Hints For Successful Use Of The Pump - Agilent Technologies SD-1 User Manual

4
Using the SD-1 Solvent Delivery Module

Hints for Successful Use of the Pump

Hints for Successful Use of the Pump

• Use only HPLC-grade solvents. Impurities present in other solvents may
• The Agilent SD-1 piston washing feature is useful when using high-salt
• As well as washing the pistons, after using high-salt buffers always pump at
• Never let the Agilent SD-1 stand overnight with high-salt buffer solution in
• Do not change directly between immiscible solvents; any resulting
• Always use the Agilent SD-1 pressure-limiting feature to protect the HPLC
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clog the frits in the check valves and column. Always use a solvent inlet
filter, to prevent particulate material from entering the Agilent SD-1 and
clogging the frits.
buffers. Piston washing prevents salt crystallization behind the seal. If salt
residues were to accumulate, seal life would be greatly reduced (see
"Cleaning Check Valves"
least 50 mL of HPLC-grade water through the pumping system to flush
away salt deposits from the pump heads and outlet lines.
the pump heads. If this should happen, it is important to wash the pistons
and flush the pump heads with water at a low flow rate before starting
work.
precipitation would be extremely difficult to remove from the pump heads,
check valves, and tubing (see
solvent you wish to use is immiscible with the solvent in the system change
to it via another solvent (or a series) that is miscible with both.
column from overpressure which could damage the column and impair
performance (see "Setting the Maximum Pressure"
dynamic pressure is always shown in the top line of the display. It is good
practice to monitor this value and be prepared to stop the pump should
pressure rise rapidly.
on page 95).
"Solvent Miscibility"
SD-1 Solvent Delivery - User Manual
on page 120). If the
on page 44). The