Protein Measurement Screen; Protein Calculations - Thermo Scientific NanoDrop Lite Plus User Manual

1 NanoDrop Lite Plus User Guide
Protein Measurements

Protein Measurement Screen

Protein Calculations

14 NanoDrop Lite Plus User Guide
Application
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Sample measurement screen
Proteins, unlike nucleic acids, exhibit considerable diversity. The Protein A280
application is indicated for purified proteins that contain Trp, Tyr residues or Cys-Cys
disulphide bonds and exhibit absorbance at 280 nm. The Protein A280 application
measures sample absorbance at 280 nm (A280) and calculates the concentration
(mg/mL) using the selected extinction coefficient. The Protein A280 application does
not require generation of a standard curve.
The NanoDrop Lite Plus should be used to measure the concentration of purified
protein samples. Direct protein concentration measurements assume a highly
purified sample as all absorbance at 280 nm is included in the calculation of the
protein concentration. Carryover of starting materials, purifying reagents, and cell
material into the measured protein sample will result in an artificially high protein
concentration.
Sample name
Sample measurement data
Measure sample
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solution
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