Nucleic Acid Measurement Screen; Nucleic Acid Calculations - Thermo Scientific NanoDrop Lite Plus User Manual

Nucleic Acid Measurement Screen

Nucleic Acid Calculations

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The NanoDrop Lite Plus should be used to measure the concentration of purified
nucleic acid samples. Direct nucleic acid concentration measurements assume a
purified sample as all absorbance at 260 nm is included in the calculation of the
nucleic acid concentration. Carryover of nucleotides, primers, purifying reagents,
and cell material into the measured nucleic acid sample will overestimate the nucleic
acid concentration.
For nucleic acid quantification, the Beer-Lambert equation is modified to use a
conversion factor with units of ng-cm/μL. The modified equation used for nucleic
acid calculations is the following:
Sample name
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Sample measurement screen
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NanoDrop Lite Plus User Guide
Nucleic Acid Measurements
Sample measurement data
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