Agilent Technologies SD-1 User Manual page 68

6
Troubleshooting and Diagnostics
Troubleshooting Guide
Drifting baseline
Table 17
Probable cause
Contaminated flow cell
Localized temperature effects
Contamination in column
Leak in system
Bubble trapped in flow cell
Column not equilibrated
Mobile phase contamination
Weak detector lamp
Flat-top peaks
Table 18
Probable cause
Saturated electronics
Recorder adjusted incorrectly
Bad grounding
68
Drifting baseline
Flat-top peaks
Suggested actions
Attach a syringe to the flow cell inlet and try to
clear blockage by drawing on the syringe. Or
attach to outlet and back-flush to clear blockage
by gentle pressure on the syringe. Do not apply
pressure to the flow cell inlet.
• Wrap tubing, column
• Remove or cover heat or cooling source
• Wash or replace column
• Change mobile phase
Locate leak and repair.
• Flush flow cell
• Degas solvent
• Add back-pressure device to flow cell
Flush system until column is equilibrated.
Use fresh HPLC-grade solvents.
Replace detector lamp.
Suggested actions
Reduce sample volume.
Adjust diameter offset or range.
Check all grounding connections on the module
and ensure grounded AC power is supplied to
all devices in HPLC system.
SD-1 Solvent Delivery - User Manual