Sigma 1-14K Operating Manual
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Sigma 1-14K Operating Manual

Refrigerated centrifuge
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Operating Manual
1-14K
Refrigerated Centrifuge
from serial no. 135550
Please retain for later use!
Version 05/2010, Rev. 1.8 of 01/09/2011- sb
Translation of the original operating manual

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Summary of Contents for Sigma 1-14K

  • Page 1 Operating Manual 1-14K Refrigerated Centrifuge from serial no. 135550 Please retain for later use! Version 05/2010, Rev. 1.8 of 01/09/2011- sb Translation of the original operating manual...
  • Page 3 1-14K In case of inquiries please state the following number: Serial number: © Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Phone +49 (0) 55 22 / 50 07-0 +49 (0) 55 22 / 50 07-12 E-mail: info@sigma-zentrifugen.de...

  • Page 5: Table Of Contents

    1-14K Table of Contents Table of Contents General Information Importance of the Operating Manual ................9 Intended Use ......................9 Warranty and Liability ....................9 Copyright........................10 Standards and Regulations ..................10 Scope of Supply ....................... 10 Design of the Centrifuge Overview ........................
  • Page 6 1-14K Table of Contents Set-up and Connection Unpacking the Centrifuge ..................21 Transport Safety Device ................... 21 Installation Site ......................21 Power Supply ......................22 5.4.1 Connection....................22 5.4.2 Fuses on site ....................22 Using the Centrifuge Initial Start-Up......................23 Switching the Centrifuge ON..................
  • Page 7 1-14K Table of Contents Maintenance and Service Maintenance......................36 8.1.1 Centrifuge ....................36 8.1.2 Accessories ....................37 8.1.2.1 Plastic Accessories ..............37 8.1.2.2 Aluminium Accessories ............... 37 8.1.3 Rotors......................37 8.1.4 Microhematocrite Rotor................38 Sterilization and Disinfection of the Rotor Chamber and Accessories....... 38 8.2.1.

  • Page 9: General Information

    They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. SIGMA Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use. The intended use also includes •...

  • Page 10: Copyright

    1-14K General Information Copyright The copyright concerning the operating manual remains Sigma Laborzentrifugen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that may not be • duplicated, • distributet, or •...

  • Page 11: Design Of The Centrifuge Overview

    1-14K Design of the Centrifuge Design of the Centrifuge Overview Display Set key Program key Arrow keys Precool key Start / stop key Lid key Fig. 2.1: Total view of the centrifuge Mains power input Mains switch Name Plate (see 2.2) Fig.

  • Page 12: Safety

    1-14K Safety Safety Marking of the Unit International symbols used for SIGMA centrifuges: Gefährliche elektrische Spannung Dangerous voltage Courant haute tension Achtung, Betriebsanleitung lesen Attention, consult instruction manual Attention, consulter mode d'emploi Ein (Netzverbindung) On (Power) Marche (mise sous tension)

  • Page 13: Explanation Of The Symbols And Notes

    1-14K Safety Explanation of the Symbols and Notes This operating manual uses the following names and symbols to indicate hazards: This symbol stands for a direct hazard to the life and health of persons. Non-observance of these symbols causes serious health problems up to life-endangering injuries.

  • Page 14: Responsibility Of The Operator

    1-14K Safety Responsibility of the Operator The operator is responsible to authorise only qualified personnel to work on the machine (see chapter 3.4 "Operating Personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.

  • Page 15: Safety Instructions

    • Stop the centrifuge immediately in the event of a malfunction (see chapter 7 "Malfunctions and Error Correction") or contact the service of SIGMA Laborzentrifugen GmbH (see 7.3 "Service Contact"). • Ensure that all repairs are performed only by authorized and specialized personnel (see chapter 7.3 "Service Contact").

  • Page 16: Fire Prevention

    1-14K Safety • Prior to any start-up, check the centrifuge, rotor, and accessories for signs of damage that can be discerned from the outside. Special attention must be paid to all of the rubber parts (e.g. motor cover, lid seal, adapter) in terms of visible structural changes.

  • Page 17: Safety Instructions For Centrifugation

    1-14K Safety 3.6.5 Safety Instructions for Centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 "Set-up and Connection“). • Maintain a safety distance of at least 30 cm (12 inches) around the centrifuge.

  • Page 18: Safety Devices

    1-14K Safety or accessory, these data shall apply accordingly. For example, a bucket with an engraving "max.cycles = 10.000" has a service life of 10.000 cycles; and a rotor with the engraving "Exp.Date 02/15" must be scrapped in February 2015 at the latest (see fig. 3.1).

  • Page 19: Measures In The Event Of Hazards And Accidents

    1-14K Safety Measures in the Event of Hazards and Accidents • If an emergency arises, switch off the centrifuge immediately! • If in doubt, call the emergency doctor! Remaining Hazards The unit was built state-of-the-art and according to the accepted safety rules.

  • Page 20: Storage And Transport Dimensions And Weight

    1-14K Storage and Transport Storage and Transport Dimensions and Weight Height: 236 mm Height with opened lid: 404 mm Width: 273 mm Depth: 515 mm Weight: 19 kg Fig. 4.1: Dimensions and weight Storage Conditions The centrifuge can be stored for up to a year without any problems.

  • Page 21: Set-Up And Connection Unpacking The Centrifuge

    1-14K Set-up and Connection Set-up and Connection Unpacking the Centrifuge The centrifuge is packaged in a cardboard box. • Open the box. • Take out the accessories. • Remove the upper foam cushion. • Lift the centrifuge with both foam cushions out of the cardboard box.

  • Page 22: Power Supply

    1-14K Set-up and Connection Power Supply 5.4.1 Connection The operating voltage on the name plate must correspond to the local supply voltage! • This laboratory centrifuge is a unit of safety class I SIGMA and has a three-wire power cord with a IEC C13 connector.

  • Page 23: Using The Centrifuge Initial Start-Up

    1-14K Using the Centrifuge Using the Centrifuge Initial Start-Up Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 "Set-up and Connection"). Switching the Centrifuge ON • Press the mains power switch (see Fig. 2.2, page 11).

  • Page 24: Installation Of The Microhematocrite Rotor

    1-14K Using the Centrifuge • Open the centrifuge lid by pressing the lid key. • Unscrew the rotor fastening nut from the motor shaft (counter- clockwise). • Lower the rotor with its central bore straight down onto the motor shaft.

  • Page 25: Installation Of Accessories

    1-14K Using the Centrifuge 6.2.2.3 Installation of Accessories • Only use inserts that are suitable for the rotor (see 11.1 "Suitable Accessories"). • Always load the opposite inserts/buckets of the rotors with the same accessories and fill to avoid imbalance.

  • Page 26: Operation

    1-14K Using the Centrifuge Operation 6.3.1 Operating Panel 1 Centrifuge display 2 Set key 3 Program key 4 Arrow key 5 Precool key 6 Start/Stop key 7 Lid key Fig. 6.3: Operating Panel The centrifuge is started directly via the operating panel. When the centrifuge is switched on, all segments will be illuminated for a short time.

  • Page 27: Starting A Centrifugation Run

    1-14K Using the Centrifuge 6.3.3 Starting a Centrifugation Run The centrifuge is ready for operation when the mains power switch is on and the lid is closed. • Press the start key in order to start a centrifugation run. 6.3.4 Interrupting a Centrifugation Run •...

  • Page 28: Runtime

    1-14K Using the Centrifuge 6.3.7 Runtime The preselected total runtime is displayed in the lower line of the display. During centrifugation, the remaining runtime is displayed. The runtime of the centrifuge can be set at one-second-intervals up to 99 minutes and 59 seconds.

  • Page 29: Temperature

    1-14K Using the Centrifuge 6.3.7.4 Temperature The temperature in the rotor chamber is displayed in the lower third of the centrifuge display. The display alternates between the preselected temperature and the actual temperature. The actual temperature is marked by the word “actual“in front of the temperature value.

  • Page 30: Softstart And Softstop Function

    1-14K Using the Centrifuge 6.3.7.6 Softstart and Softstop Function The softstart function is used to extend the acceleration time, whereas the softstop function is used to extend the deceleration time. The current combination is shown on the display. • To activate the softstart and softstop functions, press the “set”...

  • Page 31: Switching The Centrifuge Off

    1-14K Using the Centrifuge 6.3.9 Programs The program is used to save or load certain recurrent settings of the centrifuge. 10 different programs can be saved and called up. The precooling program "precool" does not occupy any storage location and cannot be deleted. It is used to cool the centrifuge without vessels (see 6.3.7.5).

  • Page 32: Malfunctions And Error Correction 7.1 General Malfunctions

    1-14K Malfunctions and Error Correction Malfunctions and Error Correction General Malfunctions Malfunctions are indicated by error messages with a number in the speed display. In the event of a fatal error (e.g. a defective lid lock), a certain safety time will be counted down on the display. During this time, “ERR” and “SAFE”...

  • Page 33: Emergency Lid Release

    1-14K Malfunction and Error Correction 7.1.1 Emergency Lid Release In the event of a power failure, it is possible to manually open the centrifuge lid with a socket wrench (part no. 930 014, included in the scope of supply). The opening is located in the bottom plate in the front quarter on the left-hand side.

  • Page 34: Error Codes

    1-14K Malfunctions and Error Correction Error Codes Error no. Kind of error Measure Note • allow to slow down System error All these errors stop the • power off/on centrifuge or cause it to decelerate brakeless • allow to slow down...

  • Page 35: Service Contact

    → [Service Area] www.sigma-zentrifugen.de • or contact SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-84 25 Fax +49 (0) 55 22 / 50 07-94 25 E-mail: service@sigma-zentrifugen.de...

  • Page 36: Maintenance And Service

    1-14K Maintenance and Service Maintenance and Service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure. If corrosion or other damage occurs due to improper care, the manufacturer cannot be held liable or subject to any warranty claims.

  • Page 37: Accessories

    1-14K Maintenance and Service 8.1.2 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at the same time. • Immediately rinse off the rotor, buckets, or accessories under running water if they have come into contact with any liquids that may cause corrosion.

  • Page 38: Microhematocrite Rotor

    1-14K Maintenance and Service Check the material regularly (at least once a month) for - cracks - visible damage of the surface - pressure marks - signs of corrosion - other changes. • Check the bores of the rotors. •...

  • Page 39: 8.2.1. Autoclaving

    1-14K Maintenance and Service 8.2.1. Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in color or structure or in the occurrence of leaks etc.

  • Page 40: 8.3 Service

    → [Service Area] www.sigma-zentrifugen.de • or contact SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-84 25 Fax +49 (0) 55 22 / 50 07-94 25 E-mail: service@sigma-zentrifugen.de...

  • Page 41: Return Of Defective Parts

    1-14K Maintenance and Service Return of defective parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer. In order to ensure the quick and economical processing of returns of centrifuges, spare parts, or accessories, we require complete and extensive information concerning the process.

  • Page 42: Disposal

    This symbol means that it is not permissible to dispose of the unit among household trash. • You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH. • Ensure that the unit is decontaminated. Fill in a declaration of decontamination.

  • Page 43: Technical Data

    1-14K Technical Data Technical Data Manufacturer: S I G M A Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: 1-14K Electr. Connection: see name plate Protection class: IP Code: Connected load (kVA): 0.32 Power consumption (kW): 0.23 Max.

  • Page 44: Appendix

    1-14K Appendix Appendix 11.1 Suitable Accessories Part no. Description Max. speed Max. (rpm) gravitational field (x g) 12082 Angle rotor 12 x 1.5/2.0 ml, for reaction vials, e.g. no. 15 000 15 596 15008, 15040, incl. polysulfone lid no. 17882, radius max.
  • Page 45 1-14K Appendix Adapters and plastic tubes Part no. Description 13021 Adapter for PCR-tube 0.2 ml, e.g no. 15042, 1 set = 2 pcs. 13000 Adapter for reaction vials 0.25/0.4 ml no. 15014, 1 set = 2 pcs. 13002 Adapter for reaction vials 0.5/0.75 ml, Ø 7.9/10 x 28/31 mm, e.g. no. 15005, 1 set = 2 pcs.

  • Page 46: 11.2 Grafical Representation Of The Rotors

    1-14K Appendix 11.2 Grafical Representation of the Rotors The graphical representation of the rotors shows the maximum and minimum radii of the accessories used. If necessary, the values must be manually calculated (see 11.3.1 "RCF“). Fig 11.1: Minimum and maximum radius of an angle rotor Abb 11.2:...

  • Page 47: 11.3 Formulae - Mathematical Relations

    1-14K Appendix 11.3 Formulae - Mathematical Relations 11.3.1 Relative Centrifugal Force (RCF) The parameters speed, RCF, and the group rotor and radius cannot be specified independently. They are interrelated via the following formula: RCF = 11.18 x 10-6 x r x n2 If two values are given, the third value is determined by the equation.
  • Page 48 1-14K Appendix Fig. 11.3: Speed-Grravitational-Field Diagram Version 05/2010, Rev. 1.8 of 01/09/2011- sb Translation of the original operating manual...

  • Page 49: Table Of Rotors And Accessories With A Different Service Life

    Rotor / Cycles Service life Autoclaving Suitable for centrifuge Remarks bucket ("Exp.Date") 11026 7 years 1-14, 1-14K 12082 7 years 1-14, 1-14K 12083 7 years 1-14, 1-14K 12084 7 years 1-14, 1-14K 12085...

  • Page 50: Resistance Data

    1-14K Appendix Resistance Data Resistance Data Resistant at +20 ° C no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated Acetone Acrylonitrile Allyl alcohol Aluminum chloride AlCl saturated Aluminum sulfate Ammonium chloride...
  • Page 51 1-14K Appendix Resistant at +20 ° C no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Dioxane Dipropylene glycol (mono)methyl ether Ethyl acetate Ethylene chloride Ferrous chloride FeCl saturated Formaldehyde solution Formic acid Furfural...
  • Page 52 1-14K Appendix Resistant at +20 ° C no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Potassium hydrogen carbonate CHKO saturated Potassium hydroxide Potassium hydroxide Potassium nitrate Potassium permanganate KMnO Pyridine Resorcinol Silver nitrate...

  • Page 53: Ec-Declaration Of Conformity

    EC-Declaration of Conformity...
  • Page 55 1-14K Index Index Acceleration time - see also the softstart and softstop function..............30 Accessories for microhematocrite capillary tubes..............45 Accident prevention ......................14 Acid ........................17, 36, 50, 52 Adapters ..........................38 Adapters and plastic tubes....................45 Alkaline solutions ......................17, 37 Allowable ambient temperature....................
  • Page 56 1-14K Index Dimensions and weight ......................20 Direct hazard to the life and health..................13 Disinfectants ........................38 Disposal of the centrifuge..................... 42 Disposal of the packaging ....................42 Documentation........................10 EC-Declaration of Conformity ....................53 EEPROM error........................34 Electrical connection ......................43 Electrical safety........................
  • Page 57 1-14K Index Maintenance ........................36 Malfunction ........................18, 32 Malfunctions ........................15 Malfunctions and error correction..................32 Manufacturer ........................43 Marking of the unit ....................... 12 Max. capacity........................43 Max. current consumption....................43 Max. gravitational field ......................43 Max. kinetic energy......................11, 43 Max.
  • Page 58 1-14K Index Remaining hazards ......................19 Resistance data ....................... 17, 37, 50 Resistance of plastics ......................17 Responsibility of the operator....................14 Return of centrifuges, spare parts, or accessories ............... 41 Return of defective parts ...................... 41 Rotor chamber ........................36 Rotor fastening nut......................
  • Page 59 1-14K Index Temperature error........................ 34 Toxic substances ..................... 16, 36, 37 Transport safety device......................21 Tubes ..........................25 Type ............................ 43 Type name........................... 11 Type of the centrifuge ....................35, 40 Unpacking the centrifuge ..................... 21 Using the centrifuge......................23 UV radiation........................

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