically considers the rotor selected, rotor speed, run time, set temperature, and measured temperature to calculate and maintain sample temperature during the run. -
Page 29: Keypad Lock
Control Panel KEYPAD LOCK KEYPAD LOCK • The KEYPAD LOCK can be used to limit control panel function. • It uses a 3-position keyswitch with a key that is removable in any of the 3 positions. Functionality is as follows: •... -
Page 30: Operation
Operation Contents “Switch on Centrifuge” page 4-2 • • “Lid Opening” page 4-2 “Close Lid” page 4-2 • • “Rotor Installation” page 4-3 “Normal Operation” page 4-3 • • “Centrifugation” page 4-5 “Programmed Operation” page 4-6 • • “Using Advanced Features (Options)” page 4-8 “Temperature Control”... -
Page 31: Switch On Centrifuge
Operation Switch on Centrifuge Switch on Centrifuge Note Make sure the centrifuge is properly installed before turning it ON for the first time, (see Chapter 2, Installation). 1. Turn on the power switch located on a small recessed panel in the upper right corner of the front cabinet panel into position “1“. -
Page 32: Rotor Installation
Operation Rotor Installation Door Gas Springs Periodically check that the two door gas springs are functioning properly. a. Open the chamber door and making sure it remains open. The gas springs counterbalance the weight of the door and hold it in the open position. If the chamber door will not stay in the open position, inform Customer Service. - Page 33 Operation Normal Operation 3. Confirm your entry by pressing ENTER. Your entry will also be automatically confirmed if you do not press any key for 5 seconds. Note The equivelant value will be calculated and displayed. If an extremely low RCF value has been selected, it will be corrected automatically if the resulting speed is less than 300 rpm.
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Page 34: Centrifugation
Operation Centrifugation Preselecting temperature You can preselect temperatures between -10 °C and +40 °C. 1. Press the TEMP key in order to enter the temperature. 2. Enter the desired temperature using the numeric pad. The digits show in sequential order. 3. -
Page 35: Programmed Operation
Operation Programmed Operation HOLD If you selected continuous operation (see “HOLD” page 4-4), you will have to stop the centrifuge manually. Press the STOP key on the control panel. The centrifuge will be decelerated at the designated rate. The message "END" will illuminate, and after pressing the key OPEN, the lid will open and you can remove the samples. - Page 36 Operation Programmed Operation 5. Enter a program number. 6. Press ENTER key. The SET DISPLAY will then return to the HOME screen, and that program number will appear in the PROG # field as confirmation: 7. Record saved information on the Program Log Pad (supplied). Note To prevent accidental erasure of established programs, if the entered program number has been previously assigned, the following message will appear: To review stored programs before overwriting, refer to your Program Log Pad or press RECALL.
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Page 37: Using Advanced Features (Options)
Operation Using Advanced Features (Options) Using Advanced Features (Options) The advanced features of the centrifuge allow for: • performing quality control runs, changing speed or time control ranges, • changing acceleration/deceleration or overtemperature alert settings, • linking together programmed run parameters, and •... - Page 38 Operation Using Advanced Features (Options) HOME SCREEN MENU START MENU 0 or ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER MENU 0 or ENTER ENTER Figure 4-1.
- Page 39 Operation Using Advanced Features (Options) SELECT QC RUN • QC RUN allows simple, automatic quality control run speed/ temperature verification when used with the optional computer interface package. The QC RUN feature will run and document the data from each set of parameters that has been saved in program memory. •...
- Page 40 Operation Using Advanced Features (Options) SLOW START • SLOW START chooses gentle acceleration from 0 to 250 rpm (acceleration transitions to the normal, maximum rate at 250 rpm), with the slow start rate defined by selection of one of ten different acceleration profiles.
- Page 41 Operation Using Advanced Features (Options) CHANGE OVERTEMPERATURE LIMIT • CHANGE OVERTEMPERATURE LIMIT allows changing the maximum allowable sample temperature to establish a new overtemperature offset value. • The centrifuge calculates the difference between the set and the maximum temperatures, and retains that value as an offset to apply to future runs, until it is changed.
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Page 42: Temperature Control
Operation Temperature Control • Changes to run parameters will be ignored unless STEP RUN is deselected before START is pressed. • The only changes that could be made without deselecting would be if a different step run was recalled from program memory (program numbers 10-15). •... -
Page 43: Rotor Temperature Equilibration
Operation Rotor Temperature Equilibration Rotor Temperature Equilibration To equilibrate the rotor temperature in the centrifuge chamber: 1. Install the rotor in the rotor chamber, place empty buckets (if applicable) in all positions, and the rotor cover, if any. 2. Close the door. 3. -
Page 44: Customer Control Inspection
Customer Control Inspection Contents “Speed Controls” page 5-2 • • “Timer Controls” page 5-2 “Temperature Controls” page 5-3 • Thermo Scientific RC B... -
Page 45: Speed Controls
Customer Control Inspection Speed Controls To keep your centrifuge in good working condition and ensure accurate test results, Thermo Fisher Scientific recommends that you check the speed controls, timer controls and temperature controls at least twice a year. If the bi-annual inspection reveals inaccurate results for any of these controls, inform Customer Service to recalibrate the controls. -
Page 46: Temperature Controls
Customer Control Inspection Temperature Controls Temperature Controls When verifying temperature control, all test run conditions (TEMP, SPEED, TIME, ROTOR and option selections) should match your required protocol(s). Test fluid must be at the same volume and temperature as the actual sample (use a calibrated thermometer to confirm). The sample temperature, rotor assembly temperature, and SET temperature must match also. -
Page 47: Maintenance And Care
Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-4 • • “Decontamination” page 6-5 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-6 Thermo Scientific RC B... -
Page 48: Cleaning Intervalls
Maintenance and Care Cleaning Intervalls Cleaning Intervalls For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis. Maintenance Recommended Interval Clean rotor chamber daily or when polluted Clean rotor daily or when polluted Accessories... - Page 49 Maintenance and Care Cleaning • The felt ring that seals the bearing openings must be fully soaked with oil to provide in a particular case a sufficient protection against leaking liquids. CAUTION Observe that no liquids, particularly no organic solvents, get into the openings at the motor shaft outlet, as they could leach out the grease of the bearings.
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Page 50: Disinfection
Maintenance and Care Disinfection Disinfection Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation. WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. -
Page 51: Decontamination
Maintenance and Care Decontamination Decontamination Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation. WARNING Radioactive material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. -
Page 52: Service Of Thermo Fisher Scientific
Maintenance and Care Service of Thermo Fisher Scientific 2. Place the rotor on a flat surface. • Rotors and adapter can beautoclaved at 121 °C. • The maximum permissible autoclave cycle is 20 minutes at 121 °C. Clean the rotor before autoclaving and rinse it with distilled water. Remove all accessories (tubes, adapters). -
Page 53: Troubleshooting
Troubleshooting Contents “Mechanical Emergency Door Release” page 7-2 • “Circuit Breakers” page 7-3 • “Failure message” page 7-3 • Thermo Scientific RC B... -
Page 54: Mechanical Emergency Door Release
Troubleshooting Mechanical Emergency Door Release Mechanical Emergency Door Release During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop. -
Page 55: Circuit Breakers
Troubleshooting Circuit Breakers Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the OPEN key to have the door locks operative again. Circuit Breakers The centrifuge has circuit breakers. In the event of a failure inform Customer Service. Failure message If problems occur other than those listed in this table, the authorized customer service representative must be contacted. - Page 56 Troubleshooting Failure message Failure message Problem with centrifuge Possible causes and cures AIR TEMP SENSOR The centrifuge cannot be operated. The temperatur sensor detects a temperature increase >10 °C, FAIL while the centrifuge is cooled down.. The cooling circuit is open. The temperature sensor is broken.
- Page 57 Troubleshooting Failure message Failure message Problem with centrifuge Possible causes and cures TACH FAULT The centrifuge cannot be operated. The tachometer signal is not available. DETECTED The emergency lid release enables you to retrieve your The run does not start or the centrifuge runs samples.
- Page 58 Troubleshooting Failure message Failure message Problem with centrifuge Possible causes and cures INTERNAL PROGRAM The centrifuge cannot be operated. The centrifuge detected a program fault. ERROR Restart the centrifuge. The run does not start or the centrifuge runs down without being braked. If an error message appears again, turn of the centrifuge.
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Page 59: Chemical Compatibility Chart
Chemical Compatibility Chart CHEMICAL 2-mercaptoethanol Acetaldehyde U U - M U U U M M - Acetone U U S U M S U U S U U U S U U S M M S Acetonitrile U M U U - M U U S ®... - Page 60 Chemical Compatibility Chart CHEMICAL Cesium Bromide Cesium Chloride Cesium Formate Cesium Iodide Cesium Sulfate Chloroform U U U U S M U S U U M U M U U U M M U U S U U U M S Chromic Acid (10%) U U S U U -...
- Page 61 Chemical Compatibility Chart CHEMICAL Hydrochloric Acid (conc.) U U U U - U U M - U M U U M U U U - U U U - Formaldehyde (40%) M M M S Glutaraldehyde Glycerol Guanidine Hydrochloride U U S ®...
- Page 62 Chemical Compatibility Chart CHEMICAL Lactic Acid (20%) N-Butyl Alcohol M M S N-Butyl Phthalate U U S U U U M - U U S M M S N, N-Dimethylformamide U U - U U S Sodium Borate Sodium Bromide Sodium Carbonate (2%) M U S Sodium Dodecyl Sulfate...
- Page 63 Chemical Compatibility Chart CHEMICAL Sucrose, Alkaline Sulfosalicylic Acid U U S Nitric Acid (10%) U U - Nitric Acid (50%) U M S U U - U U M M U M M M S Nitric Acid (95%) U U - U U - U U U U M U U U U M U U S Hydrochloric Acid (10%)
- Page 64 Chemical Compatibility Chart Satisfactory M = Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of use. U = Unsatisfactory, not recommended. Performance unknown; suggest testing, using sample to avoid loss of valuable material.
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Page 65: Warranty
Warranty Thermo Fisher Scientific makes no warranty of any kind, expressed or implied, except as stated in this warranty policy. The Thermo Scientific RC BIOS™ Centrifuge is warranted (subject to the conditions specified below and in the warranty clause of the Thermo Fisher Scientific terms and conditions of sale in effect at the time of sale) to be free from defects in material and workmanship for a period of one (1) year from the date of delivery. -
Page 66: Contact Information
Contact Information United States 866-9-THERMO +1 866 984 3766 Canada +1 866 984 3766 Austria +43 1 801 400 Belgium +32 2 482 30 30 Germany 08001 536 376 +49 6184 90 6940 France +33 2 2803 2180 Italy +39 02 02 95059 434-254-375 Netherlands +31 76 571 4440 Nordic / Baltic Countries... - Page 67 Contact Information Spain +34 932 23 09 18 Portugal +34 932 23 09 18 Switzerland +41 44 454 12 12 UK / Ireland +44 870 609 9203 China +86 21 6865 4588 +86 10 8419 3588 India 1800 22 8374 +91 22 6716 2200 Japan +81 45 453 9220...
- Page 68 Index Accident Prevention .......... iv Mains Connection ...........2-5 Aligning the Centrifuge ........2-5 Maintenance ...........6-1 Autoclaving ............. 6-5 Operation ............4-1 Care ..............6-1 Centrifuging ............ 4-5 Cleaning ............6-2 Precautions ............iv Cleaning Agents ..........6-3 Preface ............... iii Close Lid ............4-2 Preselecting RCF ..........4-3 Contact Information ........
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