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Thermo Scientific CryoStar NX70 Operator's Manual

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Thermo Scientific
CryoStar NX70 / NX50
Operator Guide
387928

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Summary of Contents for Thermo Scientific CryoStar NX70

  • Page 1 Thermo Scientific CryoStar NX70 / NX50 Operator Guide 387928...
  • Page 2 The development of Thermo Scientific products and services is an ongoing process. Please ensure that any published information you use as a reference is up to date and relates to the condition of the product.
  • Page 3 Important Note Regarding Instrument Variants This document is designed for use with the Thermo Scientific CryoStar NX70 and NX50 cryostats. A range of optional features is available on these instruments and the instructions contained in this manual are not specific to any one variant. Thus some features described may not be available on your instrument.
  • Page 5 Symbols The following symbols and conventions may be used throughout this document and on the instrument: This symbol is used on the equipment, or in a document, to indicate that instructions must be followed for safe and correct operation. If this symbol appears on the instrument, always refer to the operator guide.

  • Page 7: Table Of Contents

    Table of Contents 1 - Safety Information ............................. 1  2 - Introduction.............................. 5  Introduction to the CryoStar NX70 / NX50 ....................5  Intended Use ............................5  Instrument Features ..........................5  Technical Specifications ..........................6  3 - Instrument Setup............................9  Unpacking and Repacking .......................... 9 ...
  • Page 8 Draft CryoStar 2011-06-10 Height Adjustment ..........................48  Multi Function Bar ..........................49  Time and Date ............................. 49  Cutting Mode Selection ........................49  Handwheel Brake..........................56  Multi-Function Button ......................... 57  Menu ..............................59  Chamber Light ............................. 59  Standby ..............................60  Display Lock ............................61  Favorites ...............................
  • Page 9 Table of Contents Handwheel Brakes ............................ 91  Electronic Handwheel Brake........................91  Mechanical Handwheel Brake........................ 92  Cold D ..............................94  Starting the Disinfection (Cold D) Function ..................94  Aborting the Disinfection ........................95  Programming Cold D ........................... 96  5 - Troubleshooting ............................99  Troubleshooting Tables ..........................

  • Page 11: Safety Information

    1 - Safety Information Introduction Thermo Fisher Scientific products are designed for convenient and reliable service; however, incorrect actions by a user may damage the equipment, or cause a hazard to health. The following sections contain important information for the safe setup and use of the instrument. All users must read and understand the following sections before using the instrument.
  • Page 12  It is important that normal standards of safety and good laboratory practices are employed. Always use common sense when operating the instrument.  Any problems and queries should be referred to your Thermo Fisher Scientific supplier.  Correct maintenance procedures are essential for consistent performance. It is recommended that a Maintenance Contract is taken out with our Service Department.
  • Page 13 1 - Safety Information Environment This product is required to comply with the European Union's Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling / disposal companies in each EU Member State, and this product should be disposed of or recycled through them.
  • Page 14 Product Return Safety Declaration...

  • Page 15: Introduction

    Introduction to the CryoStar NX70 / NX50 Intended Use The Thermo Scientific CryoStar NX70 and NX50 are high-performance cryostats designed to prepare tissue sections of frozen samples in medical, pharmaceutical and research laboratories. Only trained laboratory personnel may operate the CryoStar NX70 / NX50.

  • Page 16: Technical Specifications

    Technical Specifications Mechanical Specifications Depth 100 cm (39.4 in) Width 75.5 cm (29.7 in) Height Range Adjustable range 82 - 112 cm (32.3 - 44.0 in) Maximum Weight 200 kg (440 lb) Electrical Specifications Power Requirements Dedicated line recommended. 100V, 50/60Hz, 10A, +/-10%, 110-120V, 60Hz, 10A, +/-10% 220-240V, 50/60Hz, 5A, +/-10% Environmental Specifications For Indoor Use Only...
  • Page 17 2 - Introduction Cooling Specifications Specimen Temperature Control -50°C to +10°C (-58°F to +50°F) at +20°C room Temp. (decrease 0,7°C/1°C room temp. increase) Blade Temperature Control -35°C to -5°C (-31°F to +23°F) at 20°C room Temp (decrease 0,5°C 71°C room temp. increase) Cryobar Cooling Max.
  • Page 18 Cold D Specification Reservoir Volume 1000 ml Recommended Medium Sanosil S010 (5% H2O2) ® Default Settings Counter Mode Speed V = 0 Multi Counter Operating Mode Continuous Retraction Language English Fine 0.5 μm Trim 5 μm Joystick Calibration Calibrated Chamber Light 100% Screen Brightness 100%...

  • Page 19: Instrument Setup

    3 - Instrument Setup Unpacking and Repacking Safety Precautions The CryoStar is heavy and requires a minimum of two people to safely unpack and maneuver it. The CryoStar has a high centre of gravity and care should be taken when moving. Do not tip the CryoStar more than 30°...
  • Page 20  The instrument can be moved on its castors. However, ensure that it does not roll off the pallet inadvertently.  Screw in the two bails (part of the standard accessories) into the respective holes in the foot of the cryostat. ...

  • Page 21: Location & Setup

    3 - Instrument Setup Location & Setup When deciding where to locate the CryoStar NX70 / NX50, the following items must be taken into consideration:  Approximately 10 cm (4 in) must be allowed around the cooling system to allow free air flow.

  • Page 22: Identification Of Parts

    Identification of Parts Overview Cryochamber Connections Panel Cryochamber Window Handwheel Touch Screen Handwheel Mechanical Brake Cold D Reservoir Cover Water Drain Reservoir Cover Emergency Stop Button...
  • Page 23 3 - Instrument Setup Connections Panel Power Switch X1 – RJ45 port (for Service purposes only) Power Socket X2 – USB port Rating and Serial Number Label X3 – Foot Pedal Connector...
  • Page 24 Cryochamber Cryobar and Cooled Storage Area Cutting Speed Control Motorized Cutting Start / Stop Trim Button Button Joystick Handwheel Electronic Brake Button Specimen Head Vacutome Filter Cover Vacutome Filter Cover Release Blade Holder with Anti-Roll Plate Knob Vacutome Section Waste Tray Mode Selection Button...
  • Page 25 3 - Instrument Setup Blade Holder Lateral Adjustment Lever Blade Holder Base Anti-Roll Plate Anti-Roll Plate Adjustment Blade Clamping Lever Clearance Angle Adjusting Screw Blade Holder Base...
  • Page 26 Specimen Head NX70 Y-axis Fine Adjustment Knob Specimen Head Clamping Lever Upper Specimen Chuck Jaw Specimen Chuck Release Lever (Static) Lower Specimen Chuck Jaw X-Axis Fine Adjustment Knob (Moving)
  • Page 27 3 - Instrument Setup Specimen Head NX50 Upper Specimen Chuck Jaw Specimen Chuck Release Lever (Static) Lower Specimen Chuck Jaw Orientation Handle (Moving) Specimen Head Clamping Lever...

  • Page 28: Set Up

    Set Up Before sectioning, the blade holder and the cryo chamber should be at a stable temperature close to the desired cutting temperature. These temperatures should be selected to suit the consistency of the specimen. All tools which come into contact with the sections or which are used to manipulate the specimen must also be cooled to prevent the tissue from sticking to them.

  • Page 29: Fitting Or Exchanging Low Profile Blades

    3 - Instrument Setup Fitting or Exchanging Low Profile Blades ICROTOME BLADES ARE EXTREMELY SHARP AND CAN CAUSE SEVERE INJURIES ALWAYS USE GOOD LABORATORY PRACTICE WHEN HANDLING THEM LWAYS COVER THE BLADE WITH THE KNIFE BLADE GUARD AND ACTIVATE THE BRAKES PRIOR TO MAKE MODIFICATIONS TO THE BLADE KNIFE AND SPECIMEN OR CHANGING THE SPECIMEN The blade holder should be fitted with low profile blades (80mm x 8mm;...
  • Page 30  Carefully slide a low profile blade into the slot.  Push the blade clamping lever towards the back of the instrument to clamp the blade in position.

  • Page 31: Removing Used Blades With The Magnetic Blade Tool

    3 - Instrument Setup Removing Used Blades with the Magnetic Blade Tool The magnetic blade tool allows the user to remove used blades from the blade holder in a save way. Remove the used blade with the magnetic blade tool: ...

  • Page 32: Blade Positioning And Orientation

    Blade Positioning and Orientation LWAYS COVER THE BLADE WITH THE KNIFE BLADE GUARD AND ACTIVATE THE BRAKE PRIOR TO MAKE MODIFICATIONS TO THE BLADE KNIFE If the cutting area of the blade is no longer usable, the upper part of the blade holder can be moved without removing the blade.

  • Page 33: Adjusting The Cutting Angle

    Move the lateral adjustment lever forward to secure the upper part of the blade holder in the new position. Adjusting the Cutting Angle The cutting angle can be adjusted. the recommended cutting angle is approximately 10° for Thermo Scientific low profile blades.
  • Page 34  Gently push both sides of the blade holder base to select the desired angle. WARNING: T HE SURFACE OF THE BLADE HOLDER BASE MIGHT BE COLD SE GLOVES IF NECESSARY Note The clearance angle can be read on the scale on the left side on the upper part of the blade holder. Note 10°...

  • Page 35: Adjusting The Anti-Roll Plate

    3 - Instrument Setup Adjusting the Anti-Roll Plate The fine adjustment of the anti-roll plate is carried out using the knurled screw.  To raise the height of the Anti Roll Plate, turn the screw anti clockwise.  To lower the height of the Anti Roll Plate, turn the screw clockwise. Note Always adjust the height of the Anti Roll Plate in small increments.

  • Page 36: Specimen Setup

    Specimen Setup Specimen Orientation NX70 Before sectioning can begin, the specimen must be correctly clamped and oriented in the specimen head. To clamp and orient the specimen:  Loosen the specimen chuck release lever and insert the specimen chuck into the jaws of the specimen head.
  • Page 37 3 - Instrument Setup  Use the fine adjusting knobs to orient the specimen on the x- and y-axes. Note The fine adjustment knobs will click into place when the center position has been reached.  Tighten the specimen head clamping lever again. For further specimen orientation, the specimen chuck can also be rotated by 360°...

  • Page 38: Specimen Orientation Nx50

     Tilt the joystick forwards to move the blade holder towards the specimen. Note Tilting the joystick further forwards will increase the speed of approach. WARNING: T O AVOID SEVERE DAMAGE ENSURE THAT THE BLADE DOES NOT CRASH INTO THE SPECIMEN Specimen Orientation NX50 Before sectioning can begin, the specimen must be correctly clamped and oriented in the specimen head.
  • Page 39 3 - Instrument Setup  Release the specimen chuck release lever again to allow the jaws to grip the chuck.  Loosen the specimen head clamping lever to allow adjustment of the specimen parallel to the cutting edge.  Use the orientation handle to orient the specimen on the x- and y-axes. Note The orientation handle will click into place when the center position has been reached.
  • Page 40  Tighten the specimen head clamping lever again. For further specimen orientation, the specimen chuck can also be rotated by 360° as described below:  Loosen the specimen head clamping lever slightly.  Rotate the chuck as required.  Move the specimen head clamping lever back to its original position. ...

  • Page 41: Filling With Disinfectant To Perform The Cold D Function

    3 - Instrument Setup Filling with Disinfectant to Perform the Cold D Function The Cold D function is an integrated system for the delivery of disinfectant (Sanosil®) into the cold cryostat chamber. This method of disinfection ensures an even distribution of the disinfectant throughout the cryo-chamber. The Cold D cycle takes 50 minutes to complete and the instrument cannot be used during this time;...
  • Page 42  Remove the fitting on top of the bottle.  Empty the contents into the tank.  Pull out the empty bottle and close the reservoir cover. Notes The maximum capacity of the disinfectant tank is 1 liter, which is sufficient for approximately 18 Cold D cycles. If the fluid level in the disinfectant tank is too low, an error message will be shown on the touch screen display.

  • Page 43: Operation

    4 - Operation Switching On WARNING: B EFORE SWITCHING ON THE INSTRUMENT FOR THE FIRST TIME ENSURE THAT THE POWER REQUIREMENTS INDICATED ON THE RATING LABEL CORRESPOND TO THE POWER SUPPLY VOLTAGE BEING USED Notes No other instruments should be connected to the circuit used for the cryostat, as the compressor needs high surge currents when started.

  • Page 44: System Interfacing

    System Interfacing Using the Touchscreen The CryoStar NX70 / NX50 has an embedded touchscreen user interface which is used to set all preferences. WARNING: A VOID USING SHARP OR POINTED OBJECTS TO PRESS BUTTONS ON THE TOUCHSCREEN Main Screen The Main screen appears after the instrument has been switched on.
  • Page 45 4 - Operation  Press the required key to activate / deactivate the function or to display the options screen for the selected function - See Setting Preferences. "OK" Button  Press the "OK" button to save any changes to specific settings. "Back"...

  • Page 46: Setting Preferences

    Setting Preferences Quick Freezing of the Specimen The cryobar provides on-demand, quick freezing of specimens. Notes When the cryobar is activated, it will take approximately 2 - 3 minutes for the cryobar to reach a temperature of -60°C. The cryobar remains active for approximately 10 minutes. The specimen is frozen onto the specimen chuck using a freezing compound and the cryobar.

  • Page 47: Trimming And Fine Section Thickness

    4 - Operation Trimming and Fine Section Thickness The trimming and fine section thickness can be set in two ways: Using the joystick  Tilt the joystick either to the left or to the right to toggle between "Fine" and "Trimming" thickness settings.
  • Page 48 Using the touch screen display To set the trimming section thickness:  Press the "Trimming Thickness" button on the Main screen.  The Trim Section screen will be displayed.  Either select one of the preset values (400μm, 200μm, 100μm or 10μm) or adjust the value using the arrow keys.

  • Page 49: Temperature

    4 - Operation  Either select one of the preset values (100μm, 50μm, 10μm or 5μm) or adjust the value using the arrow keys.  For details of the thickness steps see Technical Specifications  Press "OK" to confirm the setting and to return to the Main screen. ...
  • Page 50  Press the "Back" button to exit back to the Main screen without saving the changes. Note The new set temperature is displayed in white on the Main screen until the temperature is reached. Set the blade temperature  Press the "Blade Temperature" button on the Main screen. ...

  • Page 51: Cutting Window

    4 - Operation Cutting Window The cutting window allows the user to customise this setting for each specimen.  On the Main screen press the "Cutting Window" button.  The Set Cutting Window screen will be displayed.
  • Page 52 To set the lower limit of the cutting window  Turn the handwheel so that the lower edge of the specimen is positioned slightly above the blade edge.  Press the “Start" button.  The lower limit should now be set and the "Start" button is highlighted in yellow. To set the upper limit of the cutting window ...

  • Page 53: Select A Favorite

    4 - Operation Select a Favorite Favorites are user-definable, pre-selectable temperature settings for the specimen head and the blade holder. To select one of the preset Favorites:  Press the "Favorites" button on the Main screen.  The Favorites sliding menu will appear. ...
  • Page 54  Press the "Vacutome" button on the Main screen.  The Vacutome screen will be displayed. Activate the Vacutome To activate the Vacutome:  Press "Vacutome" to display the Vacutome settings screen.  Use the arrow keys to increase or decrease the Vacutome suction value - the default setting is 100%.
  • Page 55 4 - Operation  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button to exit back to the Main screen without saving the changes. Note The Vacutome suction setting can also be adjusted by turning the Joystick as long as the Vacutome screen is displayed. ...
  • Page 56 Turning On the cleaning mode To activate the cleaning mode:  Press the "Vacutome" button on the Main screen.  The Vacutome screen will be displayed.  Press "Cleaning Mode" to activate this mode. Notes The "Cleaning Mode" button turns yellow to indicate that this function is now activated. The cleaning mode will switch of automatically after 10 minutes.
  • Page 57 4 - Operation Turning Off the cleaning mode To deactivate the cleaning mode:  Press the yellow "Vacutome" button on the Main screen.  Press the yellow "Cleaning Mode" button on the Vacutome settings screen - the button will now appear white.

  • Page 58: Height Adjustment

    Height Adjustment The CryoStar NX70 / NX50 cryostat has an electronic height adjustment which enables the user to raise or lower the cryostat to a convenient working height. WARNING: Do not lean on the instrument when the height adjustment is carried out to avoid damage to the instrument.

  • Page 59: Multi Function Bar

    4 - Operation Multi Function Bar The multi function bar provides the user access to useful information and functions. The Multi Function Bar is located at bottom of the Main screen. Time / Date / Service Message Multifunction Button Cutting Mode Brake Time and Date To toggle between Time or Date indication:...
  • Page 60  Single  Interval  Multi-Stroke  Continuous-Stroke Note In case of emergency, the motor drive can be stopped in every operating mode by pressing the emergency stop button or the emergency stop in the (optional) foot pedal. To continue sectioning pull out the emergency stop button or unlock the emergency stop in the foot pedal.
  • Page 61 4 - Operation  The Menu screen will be displayed.  Press the "Cutting Mode" button.  The Menu: Cutting Mode screen will be displayed.  Press on the desired cutting mode (Continuous, Interval, Single or Multi).  Press "OK" to confirm the setting and to return to the Main screen. ...
  • Page 62 To switch between the cutting modes using the "Mode Selection" button on the right touch panel:  Press the mode selection button on the left touch panel.  The actual cutting mode is displayed on the multi function bar on the Main screen. Mode: Single By using this mode, a single full cutting movement is carried out with the specimen head stopping in the down position.
  • Page 63 4 - Operation Mode: Interval The cutting movement is carried out as long as the Start/Stop button or the foot pedal is pressed. The movement can be stopped in any position.  Press either the "Start/Stop" button twice or press the foot pedal twice. ...
  • Page 64  Press the "Cutting Mode" button.  The Menu: Cutting Mode screen will be displayed.  Press the "Multi" button to highlight it.  Use the arrow keys to select the number of strokes.
  • Page 65 4 - Operation Notes The default number of cutting strokes is 2. The minimum number of strokes is 2, the maximum number is 99.  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button twice to exit back to the Main screen without saving the changes. To carry out Multi-Stroke sectioning: ...

  • Page 66: Handwheel Brake

    Handwheel Brake WARNING: A LWAYS ACTIVATE THE BRAKE WHEN MANIPULATING THE BLADE KNIFE AND SPECIMEN To activate the Handwheel Brake:  Press the "Brake" button on the Main screen or press the "Brake" button on the right touch pad. Note The "Brake"...

  • Page 67: Multi-Function Button

    4 - Operation Multi-Function Button The Multi-Function button provides to the user additional information on the sectioning process.  Four different indications can be displayed by pressing this button. Remaining Distance to the Front End Position Indicates the remaining distance to front end position of the blade holder. Section Thickness Sum This button indicates the total section thickness sum of the actual mounted specimen.
  • Page 68 Motorized Cutting Drive The motorized cutting drive facilitates routine work and ensures a steady cutting speed even for hard specimens. When using the motorized cutting drive the hand wheel is controlled automatically. Setting the Cutting Speed To set the speed at which motorized cutting drive will operate: ...

  • Page 69: Menu

    4 - Operation Section Counter The section counter adds up the number of the produced sections. After each downward movement of the specimen head, the number on the section counter increases by 1. To reset the section counter:  Press the Section Counter button for approximately 3 seconds to reset it to zero. Menu The "Menu"...

  • Page 70: Standby

    The chamber illumination can be adjusted; from the lowest (1%) to the highest (100%) level. Standby When the CryoStar NX70 is switched on but has been inactive for 1 hour, it will automatically enter the Standby mode. After 3 more hours, the instrument enters the sleep mode. The cryostat maintains the user adjusted temperatures for both blade holder and specimen head during Standby.

  • Page 71: Display Lock

    4 - Operation To enter the Standby-mode:  Press the "Menu" button on the Main screen.  The Menu screen will appear.  Press the "Standby" button to switch the instrument immediately into the Standby mode.  Press the touchscreen to exit the Standby or Sleep mode. Display Lock Note: The display lock is used to avoid unwanted changes to the settings of the cryostat.

  • Page 72: Favorites

     Press “Display Lock” to activate the display lock immediately. To deactivate the Display Lock:  Press the "Lock" Symbol three times. Note The symbol changes its color (red - yellow - green) each time it is pressed. Favorites Favorites are a set of preset values for the specimen head and blade temperature. The user can program three favorites to allow quick selection of the set of temperatures most often used.
  • Page 73 4 - Operation Preset 3 Title Box Specimen Head Temperature Preset 2 Title Box Blade Temperature Preset 1 Title Box Alternative Indication Each favorite setting is divided into four sections (from left to right):  Favorite name (displayed on the main screen if highlighted). ...

  • Page 74: User Guide

     Erase letters or numbers by pressing the "Delete" button.  Type in the new name for the favorite (for example “Muscle” / “Fat” / “Bone”).  Press "OK" to confirm the setting and to return to the Favorites menu. ...

  • Page 75: Settings Menu

    4 - Operation  Use the scroll bar on the right side or the two arrow keys to scroll through the manual.  Press the "Home" button to return to the first page of the User Guide.  Use the arrow keys to browse forward or backwards through the User Guide. ...
  • Page 76  The menu screen will appear.  Press the "Settings" button.  The Menu: Settings screen will appear.  Press the "Back" button to return to the Menu screen.  Press the "Back" button twice to return to the Main screen.

  • Page 77: Language

    4 - Operation Language To set the Language:  Press the "Menu" button on the Main screen.  The menu screen will appear.  Press the "Settings" button.  The Menu: Settings screen will appear.  Press the "Language" button. ...

  • Page 78: Time And Date

    Time and Date Note If a Defrost or Cold D cycle is running, the cycle must finish before the time or date can be adjusted.  Press the "Menu" button on the Main screen.  The Menu screen will be displayed. ...
  • Page 79 4 - Operation Set the Day  Press the "Day" button to highlight it.  Use the arrow keys to select the desired day and date. Set the Month  Press the "Month" button to highlight it.  Use the arrow keys to select the desired month. Set the Year ...
  • Page 80 Change to ISO format  Press the "ISO" button to toggle between the full date format and the ISO format.  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button three times to exit back to the Main screen without saving the changes. Setting the Time ...
  • Page 81 4 - Operation  Press the "Time" button.  The Menu: Settings: Time screen will be displayed. Set the minutes  Press the "Minute" button to highlight it.  Use the arrow keys to select the desired minute. Set the hour ...

  • Page 82: Start Time

    Note The 12/24 button is highlighted in yellow if the 12h mode is active.  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button three times to exit back to the Main screen without saving the changes. Start Time The Start Time is the time were the cryostat switches from the sleep mode to the Active mode on every programmed day.
  • Page 83 4 - Operation  The Menu: Settings: Start Time screen will appear.  In this menu, the user can specify the day(s) and the exact time when the cryostat should start automatically. Note The preset start time is from Monday to Sunday at 06:00 a.m. To set the Day: ...
  • Page 84  Press the weekdays on which the cryostat should not start automatically. Note Deactivated weekdays will turn white.  Press the "Hour“ and “Minute” button and use the arrow keys to adjust the time at which the cryostat should start. ...

  • Page 85: Defrost

    4 - Operation Defrost Inevitably, when working on the microtome the dry cold air of the cryo chamber mixes with the warm humid air outside the chamber. Frost forms on the finned evaporator. The increasing thickness of the frost reduces the efficiency of the evaporator.
  • Page 86  Press the "Defrost" button.  The Menu: Settings: Defrost screen will be displayed.  Press the "Back" button three times to return to the Main screen without starting the defrosting.  Press the "Now" button to start the defrosting immediately. ...
  • Page 87 4 - Operation To abort the defrosting:  Press the "Abort" button on the Defrosting progress screen.  The Main screen will appear, displaying an error message.  Press the "OK" button to remove the error message. Note The Defrosting cycle can be aborted at any time.

  • Page 88: Programming The Daily Defrost Cycle

    Programming the Daily Defrost Cycle Note The time for the daily defrost cycle should be programmed outside of the normal working hours to avoid delays in the workflow. To program the Daily Defrost Cycle:  Press the "Menu" button on the Main screen. ...

  • Page 89: Screen

    4 - Operation  The Menu: Settings: Defrost-Time screen will appear. Note The standard setting for the daily defrost cycle is daily at 00:00 a.m..  To change the standard setting, press the "Hour" and / or the "Minute" button. ...
  • Page 90  The Menu: Settings screen will appear.  Press the "Screen" button.  The Menu: Settings: Screen screen will be displayed.  Select one of the preset values (25%, 50%, 75% or 100%) or adjust the brightness using the arrow keys. ...

  • Page 91: Retraction

    4 - Operation Retraction If required, the Retraction function can be turned off. To toggle the retraction On or Off:  Press the "Menu" button on the Main screen.  The Menu screen will be displayed.  Press the "Settings" button. ...

  • Page 92: Data Log

     Press the OK" button on the Menu: Settings: Retraction screen to turn on the Retraction and to return to the Main screen.  Press the "Back" button three times to return to the Main screen without saving the changes. Data Log The Data Log records all events and error messages on the cryostat.

  • Page 93: Default Settings

    4 - Operation  Press the "Settings" button.  The Menu: Settings screen will appear.  Press the "Data Log" button.  The Menu: Settings: Data Log screen will be displayed.  Use the scroll bar on the right side to scroll through the Data Log. ...
  • Page 94  Press the "Menu" button on the Main screen.  The Menu screen will be displayed.  Press the "Settings" button.  The Menu: Settings screen will appear.  Press the "Default Settings" button.  The Menu: Settings: Default Settings screen will be displayed. ...

  • Page 95: Service

    4 - Operation Display icons with text The text underneath the Main screen icons can be switched off if required. To switch the text off:  Enter the Menu: Settings: Default Settings screen as described above.  Uncheck the box and press the "Back" button three times to save the changes and to return to the Main screen.

  • Page 96: Emergency Stop

    Emergency Stop Standard Emergency Stop Function To activate the emergency stop:  Press the red emergency stop button to immediately stop the motorized cutting drive. Note When the emergency stop is activated, the handwheel brake button in the display and the handwheel brake button on the right keypad light up and a red button is shown on the display.

  • Page 97: Cooling Processes

    4 - Operation Cooling Processes Specimen and blade holder cooling Integrated refrigeration systems allow the blade holder to be cooled to -35°C and the specimen head to be cooled to -50°C. Specimen stations are located on the left side inside the cryo chamber. These specimen stations passively cool specimens to a temperature of -30°C and can be used as a staging area.

  • Page 98: Sectioning Procedures

    Sectioning Procedures Cutting Movement and Retraction Front Rear Lateral view into instrument Notes The retraction prevents the surface of the specimen being damaged during the return stroke of the specimen head. The retraction can be switched off - see "Switching the Retraction On - Off". The retraction distance required is 20 microns and cannot be changed.

  • Page 99: Trimming

    4 - Operation Trimming  Make sure that the trimming thickness setting is selected by using the joystick. Note Trimming thickness is shown highlighted on the display.  Hold down the "Trim" button and turn the hand-wheel in a clockwise direction to begin trimming. Note The blade holder will move forwards by the preset trim thickness value every cycle.
  • Page 100  Turn the hand wheel in a clockwise direction or use the motorized cutting drive to carry out sectioning. Note The section slides into the space between the blade and the anti-roll plate.  Use the handle to move the anti-roll plate assembly away from the specimen. ...

  • Page 101: Handwheel Brakes

    4 - Operation Handwheel Brakes The cryostat is equipped with an electronic and a mechanical handwheel brake to prevent unintended movement of the specimen head. Activating the brakes reduces the risk of being injured when adjusting the specimen head and/or the blade carrier. WARNINGS: W HEN THE INSTRUMENT IS TURNED OFF THE ELECTRONIC...

  • Page 102: Mechanical Handwheel Brake

    Mechanical Handwheel Brake The mechanical handwheel brake acts as an additional safety device, especially when the instrument is turned off and the electronic handwheel brake is inoperable. To mechanical handwheel brake can be activated by a switch on the handwheel. To activate the mechanical Handwheel Brake ...
  • Page 103 4 - Operation Notes If the switch is not in the correct position to lock, keep the switch pressed and move the handwheel slowly forward and backwards until the switch is locked. Starting the cutting motor drive is not possible when the instrument is turned off or when the mechanical hand wheel brake is activated.

  • Page 104: Cold D

    Cold D Starting the Disinfection (Cold D) Function The Cold D function allows the user to disinfect the cryo chamber with Sanosil®. Disinfection is either on- demand or pre-programmed. The Cold D cycle takes 50 minutes to complete. HE WINDOW OF THE CRYO CHAMBER MUST ALWAYS BE CLOSED BEFORE STARTING AND DURING A CYCLE Note:...

  • Page 105: Aborting The Disinfection

    4 - Operation Note Pressing the "OK" button starts immediate disinfection of the cryostat chamber, regardless of the programmed times.  The Disinfection Remaining Time screen will appear. Notes After the disinfection cycle is completed, the Disinfection Remaining Time screen will disappear and the Main screen will be displayed.

  • Page 106: Programming Cold D

    Note If the Disinfection cycle was aborted, an error message appears on the Main screen.  Press the "OK" button to remove the error message. Note An interrupted Cold D cycle will not adequately disinfect the cryo chamber. However, the instrument can be used immediately afterwards.
  • Page 107 4 - Operation  Press the "Disinfection“ button.  The Menu: Settings: Disinfection screen will appear.  In this menu, the user can specify the day(s) and the specific time when the Cold D process should automatically be carried out. To set the Day: ...
  • Page 108 Note Deactivated weekdays will turn white.  Press the "Hour“ and “Minute” button and use the arrow keys to adjust the time at which the Cold D cycle should start.  When all settings have been done, press “OK” to save the settings and to return to the Main screen. ...

  • Page 109: Troubleshooting

    5 - Troubleshooting Troubleshooting Tables Problem Possible Reason Advice Sections fold or 1. Specimen is too warm. Lower specimen temperature crumple 2. Knife holder too warm. Lower chamber temperature. Tip – use freezer spray on clamp plate and blade to confirm. 3.
  • Page 110 Problem Possible Reason Advice 3. Specimen incorrectly Check specimen is securely mounted and clamped. clamped in specimen head jaws. Check there is no debris or ice on the back of the chuck or on specimen head. Sections thick-thin 1. Check knife holder and specimen correctly clamped.

  • Page 111: Tips For Successful Sectioning

    5 - Troubleshooting Tips for Successful Sectioning For optimal sectioning, the following points should be followed:  Check the condition of the blade edge; move it horizontally to the left or right side to obtain a sharp cutting edge.  Check adjustment of anti-roll plate and correct it, if necessary.

  • Page 112: Optimum Temperature Ranges For Sectioning Different Types Of Fresh Specimen

    Optimum Temperature Ranges for Sectioning Different Types of Fresh Specimen Specimen Type Knife/Blade Temp Specimen Temp Adipose Tissue -35°C to -40°C -30°C or below Bone Marrow -25°C to -30°C -20°C Brain -20°C -12°C Breast -35°C -25°C Breast with Fat -35°C to -40°C -30°C or below Cervix -25°C to -30°C...

  • Page 113: Cleaning And Maintenance

    6 - Cleaning and Maintenance Cleaning the Touch Screen Display  Use commercially available cleaning tissues or use a soft and slightly wet towel to clean the Touch screen. WARNING: D O NOT USE HOUSEHOLD CLEANER OR CHEMICALS YLENE FOR CLEANING OF THE OUCH SCREEN TO AVOID SEVERE DAMAGE Shutting Off for Cleaning...

  • Page 114: Emptying The Defrosting And Cold D Liquids

    Emptying the Defrosting and Cold D Liquids The liquids from the daily defrosting cycle as well as the liquid of a Cold D cycle is collected in a bottle, located in the base of the cryostat. HE LIQUIDS FROM THE DAILY DEFROST AND CYCLE MIGHT BE HAZARDOUS EAR PROTECTIVE CLOTHING...

  • Page 115: Exchange The Vacutome Filter Bag

    Index Exchange the Vacutome Filter Bag. The Vacutome Filter Bag collects all section waste which has been removed with the vacutome function. WARNING: Section waste can be hazardous! Dispose of the used vacutome filter bag in accordance to the laboratory regulations. The Vacutome Filter Bag is placed under the cover on the right hand site of the cryo chamber.
  • Page 116  Clean the vacutome hose with a flexible brush.  Slip off the old vacutome filter and replace it by a new one.  Clean and disinfect the empty vacutome filter chamber with disinfectant.  Replace the cover, rear end first. ...

  • Page 117: Adjustment Of The Movable Specimen Head Jaw

    Index Adjustment of the Movable Specimen Head Jaw The clamping mechanism can be adjusted in case the chucks cannot be clamped properly.  Lock the handwheel brake.  Turn the adjusting ring by using the pin, provided with the standard accessories. ...

  • Page 118: Transportation Of The Instrument

    WARNING: A NY TRANSPORTATION OF THE INSTRUMENT REQUIRES ORIGINAL PACKAGING MATERIALS Note If the original packaging is no longer available, please contact your local Thermo Scientific representation.  Clean and disinfect the cryo chamber - see Recommended Disinfection Procedure. ...
  • Page 119 Index Bringing the instrument back into service: After moving the CryoStar NX70 / NX50, wait at least 8 hours before switching on to allow the refrigerant to settle - failure to do so may cause damage to the unit. ...

  • Page 120: Appendices

    Appendices...

  • Page 121: Appendix A - Spares And Accessories

    Index Appendix A - Spares and Accessories Description Part Number Knife and Blade Holder Standard Knife Holder Disposable Blade Holder for high profile blades Disposable Blade Holder for low profile blades Heat Extractor Specimen Chucks Ø 20 mm 715530 Ø 30 mm 715600 Ø...
  • Page 122 Description Part Number Foot pedal (optional) Conventional Knives Profile C; 16 cm 152020 Profile D; 16 cm 152070 Knife Case for 2 Knives, 16 cm 152230 Disposable Blades High Profile Blades, 50 pcs. in dispenser 152450 Low Profile Blades SEC 35, 50 pcs. in 152200 dispenser Low Profile Blades SEC 35e, 50 pcs.

  • Page 123: Appendix B - Recommended Disinfection Procedure

    Disinfection is the destruction of pathogens by physical or chemical means. It is not as fully effective as sterilization. Disinfection destroys most recognized pathogens, but not necessarily all microbial forms, such as bacterial spores. Disinfection Procedure for the Cryostar Nx70 cryostat  Wear appropriate protective clothing: double gloves, gown, mask and protective eye wear.

  • Page 124: Index

    Index A  F  Anti Roll Plate ........16, 20, 27 Favorites ........... 8, 45, 64 B  H  Blade Clamping Lever ....... 17, 21, 23, 24 Heated Window .......... 35, 77 Blade Holder17, 20, 21, 23, 24, 25, 41, 45, 91, 105, Height Adjustment ........
  • Page 125 Index W  Waste Tray ............16...

This manual is also suitable for:

Cryostar nx50

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