Operation - Agilent Technologies SD-1 User Manual

4
Using the SD-1 Solvent Delivery Module

Operation

Operation

Flushing the HPLC System
Before pumping solvent through an HPLC system, it is important to flush all
tubing to prevent impurities or particulates from the new tubing (such as dust,
cutting oils, metal swarf, etc.) from clogging the column frit or contaminating
the column.
Chemical Hazard
WARNING
➔
1 Disconnect the tubing fitting at the column inlet and place the fitting into a
2 To set the FLOW to 15 mL/min on each Agilent SD-1 pump, press 15 and
3 Press RUN to start each pump.
4 Pump solvent through the tubing for several minutes.
Checking for Leaks Against System Backpressure
So far the pump has been operated without solvent flowing through the
column. Before using the Agilent SD-1 pump in actual chromatography you
should check all connections for leaks by pumping against column
backpressure.
1 Immerse the solvent inlet filter into a suitable HPLC-grade solvent for the
42
Danger of burns. To avoid injury to personnel or damage to equipment, always
follow appropriate safety guidelines when using chemicals and always wear
appropriate safety equipment and clothing.
waste container. It is vital that the tubing is thoroughly flushed before flow
is introduced to the column.
confirm with ENTER.
The tubing is thoroughly flushed before flow is introduced to the column.
column in use. Ensure that the solvent is miscible with the last-used solvent
(water if you have followed the procedures so far in this manual). If the
SD-1 Solvent Delivery - User Manual