Preparing The Device For Operation - Thermo Scientific thermoscientific Vanquish Pumps C Operating Manual

6.6

Preparing the Device for Operation

Before Operating the Device for the First Time
Before Starting Sample Analysis
Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
This section gives information on any additional steps that are required
to prepare the device for operation and sample analysis.
Prepare the device for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for
the first time:
• When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the Vanquish
System Operating Manual.
• Some components of the device are filled with isopropanol when
the device is shipped from the manufacturing site. When operating
the device for the first time, use solvents that are miscible with
isopropanol. If they are not, use an appropriate intermediate
solvent.
• To remove the isopropanol from the device components:
Verify that the flow cell and the column are not connected in the
¨
flow path.
Purge the pump and then have the pump deliver flow for a short
¨
time.
• Verify that any air bubbles are completely flushed out of the system
flow path.
• Quaternary pump and dual pump: Synchronize the injection time of
the Vanquish autosampler with the strokes of the pump. See
Synchronizing the Injection Time with the Pump Strokes
(} page 224).
Before starting an analysis:
• Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
• Verify that the doors of all modules in the Vanquish system are
closed.
• Make sure that the chromatography system is properly equilibrated
(see further down).
6 • Operation
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