Nikon SMZ800N Instruction Manual page 63

 Filter cube
 P-GFP-B Filter Cube and P-GFP-L Filter Cube: Used for fluorescence observation at a given wavelength.
 P-EFLC Filter Cube: Used for fluorescence observation at an arbitrary wavelength when combined with arbitrary filters,
for example.
The sizes of the attachable filters and mirror are listed below:
Excitation filter (EX):
Dichroic mirror (DM):
Barrier filter (BA):
For details, refer to Chapter 5, "3.2 Assembly of Other Devices (Optional) - [3] Attach a P-EFL Epi Fluorescence
Attachment."
 P2-EFLBF Filter Cube (Bright Field): Used for episcopic bright-field observation.
 Empty cube (Three cubes are supplied with an epi-fluorescence attachment): Used when performing diascopic
illumination observation using the epi-fluorescence attachment.
Light shielding plate
The device is equipped with a light shielding plate for preventing the reflection of ultraviolet light. Use the light shielding
plate when performing fluorescence microscopy. When in use, the light shielding plate can be pushed aside to positions
up to 45° horizontally to the rear. When not in use, the plate can be pushed aside to positions up to 170°.
Episcopic bright-field observation
This is useful for viewing the entire image of an opaque specimen during fluorescence observation.
Even though the P-EFLBF Filter Cube has a built-in UV-cut filter, adjust the light intensity to the appropriate level with the
light source illuminator so that illumination is not too bright. In addition, two types of 1/4 λ plates are provided in order to
prevent flares at the imaging system caused by the light reflected inside the optical system: the 1/4 λ plate for 1x or lower
power objectives, and the 1/4 λ plate for 1x objectives. Attach a corresponding 1/4 λ plate to the tip of each objective.
Note the following points when using a P-EFLBF Filter Cube:
 Brightness differs between the right and left optical path when reflected light from mirror plane type specimens is
observed.
 Double image may occur due to the built-in half mirror.
 The periphery of the image may not be clearly visible in the low zoom magnification range.
 The periphery of the image may become dark when an intermediate tube such as a beam splitter is used together.
OCC light shielding plate
When using an epi-fluorescence attachment in combination with a P-DSL32 LED Diascopic Illumination Stand, Nikon
recommends closing the OCC light shielding plate on the dia-illumination completely. This can decrease background noise
caused by intrinsic fluorescence in the diascopic illuminator.
* The P-EFL Epi Fluorescence Attachment cannot be used together with the microscope system that includes a
C−PSN/C-PSCN Plain Stand, a C-DS Dia Illumination Stand or a C-LEDS Hybrid LED Stand.
Chapter 4
Functions and Operations of the Devices
External diameter 18 mm, Thickness 8 mm or less
Dimensions 18 mm x 26 mm, Thickness 1 mm or less
External diameter 18 mm, Thickness 3.8 mm or less
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