Zeiss Axioskop 2 FS plus Manual page 91

Axioskop 2 FS plus
Axioskop 2 FS
MOT
• Swing in 10x objective (yellow ring, see p. 1-12)
in the objective slider (3-16/5) and focus on the
specimen via focusing drive (3-16/1).
• Close luminous-field diaphragm (3-16/3) until it
becomes visible (even if not in focus) in the field
of view (3-16/A).
• Use drive for height adjustment (3-17/2) to
lower condenser until the edge of the
luminous-field diaphragm appears in focus
(3-16/B).
• Use both centering screws (3-17/3) of the
universal condenser to center the luminous-field
diaphragm (3-16/C)
diaphragm until its edge just disappears from
the field of view (3-16/D).
• For aperture diaphragm setting (contrast),
remove one eyepiece from the tube and look
into the tube with your naked eye. Use sliding
knob (3-16/4) to set the aperture diaphragm to
approx. 2/3 ... 4/5 of the diameter of the
objective exit pupils
applications, this aperture diaphragm setting
provides optimum contrast at almost ideal
resolution, and
compromise for the human eye.
• Insert eyepiece in the tube again.
Since field size and objective aperture change after every objective change, the setting of the
luminous-field diaphragm and the aperture diaphragm must be repeated to obtain optimum
results.
For < 10x objectives, the front lens of the condenser must be folded out and the aperture
diaphragm fully opened. In the case of such large fields, the luminous-field diaphragm can also
be used for better contrasting by reducing its opening until it becomes visible in the field of
view.
B 40-076 e 02/01
Illumination and contrasting techniques
and then open
(3-16/E). In
is therefore the
OPERATION
the
most
best
Fig. 3-16 Microscope settings on the
Axioskop 2 FS plus in transmitted-
light brightfield
Carl Zeiss
3-21