Agilent Technologies SD-1 User Manual page 57

5
Optimizing Performance
System Requirements for High Performance Prep Chromatography
Figure 33 Pressure capacity and flow rate diagram
A pressure capacity of 6000 psi (410 bar) is maintained up to 150 mL/min
where it decreases to 4000 psi (274 bar) by 200 mL/min - more than ample for
HPLC.
Not only are high flow and pressure required for prep HPLC, linear flow and
adequate mixing are also required for reproducible chromatography of
proteins and peptides.
Agilent SD-1 pump with 200 mL/min heads works well at both high and low
flow rates. The following figures reveals that excellent flow rate linearity for
low flow rates is achieved, and just as important is the excellent flow
reproducibility for flow rates from 80 – 150 mL/min.
SD-1 Solvent Delivery - User Manual 57