Chapter 1 Nanodrop Lite Plus User Guide - Thermo Scientific NanoDrop Lite Plus User Manual

Micro-uv spectrophotometer
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NanoDrop Lite Plus User Guide
Thermo Scientific
The Thermo Scientific™ NanoDrop™ Lite Plus is a small, stand-alone UV
spectrophotometer developed specifically for microvolume measurements of nucleic
acids and purified proteins. The NanoDrop Lite Plus comes with preloaded software
and is designed to be used without a computer. The NanoDrop Lite Plus can also be
connected to an accessory printer for printing measurement information on labels.
The sample retention system of the NanoDrop Lite Plus is identical to that used in all
NanoDrop instruments: surface tension is used to hold 1-2 μL of sample between
two stainless steel pedestals that contain optical fibers. The patented sample
retention system enables the measurement of highly concentrated samples without
the need for dilutions.
The NanoDrop Lite Plus measures the sample absorption at 230, 260, and 280 nm
and corrects for scattering and background effects in the sample with light from a
reference wavelength at 340 nm.
NanoDrop Lite Plus User Guide
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