Thermo Scientific NanoDrop Lite Plus User Manual page 12

1 NanoDrop Lite Plus User Guide
Nucleic Acid Measurements
12 NanoDrop Lite Plus User Guide
c = (A *CF)/b
where,
c = the nucleic acid concentration in ng/μL
A = the sample absorbance
CF = the conversion factor in ng-cm/μL
b = the pathlength in cm
The generally accepted conversion factors for nucleic acids are:
• Double-stranded DNA: 50 ng-cm/μL
• Single-stranded DNA: 33 ng-cm/μL
• RNA: 40 ng-cm/μL
Table 3.Nucleic acid concentration range
Lower
Sample
Detection
Type
Limit
dsDNA 2.0 ng/μL
ssDNA 1.3 ng/μL
RNA 1.6 ng/μL
Note Absorbance reported is normalized to a 1.0 cm (10.0 mm) pathlength for
all measurements.
Upper Typical reproducibility;
Detection based on 10 replicates
Limit (SD = ng/µL; CV=%)
1500 ng/μL ±2.0 ng/μL for sample
concentrations between 2.0 and
100 ng/μL samples; ±2% for
samples >100 ng/μL
990 ng/μL ±2.0 ng/μL for sample
concentrations between 2.0 and
100 ng/μL samples; ±2% for
samples >100 ng/μL
1200 ng/μL ±2.0 ng/μL for sample
concentrations between 2.0 and
100 ng/μL samples; ±2% for
samples >100 ng/μL
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