Objectives; Fig. 1-3 Objective - Zeiss Axioskop 2 FS plus Manual

Carl Zeiss
Fig. 1-3 Objective
Other labels:
Oil : oil immersion objective
Ph1 : phase contrast objective with a green color ring and phase stop Ph1
W : objectives for water immersion
Color ring code for objective magnification
Color ring on black
objective
Magnification 1.25x
factor
The objective magnification multiplied with the eyepiece magnification (minimum 10x) results in the
visual overall magnification: e.g. 10 x 10 = 100x.
The overall magnification should not exceed or fall below the useful magnification range. The useful
magnification range was defined by Ernst ABBE as 500 to 1000 times the numerical aperture of the
objective used. No further details are resolved above that limit. For an objective with a numerical
aperture of 0.25, the range of useful magnification is between 125 and 250x.
1-12
INSTRUMENT DESCRIPTION

Objectives

brown red orange
2.5x 4x; 5x 6.3x
1.5 Objectives
The objectives are the optical centerpiece of the
microscope. The following is an example of how
objectives can be labelled:
Achroplan 10x/0,3W Ph1 ∞/−
where
10x : objective magnification, with a defined
color ring on the objective being allocated
to each magnification step (Zeiss color
code)
0.3 : numerical aperture
∞
: infinite image distance; these objectives
can only be used with ICS microscopes
from Carl Zeiss
−
: can be used with cover slip thickness D = 0
or 0.17 mm
or
0.17 : can only be used with cover slip thickness
D = 0.17 mm
yellow green
10x 16x; 20x;
25x; 32x
Axioskop 2 FS plus
Axioskop 2 FS
light blue dark blue white
40x; 50x 63x 100x;
150x
B 40-076 e
MOT
02/01